Cargando…

Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses

Administration of albendazole alone was not very suitable for the treatment of cerebral angiostrongyliasis. This study was designed to evaluate the effects of the co-therapy of this drug and dexamethasone in Th-1 and Th-2 dominant mice infected with Angiostrongylus cantonensis. Each of BALB/c and C5...

Descripción completa

Detalles Bibliográficos
Autores principales: Jhan, Kai-Yuan, Cheng, Chien-Ju, Jung, Shih-Ming, Lai, Yi-Jen, Chen, Kuang-Yao, Wang, Lian-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067505/
https://www.ncbi.nlm.nih.gov/pubmed/33917604
http://dx.doi.org/10.3390/biom11040536
_version_ 1783682819349807104
author Jhan, Kai-Yuan
Cheng, Chien-Ju
Jung, Shih-Ming
Lai, Yi-Jen
Chen, Kuang-Yao
Wang, Lian-Chen
author_facet Jhan, Kai-Yuan
Cheng, Chien-Ju
Jung, Shih-Ming
Lai, Yi-Jen
Chen, Kuang-Yao
Wang, Lian-Chen
author_sort Jhan, Kai-Yuan
collection PubMed
description Administration of albendazole alone was not very suitable for the treatment of cerebral angiostrongyliasis. This study was designed to evaluate the effects of the co-therapy of this drug and dexamethasone in Th-1 and Th-2 dominant mice infected with Angiostrongylus cantonensis. Each of BALB/c and C57BL/6 mice infected with 50 A. cantonensis third-stage larvae were administered albendazole (10 mg/kg/day) alone, dexamethasone (0.5 mg/kg/day) alone, or co-therapy of the two drugs from day 7 or 14 post-infection for 7 or 14 days. After sacrifice, coronal slices were prepared from five brain regions and stained with hematoxylin and eosin. Eight pathological changes were employed to determine the therapeutic effectiveness using a scoring system. RNA-seq analysis was performed to confirm the histopathological findings. The infected BALB/c and C57BL/6 mice had similar patterns in the pathological changes. Meningitis, hemorrhage, size of worms, and encephalitis in the cerebral parenchyma were slighter in the mice treated with co-therapy than the remaining groups. Mice treated from day 14 had more severe changes than those from day 7. The histopathological findings were found to be consistent to immune responses determined by RNA-seq analysis. Co-therapy was determined to reduce pathological changes after administration to mice infected with A. cantonensis.
format Online
Article
Text
id pubmed-8067505
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80675052021-04-25 Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses Jhan, Kai-Yuan Cheng, Chien-Ju Jung, Shih-Ming Lai, Yi-Jen Chen, Kuang-Yao Wang, Lian-Chen Biomolecules Article Administration of albendazole alone was not very suitable for the treatment of cerebral angiostrongyliasis. This study was designed to evaluate the effects of the co-therapy of this drug and dexamethasone in Th-1 and Th-2 dominant mice infected with Angiostrongylus cantonensis. Each of BALB/c and C57BL/6 mice infected with 50 A. cantonensis third-stage larvae were administered albendazole (10 mg/kg/day) alone, dexamethasone (0.5 mg/kg/day) alone, or co-therapy of the two drugs from day 7 or 14 post-infection for 7 or 14 days. After sacrifice, coronal slices were prepared from five brain regions and stained with hematoxylin and eosin. Eight pathological changes were employed to determine the therapeutic effectiveness using a scoring system. RNA-seq analysis was performed to confirm the histopathological findings. The infected BALB/c and C57BL/6 mice had similar patterns in the pathological changes. Meningitis, hemorrhage, size of worms, and encephalitis in the cerebral parenchyma were slighter in the mice treated with co-therapy than the remaining groups. Mice treated from day 14 had more severe changes than those from day 7. The histopathological findings were found to be consistent to immune responses determined by RNA-seq analysis. Co-therapy was determined to reduce pathological changes after administration to mice infected with A. cantonensis. MDPI 2021-04-06 /pmc/articles/PMC8067505/ /pubmed/33917604 http://dx.doi.org/10.3390/biom11040536 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jhan, Kai-Yuan
Cheng, Chien-Ju
Jung, Shih-Ming
Lai, Yi-Jen
Chen, Kuang-Yao
Wang, Lian-Chen
Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses
title Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses
title_full Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses
title_fullStr Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses
title_full_unstemmed Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses
title_short Co-Therapy of Albendazole and Dexamethasone Reduces Pathological Changes in the Cerebral Parenchyma of Th-1 and Th-2 Dominant Mice Heavily Infected with Angiostrongylus cantonensis: Histopathological and RNA-seq Analyses
title_sort co-therapy of albendazole and dexamethasone reduces pathological changes in the cerebral parenchyma of th-1 and th-2 dominant mice heavily infected with angiostrongylus cantonensis: histopathological and rna-seq analyses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067505/
https://www.ncbi.nlm.nih.gov/pubmed/33917604
http://dx.doi.org/10.3390/biom11040536
work_keys_str_mv AT jhankaiyuan cotherapyofalbendazoleanddexamethasonereducespathologicalchangesinthecerebralparenchymaofth1andth2dominantmiceheavilyinfectedwithangiostrongyluscantonensishistopathologicalandrnaseqanalyses
AT chengchienju cotherapyofalbendazoleanddexamethasonereducespathologicalchangesinthecerebralparenchymaofth1andth2dominantmiceheavilyinfectedwithangiostrongyluscantonensishistopathologicalandrnaseqanalyses
AT jungshihming cotherapyofalbendazoleanddexamethasonereducespathologicalchangesinthecerebralparenchymaofth1andth2dominantmiceheavilyinfectedwithangiostrongyluscantonensishistopathologicalandrnaseqanalyses
AT laiyijen cotherapyofalbendazoleanddexamethasonereducespathologicalchangesinthecerebralparenchymaofth1andth2dominantmiceheavilyinfectedwithangiostrongyluscantonensishistopathologicalandrnaseqanalyses
AT chenkuangyao cotherapyofalbendazoleanddexamethasonereducespathologicalchangesinthecerebralparenchymaofth1andth2dominantmiceheavilyinfectedwithangiostrongyluscantonensishistopathologicalandrnaseqanalyses
AT wanglianchen cotherapyofalbendazoleanddexamethasonereducespathologicalchangesinthecerebralparenchymaofth1andth2dominantmiceheavilyinfectedwithangiostrongyluscantonensishistopathologicalandrnaseqanalyses