Cargando…

Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis)

Recent studies have explored the seropositivity of Bovine alphaherpesvirus 1 (BoHV-1) in water buffaloes, suggesting the urgency for developing strategies to eradicate the virus involving both cattle and water buffaloes. However, in Europe, the glycoprotein E (gE) deleted marker vaccines against BoH...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrini, Stefano, Martucciello, Alessandra, Grandoni, Francesco, De Matteis, Giovanna, Cappelli, Giovanna, Giammarioli, Monica, Scoccia, Eleonora, Grassi, Carlo, Righi, Cecilia, Fusco, Giovanna, Galiero, Giorgio, Pela, Michela, De Mia, Gian Mario, De Carlo, Esterina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067792/
https://www.ncbi.nlm.nih.gov/pubmed/33917160
http://dx.doi.org/10.3390/vaccines9040355
_version_ 1783682885585207296
author Petrini, Stefano
Martucciello, Alessandra
Grandoni, Francesco
De Matteis, Giovanna
Cappelli, Giovanna
Giammarioli, Monica
Scoccia, Eleonora
Grassi, Carlo
Righi, Cecilia
Fusco, Giovanna
Galiero, Giorgio
Pela, Michela
De Mia, Gian Mario
De Carlo, Esterina
author_facet Petrini, Stefano
Martucciello, Alessandra
Grandoni, Francesco
De Matteis, Giovanna
Cappelli, Giovanna
Giammarioli, Monica
Scoccia, Eleonora
Grassi, Carlo
Righi, Cecilia
Fusco, Giovanna
Galiero, Giorgio
Pela, Michela
De Mia, Gian Mario
De Carlo, Esterina
author_sort Petrini, Stefano
collection PubMed
description Recent studies have explored the seropositivity of Bovine alphaherpesvirus 1 (BoHV-1) in water buffaloes, suggesting the urgency for developing strategies to eradicate the virus involving both cattle and water buffaloes. However, in Europe, the glycoprotein E (gE) deleted marker vaccines against BoHV-1 are commercially available only for the cattle industry. This study, for the first time, evaluated the safety and efficacy of a commercial inactivated gE-deleted marker vaccine in water buffalo. Five animals devoid of BoHV-1-neutralizing antibodies were vaccinated via intramuscular route. Five additional animals served as an unvaccinated control group. Sixty days after the first immunization, all animals were experimentally infected with a virulent BoHV-1via intranasal route. A detectable BoHV-1-humoral immune response was observed in the vaccinated group on post-vaccination day 30, whereas the antibodies appeared on post-challenge day 10 in the control group. Moreover, the vaccinated animals neither show viral shedding nor clinical signs compared to the control upon challenge. However, post-challenge, the BoHV-1-specific humoral and cell-mediated immune responses were significantly more increased in vaccinated animals than the control animals. Overall, the present study provides evidence of both the safety and efficacy of an inactivated gE-deleted marker vaccine against BoHV-1 in water buffaloes.
format Online
Article
Text
id pubmed-8067792
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80677922021-04-25 Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis) Petrini, Stefano Martucciello, Alessandra Grandoni, Francesco De Matteis, Giovanna Cappelli, Giovanna Giammarioli, Monica Scoccia, Eleonora Grassi, Carlo Righi, Cecilia Fusco, Giovanna Galiero, Giorgio Pela, Michela De Mia, Gian Mario De Carlo, Esterina Vaccines (Basel) Article Recent studies have explored the seropositivity of Bovine alphaherpesvirus 1 (BoHV-1) in water buffaloes, suggesting the urgency for developing strategies to eradicate the virus involving both cattle and water buffaloes. However, in Europe, the glycoprotein E (gE) deleted marker vaccines against BoHV-1 are commercially available only for the cattle industry. This study, for the first time, evaluated the safety and efficacy of a commercial inactivated gE-deleted marker vaccine in water buffalo. Five animals devoid of BoHV-1-neutralizing antibodies were vaccinated via intramuscular route. Five additional animals served as an unvaccinated control group. Sixty days after the first immunization, all animals were experimentally infected with a virulent BoHV-1via intranasal route. A detectable BoHV-1-humoral immune response was observed in the vaccinated group on post-vaccination day 30, whereas the antibodies appeared on post-challenge day 10 in the control group. Moreover, the vaccinated animals neither show viral shedding nor clinical signs compared to the control upon challenge. However, post-challenge, the BoHV-1-specific humoral and cell-mediated immune responses were significantly more increased in vaccinated animals than the control animals. Overall, the present study provides evidence of both the safety and efficacy of an inactivated gE-deleted marker vaccine against BoHV-1 in water buffaloes. MDPI 2021-04-07 /pmc/articles/PMC8067792/ /pubmed/33917160 http://dx.doi.org/10.3390/vaccines9040355 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Petrini, Stefano
Martucciello, Alessandra
Grandoni, Francesco
De Matteis, Giovanna
Cappelli, Giovanna
Giammarioli, Monica
Scoccia, Eleonora
Grassi, Carlo
Righi, Cecilia
Fusco, Giovanna
Galiero, Giorgio
Pela, Michela
De Mia, Gian Mario
De Carlo, Esterina
Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis)
title Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis)
title_full Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis)
title_fullStr Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis)
title_full_unstemmed Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis)
title_short Evaluation of Safety and Efficacy of an Inactivated Marker Vaccine against Bovine alphaherpesvirus 1 (BoHV-1) in Water Buffalo (Bubalus bubalis)
title_sort evaluation of safety and efficacy of an inactivated marker vaccine against bovine alphaherpesvirus 1 (bohv-1) in water buffalo (bubalus bubalis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067792/
https://www.ncbi.nlm.nih.gov/pubmed/33917160
http://dx.doi.org/10.3390/vaccines9040355
work_keys_str_mv AT petrinistefano evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT martuccielloalessandra evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT grandonifrancesco evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT dematteisgiovanna evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT cappelligiovanna evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT giammariolimonica evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT scocciaeleonora evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT grassicarlo evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT righicecilia evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT fuscogiovanna evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT galierogiorgio evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT pelamichela evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT demiagianmario evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis
AT decarloesterina evaluationofsafetyandefficacyofaninactivatedmarkervaccineagainstbovinealphaherpesvirus1bohv1inwaterbuffalobubalusbubalis