Cargando…
Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing
Technological developments in the field of biologically active peptide applications in medicine have increased the need for new methods for peptide delivery. The disadvantage of peptides as drugs is their low biological stability. Recently, great attention has been paid to self-assembling peptides t...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067766/ https://www.ncbi.nlm.nih.gov/pubmed/33917000 http://dx.doi.org/10.3390/ijms22083818 |
_version_ | 1783682879466766336 |
---|---|
author | Sawicka, Justyna Iłowska, Emilia Deptuła, Milena Sosnowski, Paweł Sass, Piotr Czerwiec, Katarzyna Chmielewska, Klaudia Szymańska, Aneta Pietralik-Molińska, Zuzanna Kozak, Maciej Sachadyn, Paweł Pikuła, Michał Rodziewicz-Motowidło, Sylwia |
author_facet | Sawicka, Justyna Iłowska, Emilia Deptuła, Milena Sosnowski, Paweł Sass, Piotr Czerwiec, Katarzyna Chmielewska, Klaudia Szymańska, Aneta Pietralik-Molińska, Zuzanna Kozak, Maciej Sachadyn, Paweł Pikuła, Michał Rodziewicz-Motowidło, Sylwia |
author_sort | Sawicka, Justyna |
collection | PubMed |
description | Technological developments in the field of biologically active peptide applications in medicine have increased the need for new methods for peptide delivery. The disadvantage of peptides as drugs is their low biological stability. Recently, great attention has been paid to self-assembling peptides that can form fibrils. Such a formulation makes bioactive peptides more resistant to enzymatic degradation and druggable. Peptide fibrils can be carriers for peptides with interesting biological activities. These features open up prospects for using the peptide fibrils as long-acting drugs and are a valid alternative to conventional peptidic therapies. In our study, we designed new peptide scaffolds that are a hybrid of three interconnected amino acid sequences and are: pro-regenerative, cleavable by neutrophilic elastase, and fibril-forming. We intended to obtain peptides that are stable in the wound environment and that, when applied, would release a biologically active sequence. Our studies showed that the designed hybrid peptides show a high tendency toward regular fibril formation and are able to release the pro-regenerative sequence. Cytotoxicity studies showed that all the designed peptides were safe, did not cause cytotoxic effects and revealed a pro-regenerative potential in human fibroblast and keratinocyte cell lines. In vivo experiments in a dorsal skin injury model in mice indicated that two tested peptides moderately promote tissue repair in their free form. Our research proves that peptide fibrils can be a druggable form and a scaffold for active peptides. |
format | Online Article Text |
id | pubmed-8067766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80677662021-04-25 Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing Sawicka, Justyna Iłowska, Emilia Deptuła, Milena Sosnowski, Paweł Sass, Piotr Czerwiec, Katarzyna Chmielewska, Klaudia Szymańska, Aneta Pietralik-Molińska, Zuzanna Kozak, Maciej Sachadyn, Paweł Pikuła, Michał Rodziewicz-Motowidło, Sylwia Int J Mol Sci Article Technological developments in the field of biologically active peptide applications in medicine have increased the need for new methods for peptide delivery. The disadvantage of peptides as drugs is their low biological stability. Recently, great attention has been paid to self-assembling peptides that can form fibrils. Such a formulation makes bioactive peptides more resistant to enzymatic degradation and druggable. Peptide fibrils can be carriers for peptides with interesting biological activities. These features open up prospects for using the peptide fibrils as long-acting drugs and are a valid alternative to conventional peptidic therapies. In our study, we designed new peptide scaffolds that are a hybrid of three interconnected amino acid sequences and are: pro-regenerative, cleavable by neutrophilic elastase, and fibril-forming. We intended to obtain peptides that are stable in the wound environment and that, when applied, would release a biologically active sequence. Our studies showed that the designed hybrid peptides show a high tendency toward regular fibril formation and are able to release the pro-regenerative sequence. Cytotoxicity studies showed that all the designed peptides were safe, did not cause cytotoxic effects and revealed a pro-regenerative potential in human fibroblast and keratinocyte cell lines. In vivo experiments in a dorsal skin injury model in mice indicated that two tested peptides moderately promote tissue repair in their free form. Our research proves that peptide fibrils can be a druggable form and a scaffold for active peptides. MDPI 2021-04-07 /pmc/articles/PMC8067766/ /pubmed/33917000 http://dx.doi.org/10.3390/ijms22083818 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sawicka, Justyna Iłowska, Emilia Deptuła, Milena Sosnowski, Paweł Sass, Piotr Czerwiec, Katarzyna Chmielewska, Klaudia Szymańska, Aneta Pietralik-Molińska, Zuzanna Kozak, Maciej Sachadyn, Paweł Pikuła, Michał Rodziewicz-Motowidło, Sylwia Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing |
title | Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing |
title_full | Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing |
title_fullStr | Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing |
title_full_unstemmed | Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing |
title_short | Functionalized Peptide Fibrils as a Scaffold for Active Substances in Wound Healing |
title_sort | functionalized peptide fibrils as a scaffold for active substances in wound healing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067766/ https://www.ncbi.nlm.nih.gov/pubmed/33917000 http://dx.doi.org/10.3390/ijms22083818 |
work_keys_str_mv | AT sawickajustyna functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT iłowskaemilia functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT deptułamilena functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT sosnowskipaweł functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT sasspiotr functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT czerwieckatarzyna functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT chmielewskaklaudia functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT szymanskaaneta functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT pietralikmolinskazuzanna functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT kozakmaciej functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT sachadynpaweł functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT pikułamichał functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing AT rodziewiczmotowidłosylwia functionalizedpeptidefibrilsasascaffoldforactivesubstancesinwoundhealing |