Cargando…

Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions

A study was carried out on the effect of the root endophytic fungus Pochonia chlamydosporia on plant systemic signal of defense related genes during fungal or nematode parasitism. Different biotic stress factors were examined, inoculating roots of dicot and monocot hosts with the endophyte, and meas...

Descripción completa

Detalles Bibliográficos
Autores principales: Tolba, Shimaa R. T., Rosso, Laura C., Pentimone, Isabella, Colagiero, Mariantonietta, Moustafa, Mahmoud M. A., Elshawaf, Ibrahim I. S., Bubici, Giovanni, Prigigallo, Maria Isabella, Ciancio, Aurelio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068004/
https://www.ncbi.nlm.nih.gov/pubmed/33917204
http://dx.doi.org/10.3390/plants10040718
_version_ 1783682934841016320
author Tolba, Shimaa R. T.
Rosso, Laura C.
Pentimone, Isabella
Colagiero, Mariantonietta
Moustafa, Mahmoud M. A.
Elshawaf, Ibrahim I. S.
Bubici, Giovanni
Prigigallo, Maria Isabella
Ciancio, Aurelio
author_facet Tolba, Shimaa R. T.
Rosso, Laura C.
Pentimone, Isabella
Colagiero, Mariantonietta
Moustafa, Mahmoud M. A.
Elshawaf, Ibrahim I. S.
Bubici, Giovanni
Prigigallo, Maria Isabella
Ciancio, Aurelio
author_sort Tolba, Shimaa R. T.
collection PubMed
description A study was carried out on the effect of the root endophytic fungus Pochonia chlamydosporia on plant systemic signal of defense related genes during fungal or nematode parasitism. Different biotic stress factors were examined, inoculating roots of dicot and monocot hosts with the endophyte, and measuring the expression of defense genes in leaves. A first greenhouse assay was carried out on expression of PAL, PIN II, PR1 and LOX D in leaves of tomato cv Tondino inoculated with Phytophthora infestans (CBS 120920), inoculating or not the roots of infected plants with P. chlamydosporia DSM 26985. In a second assay, plants of banana (Musa acuminata cv Grand Naine) were artificially infected with Fusarium oxysporum f. sp. cubense Tropical race 4 (TR4) and inoculated or not with DSM 26985. In a further experiment, banana plants were inoculated or not with P. chlamydosporia plus juveniles of the root knot nematode (RKN) Meloidogyne incognita. A similar assay was also carried out in vitro with adults and juveniles of the lesion nematode Pratylenchus goodeyi. Differential expression of the defense genes examined was observed for all plant-stress associations, indicative of early, upward systemic signals induced by the endophyte. Changes in expression profiles included a 5-fold down-regulation of PIN II at 2 dai in leaves of tomato plants treated with P. infestans and/or P. chlamydosporia, and the up-regulation of PAL by the endophyte alone, at 2 and 7 dai. In the TR4 assay, PR1 was significantly up-regulated at 7 dai in banana leaves, but only in the P. chlamydosporia treated plants. At 10 dai, PIN II expression was significantly higher in leaves of plants inoculated only with TR4. The banana-RKN assay showed a PR1 expression significantly higher than controls at 4 and 7 dai in plants inoculated with P. chlamydosporia alone, and a down-regulation at 4 dai in leaves of plants also inoculated with RKN, with a PR1 differential up-regulation at 10 dai. Pratylenchus goodeyi down-regulated PIN at 21 dai, with or without the endophyte, as well as PAL but only in presence of P. chlamydosporia. When inoculated alone, the endophyte up-regulated PR1 and LOX. The gene expression patterns observed in leaves suggest specific and time-dependent relationships linking host plants and P. chlamydosporia in presence of biotic stress factors, functional to a systemic, although complex, activation of defense genes.
format Online
Article
Text
id pubmed-8068004
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80680042021-04-25 Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions Tolba, Shimaa R. T. Rosso, Laura C. Pentimone, Isabella Colagiero, Mariantonietta Moustafa, Mahmoud M. A. Elshawaf, Ibrahim I. S. Bubici, Giovanni Prigigallo, Maria Isabella Ciancio, Aurelio Plants (Basel) Article A study was carried out on the effect of the root endophytic fungus Pochonia chlamydosporia on plant systemic signal of defense related genes during fungal or nematode parasitism. Different biotic stress factors were examined, inoculating roots of dicot and monocot hosts with the endophyte, and measuring the expression of defense genes in leaves. A first greenhouse assay was carried out on expression of PAL, PIN II, PR1 and LOX D in leaves of tomato cv Tondino inoculated with Phytophthora infestans (CBS 120920), inoculating or not the roots of infected plants with P. chlamydosporia DSM 26985. In a second assay, plants of banana (Musa acuminata cv Grand Naine) were artificially infected with Fusarium oxysporum f. sp. cubense Tropical race 4 (TR4) and inoculated or not with DSM 26985. In a further experiment, banana plants were inoculated or not with P. chlamydosporia plus juveniles of the root knot nematode (RKN) Meloidogyne incognita. A similar assay was also carried out in vitro with adults and juveniles of the lesion nematode Pratylenchus goodeyi. Differential expression of the defense genes examined was observed for all plant-stress associations, indicative of early, upward systemic signals induced by the endophyte. Changes in expression profiles included a 5-fold down-regulation of PIN II at 2 dai in leaves of tomato plants treated with P. infestans and/or P. chlamydosporia, and the up-regulation of PAL by the endophyte alone, at 2 and 7 dai. In the TR4 assay, PR1 was significantly up-regulated at 7 dai in banana leaves, but only in the P. chlamydosporia treated plants. At 10 dai, PIN II expression was significantly higher in leaves of plants inoculated only with TR4. The banana-RKN assay showed a PR1 expression significantly higher than controls at 4 and 7 dai in plants inoculated with P. chlamydosporia alone, and a down-regulation at 4 dai in leaves of plants also inoculated with RKN, with a PR1 differential up-regulation at 10 dai. Pratylenchus goodeyi down-regulated PIN at 21 dai, with or without the endophyte, as well as PAL but only in presence of P. chlamydosporia. When inoculated alone, the endophyte up-regulated PR1 and LOX. The gene expression patterns observed in leaves suggest specific and time-dependent relationships linking host plants and P. chlamydosporia in presence of biotic stress factors, functional to a systemic, although complex, activation of defense genes. MDPI 2021-04-07 /pmc/articles/PMC8068004/ /pubmed/33917204 http://dx.doi.org/10.3390/plants10040718 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tolba, Shimaa R. T.
Rosso, Laura C.
Pentimone, Isabella
Colagiero, Mariantonietta
Moustafa, Mahmoud M. A.
Elshawaf, Ibrahim I. S.
Bubici, Giovanni
Prigigallo, Maria Isabella
Ciancio, Aurelio
Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions
title Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions
title_full Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions
title_fullStr Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions
title_full_unstemmed Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions
title_short Root Endophytism by Pochonia chlamydosporia Affects Defense-Gene Expression in Leaves of Monocot and Dicot Hosts under Multiple Biotic Interactions
title_sort root endophytism by pochonia chlamydosporia affects defense-gene expression in leaves of monocot and dicot hosts under multiple biotic interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068004/
https://www.ncbi.nlm.nih.gov/pubmed/33917204
http://dx.doi.org/10.3390/plants10040718
work_keys_str_mv AT tolbashimaart rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions
AT rossolaurac rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions
AT pentimoneisabella rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions
AT colagieromariantonietta rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions
AT moustafamahmoudma rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions
AT elshawafibrahimis rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions
AT bubicigiovanni rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions
AT prigigallomariaisabella rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions
AT ciancioaurelio rootendophytismbypochoniachlamydosporiaaffectsdefensegeneexpressioninleavesofmonocotanddicothostsundermultiplebioticinteractions