Cargando…

Numerical Simulation and Experimental Verification of Droplet Generation in Microfluidic Digital PCR Chip

The generation of droplets is one of the most critical steps in the droplet digital polymerase chain reaction (ddPCR) procedure. In this study, the mechanism of droplet formation in microchannel structure and factors affecting droplet formation were studied. The physical field of laminar two-phase f...

Descripción completa

Detalles Bibliográficos
Autores principales:	Meng, Xiangkai, Yu, Yuanhua, Jin, Guangyong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067688/ https://www.ncbi.nlm.nih.gov/pubmed/33917077 http://dx.doi.org/10.3390/mi12040409

Ejemplares similares

Microdroplet PCR in Microfluidic Chip Based on Constant Pressure Regulation
por: Duanmu, Luyang, et al.
Publicado: (2023)

A Microfluidic Chip for Liquid Metal Droplet Generation and Sorting
por: Tian, Lu, et al.
Publicado: (2017)

Deep learning with microfluidics for on-chip droplet generation, control, and analysis
por: Sun, Hao, et al.
Publicado: (2023)

Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification: Design, Fabrication and Characterization
por: Coelho, Beatriz J., et al.
Publicado: (2023)

A Double-Deck Self-Digitization Microfluidic Chip for Digital PCR
por: Xu, Gangwei, et al.
Publicado: (2020)

Droplet Microfluidics for Chip-Based Diagnostics
por: Kaler, Karan V. I. S., et al.
Publicado: (2014)

Numerical Simulation and Experimental Validation of Liquid Metal Droplet Formation in a Co-Flowing Capillary Microfluidic Device
por: Hu, Qingming, et al.
Publicado: (2020)

A Controllable and Integrated Pump-enabled Microfluidic Chip and Its Application in Droplets Generating
por: Zhao, Bei, et al.
Publicado: (2017)

A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR
por: Jalili, Abbas, et al.
Publicado: (2021)

A Self-Priming Microfluidic Chip with Cushion Chambers for Easy Digital PCR
por: Xu, Gangwei, et al.
Publicado: (2021)

Lithography-Free Technology for the Preparation of Digital Microfluidic (DMF) Lab-Chips with Droplet Actuation by Optoelectrowetting (OEW)
por: Doering, Christoph, et al.
Publicado: (2022)

Establishment and Validation of an Integrated Microfluidic Step Emulsification Chip Supporting Droplet Digital Nucleic Acid Analysis
por: Luo, Gangyin, et al.
Publicado: (2023)

Analysis of Phosphatase Activity in a Droplet-Based Microfluidic Chip
por: Vasamsetti, Bala Murali Krishna, et al.
Publicado: (2022)

Numerical Modeling of Physical Cell Trapping in Microfluidic Chips
por: Cardona, Sara, et al.
Publicado: (2023)

Dual-nozzle microfluidic droplet generator
por: Choi, Ji Wook, et al.
Publicado: (2018)

Application of CFD Numerical Simulation Image Imaging Technology in the Study of Droplet Microfluidic Multiphase Flow Characteristics
por: Li, Hao, et al.
Publicado: (2022)

Droplet-based Biosensing for Lab-on-a-Chip, Open Microfluidics Platforms
por: Dak, Piyush, et al.
Publicado: (2016)

The Role of Microfluidics for Organ on Chip Simulations
por: Aziz, Aziz Ur Rehman, et al.
Publicado: (2017)

Micro-injection Molded Droplet Generation System for Digital PCR Application
por: Jo, Daae, et al.
Publicado: (2022)

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator
por: Yao, Junyi, et al.
Publicado: (2019)

Retracted: Application of CFD Numerical Simulation Image Imaging Technology in the Study of Droplet Microfluidic Multiphase Flow Characteristics
por: Imaging, Contrast Media & Molecular
Publicado: (2023)

Microfluidic Droplet-Generation Device with Flexible Walls
por: Yazdanparast, Sajad, et al.
Publicado: (2023)

Transgene Detection by Digital Droplet PCR
por: Moser, Dirk A., et al.
Publicado: (2014)

Droplet Velocity in an Electrowetting on Dielectric Digital Microfluidic Device
por: Nahar, Mun Mun, et al.
Publicado: (2016)

3D-glass molds for facile production of complex droplet microfluidic chips
por: Tovar, Miguel, et al.
Publicado: (2018)

Microfluidic Droplet Digital PCR Is a Powerful Tool for Detection of BRAF and TERT Mutations in Papillary Thyroid Carcinomas
por: Ylli, Dorina, et al.
Publicado: (2019)

Drying in a microfluidic chip: experiments and simulations
por: Fantinel, Paolo, et al.
Publicado: (2017)

A UVM simulation environment for the study, optimization and verification of HL-LHC digital pixel readout chips
por: Marconi, Sara, et al.
Publicado: (2018)

Computational fluid dynamics simulation experimental verification and analysis of droplets deposition behaviour on vibrating pear leaves
por: Cao, Yubin, et al.
Publicado: (2022)

Experimental and numerical study on the transport of droplet aerosols generated by occupants in a fever clinic
por: Zhou, Yu, et al.
Publicado: (2021)

A Chamber-Based Digital PCR Based on a Microfluidic Chip for the Absolute Quantification and Analysis of KRAS Mutation
por: Ren, Jie, et al.
Publicado: (2023)

Electrospun Jets Number and Nanofiber Morphology Effected by Voltage Value: Numerical Simulation and Experimental Verification
por: Liu, Zhi, et al.
Publicado: (2019)

Lithium Niobate Micromachining for the Fabrication of Microfluidic Droplet Generators
por: Bettella, Giacomo, et al.
Publicado: (2017)

Microfluidic Methods for Generation of Submicron Droplets: A Review
por: Huang, Biao, et al.
Publicado: (2023)

Three-dimensional digital microfluidic manipulation of droplets in oil medium
por: Hong, Jiwoo, et al.
Publicado: (2015)

Droplet Transportation through an Orifice on Electrode for Digital Microfluidics Modulations
por: Chu, Ting-Chia, et al.
Publicado: (2021)

Precise Droplet Dispensing in Digital Microfluidics with Dumbbell-Shaped Electrodes
por: Wang, Wei
Publicado: (2022)

Quantification of HEV RNA by Droplet Digital PCR
por: Nicot, Florence, et al.
Publicado: (2016)

Detection of fish allergen by droplet digital PCR
por: Daga, Cinzia, et al.
Publicado: (2019)

Accurate bulk quantitation of droplet digital PCR
por: Sun, Chen, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_67qmtoti39c6vif9ugctogd7ph