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Susceptibility of Oocytes from Gilts and Sows to Beauvericin and Deoxynivalenol and Its Relationship with Oxidative Stress
Beauvericin (BEA) and deoxynivalenol are toxins produced by Fusarium species that can contaminate food and feed. The aim of this study was to assess the effects of these mycotoxins on the maturation of oocytes from gilts and sows. Furthermore, the antioxidant profiles in the oocytes’ environment wer...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067504/ https://www.ncbi.nlm.nih.gov/pubmed/33917490 http://dx.doi.org/10.3390/toxins13040260 |
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author | Schoevers, Eric J. Santos, Regiane R. Roelen, Bernard A. J. |
author_facet | Schoevers, Eric J. Santos, Regiane R. Roelen, Bernard A. J. |
author_sort | Schoevers, Eric J. |
collection | PubMed |
description | Beauvericin (BEA) and deoxynivalenol are toxins produced by Fusarium species that can contaminate food and feed. The aim of this study was to assess the effects of these mycotoxins on the maturation of oocytes from gilts and sows. Furthermore, the antioxidant profiles in the oocytes’ environment were assessed. Cumulus-oocyte-complexes (COCs) from gilts and sows were exposed to beauvericin (BEA) or deoxynivalenol (DON) and matured in vitro. As an extra control, these COCs were also exposed to reactive oxygen species (ROS). The maturation was mostly impaired when oocytes from gilts were exposed to 0.02 μmol/L DON. Oocytes from sows were able to mature even in the presence of 5 μmol/L BEA. However, the maturation rate of gilt oocytes was already impaired by 0.5 μmol/L BEA. It was observed that superoxide dismutase (SOD) and glutathione (GSH) levels in the follicular fluid (FF) of gilt oocytes was higher than that from sows. However, the expression of SOD1 and glutathione synthetase (GSS) was higher in the oocytes from sows than in those from gilts. Although DON and BEA impair cell development by diverse mechanisms, this redox imbalance may partially explain the vulnerability of gilt oocytes to these mycotoxins. |
format | Online Article Text |
id | pubmed-8067504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80675042021-04-25 Susceptibility of Oocytes from Gilts and Sows to Beauvericin and Deoxynivalenol and Its Relationship with Oxidative Stress Schoevers, Eric J. Santos, Regiane R. Roelen, Bernard A. J. Toxins (Basel) Article Beauvericin (BEA) and deoxynivalenol are toxins produced by Fusarium species that can contaminate food and feed. The aim of this study was to assess the effects of these mycotoxins on the maturation of oocytes from gilts and sows. Furthermore, the antioxidant profiles in the oocytes’ environment were assessed. Cumulus-oocyte-complexes (COCs) from gilts and sows were exposed to beauvericin (BEA) or deoxynivalenol (DON) and matured in vitro. As an extra control, these COCs were also exposed to reactive oxygen species (ROS). The maturation was mostly impaired when oocytes from gilts were exposed to 0.02 μmol/L DON. Oocytes from sows were able to mature even in the presence of 5 μmol/L BEA. However, the maturation rate of gilt oocytes was already impaired by 0.5 μmol/L BEA. It was observed that superoxide dismutase (SOD) and glutathione (GSH) levels in the follicular fluid (FF) of gilt oocytes was higher than that from sows. However, the expression of SOD1 and glutathione synthetase (GSS) was higher in the oocytes from sows than in those from gilts. Although DON and BEA impair cell development by diverse mechanisms, this redox imbalance may partially explain the vulnerability of gilt oocytes to these mycotoxins. MDPI 2021-04-06 /pmc/articles/PMC8067504/ /pubmed/33917490 http://dx.doi.org/10.3390/toxins13040260 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Schoevers, Eric J. Santos, Regiane R. Roelen, Bernard A. J. Susceptibility of Oocytes from Gilts and Sows to Beauvericin and Deoxynivalenol and Its Relationship with Oxidative Stress |
title | Susceptibility of Oocytes from Gilts and Sows to Beauvericin and Deoxynivalenol and Its Relationship with Oxidative Stress |
title_full | Susceptibility of Oocytes from Gilts and Sows to Beauvericin and Deoxynivalenol and Its Relationship with Oxidative Stress |
title_fullStr | Susceptibility of Oocytes from Gilts and Sows to Beauvericin and Deoxynivalenol and Its Relationship with Oxidative Stress |
title_full_unstemmed | Susceptibility of Oocytes from Gilts and Sows to Beauvericin and Deoxynivalenol and Its Relationship with Oxidative Stress |
title_short | Susceptibility of Oocytes from Gilts and Sows to Beauvericin and Deoxynivalenol and Its Relationship with Oxidative Stress |
title_sort | susceptibility of oocytes from gilts and sows to beauvericin and deoxynivalenol and its relationship with oxidative stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067504/ https://www.ncbi.nlm.nih.gov/pubmed/33917490 http://dx.doi.org/10.3390/toxins13040260 |
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