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Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however...

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Autores principales: Tarmizi, Auni Aqilah Ahmad, Wagiran, Alina, Mohd Salleh, Faezah, Chua, Lee Suan, Abdullah, Farah Izana, Hasham, Rosnani, Binte Mostafiz, Suraiya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067811/
https://www.ncbi.nlm.nih.gov/pubmed/33917172
http://dx.doi.org/10.3390/plants10040717
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author Tarmizi, Auni Aqilah Ahmad
Wagiran, Alina
Mohd Salleh, Faezah
Chua, Lee Suan
Abdullah, Farah Izana
Hasham, Rosnani
Binte Mostafiz, Suraiya
author_facet Tarmizi, Auni Aqilah Ahmad
Wagiran, Alina
Mohd Salleh, Faezah
Chua, Lee Suan
Abdullah, Farah Izana
Hasham, Rosnani
Binte Mostafiz, Suraiya
author_sort Tarmizi, Auni Aqilah Ahmad
collection PubMed
description Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L. pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products.
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spelling pubmed-80678112021-04-25 Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC Tarmizi, Auni Aqilah Ahmad Wagiran, Alina Mohd Salleh, Faezah Chua, Lee Suan Abdullah, Farah Izana Hasham, Rosnani Binte Mostafiz, Suraiya Plants (Basel) Article Labisia pumila is a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use of Labisia herbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2 and rbcL) for L. pumila var. alata and pumila. The DNA barcode that was generated was then used to identify species of Labisia pumila in herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products. MDPI 2021-04-07 /pmc/articles/PMC8067811/ /pubmed/33917172 http://dx.doi.org/10.3390/plants10040717 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tarmizi, Auni Aqilah Ahmad
Wagiran, Alina
Mohd Salleh, Faezah
Chua, Lee Suan
Abdullah, Farah Izana
Hasham, Rosnani
Binte Mostafiz, Suraiya
Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC
title Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC
title_full Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC
title_fullStr Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC
title_full_unstemmed Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC
title_short Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC
title_sort integrated approach for species identification and quality analysis for labisia pumila using dna barcoding and hplc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067811/
https://www.ncbi.nlm.nih.gov/pubmed/33917172
http://dx.doi.org/10.3390/plants10040717
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