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Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry

Pathological biopsy protocols require tissue marking dye (TMD) for orientation. In some cases (e.g., close margin), additional immunohistochemical analyses can be necessary. Therefore, the correlation between the applied TMD during macroscopy and the examined TMD during microscopy is crucial for the...

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Autores principales: Kiefer, Selina, Huber, Julia, Füllgraf, Hannah, Sörensen, Kristin, Csanadi, Agnes, Stillger, Maren Nicole, Werner, Martin, Schaefer, Hans-Eckart, Bronsert, Peter, Aumann, Konrad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068012/
https://www.ncbi.nlm.nih.gov/pubmed/33917667
http://dx.doi.org/10.3390/cells10040835
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author Kiefer, Selina
Huber, Julia
Füllgraf, Hannah
Sörensen, Kristin
Csanadi, Agnes
Stillger, Maren Nicole
Werner, Martin
Schaefer, Hans-Eckart
Bronsert, Peter
Aumann, Konrad
author_facet Kiefer, Selina
Huber, Julia
Füllgraf, Hannah
Sörensen, Kristin
Csanadi, Agnes
Stillger, Maren Nicole
Werner, Martin
Schaefer, Hans-Eckart
Bronsert, Peter
Aumann, Konrad
author_sort Kiefer, Selina
collection PubMed
description Pathological biopsy protocols require tissue marking dye (TMD) for orientation. In some cases (e.g., close margin), additional immunohistochemical analyses can be necessary. Therefore, the correlation between the applied TMD during macroscopy and the examined TMD during microscopy is crucial for the correct orientation, the residual tumour status and the subsequent therapeutic regime. In this context, our group observed colour changes during routine immunohistochemistry. Tissue specimens were marked with various TMD and processed by two different methods. TMD (blue, red, black, yellow and green) obtained from three different providers (A, B and C, and Whiteout/Tipp-Ex(®)) were used. Immunohistochemistry was performed manually via stepwise omission of reagents to identify the colour changing mechanism. Blue colour from provider A changed during immunohistochemistry into black, when 3,3′-Diaminobenzidine-tetrahydrochloride-dihydrate (DAB) and H(2)O(2) was applied as an immunoperoxidase-based terminal colour signal. No other applied reagents, nor tissue texture or processing showed any influence on the colour. The remaining colours from provider A and the other colours did not show any changes during immunohistochemistry. Our results demonstrate an interesting and important pitfall in routine immunohistochemistry-based diagnostics that pathologists should be aware of. Furthermore, the chemical rationale behind the observed misleading colour change is discussed.
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spelling pubmed-80680122021-04-25 Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry Kiefer, Selina Huber, Julia Füllgraf, Hannah Sörensen, Kristin Csanadi, Agnes Stillger, Maren Nicole Werner, Martin Schaefer, Hans-Eckart Bronsert, Peter Aumann, Konrad Cells Communication Pathological biopsy protocols require tissue marking dye (TMD) for orientation. In some cases (e.g., close margin), additional immunohistochemical analyses can be necessary. Therefore, the correlation between the applied TMD during macroscopy and the examined TMD during microscopy is crucial for the correct orientation, the residual tumour status and the subsequent therapeutic regime. In this context, our group observed colour changes during routine immunohistochemistry. Tissue specimens were marked with various TMD and processed by two different methods. TMD (blue, red, black, yellow and green) obtained from three different providers (A, B and C, and Whiteout/Tipp-Ex(®)) were used. Immunohistochemistry was performed manually via stepwise omission of reagents to identify the colour changing mechanism. Blue colour from provider A changed during immunohistochemistry into black, when 3,3′-Diaminobenzidine-tetrahydrochloride-dihydrate (DAB) and H(2)O(2) was applied as an immunoperoxidase-based terminal colour signal. No other applied reagents, nor tissue texture or processing showed any influence on the colour. The remaining colours from provider A and the other colours did not show any changes during immunohistochemistry. Our results demonstrate an interesting and important pitfall in routine immunohistochemistry-based diagnostics that pathologists should be aware of. Furthermore, the chemical rationale behind the observed misleading colour change is discussed. MDPI 2021-04-08 /pmc/articles/PMC8068012/ /pubmed/33917667 http://dx.doi.org/10.3390/cells10040835 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kiefer, Selina
Huber, Julia
Füllgraf, Hannah
Sörensen, Kristin
Csanadi, Agnes
Stillger, Maren Nicole
Werner, Martin
Schaefer, Hans-Eckart
Bronsert, Peter
Aumann, Konrad
Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry
title Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry
title_full Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry
title_fullStr Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry
title_full_unstemmed Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry
title_short Alteration of Tissue Marking Dyes Depends on Used Chromogen during Immunohistochemistry
title_sort alteration of tissue marking dyes depends on used chromogen during immunohistochemistry
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068012/
https://www.ncbi.nlm.nih.gov/pubmed/33917667
http://dx.doi.org/10.3390/cells10040835
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