Cargando…
Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment
The development of antibacterial materials has great importance in avoiding bacterial contamination and the risk of infection for implantable biomaterials. An antibacterial thin film coating on the surface via chemical bonding is a promising technique to keep native bulk material properties unchange...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068050/ https://www.ncbi.nlm.nih.gov/pubmed/33917700 http://dx.doi.org/10.3390/polym13081201 |
_version_ | 1783682945705312256 |
---|---|
author | Ozaltin, Kadir Di Martino, Antonio Capakova, Zdenka Lehocky, Marian Humpolicek, Petr Saha, Tomas Vesela, Daniela Mozetic, Miran Saha, Petr |
author_facet | Ozaltin, Kadir Di Martino, Antonio Capakova, Zdenka Lehocky, Marian Humpolicek, Petr Saha, Tomas Vesela, Daniela Mozetic, Miran Saha, Petr |
author_sort | Ozaltin, Kadir |
collection | PubMed |
description | The development of antibacterial materials has great importance in avoiding bacterial contamination and the risk of infection for implantable biomaterials. An antibacterial thin film coating on the surface via chemical bonding is a promising technique to keep native bulk material properties unchanged. However, most of the polymeric materials are chemically inert and highly hydrophobic, which makes chemical agent coating challenging Herein, immobilization of chlorhexidine, a broad-spectrum bactericidal cationic compound, onto the polylactic acid surface was performed in a multistep physicochemical method. Direct current plasma was used for surface functionalization, followed by carbodiimide chemistry to link the coupling reagents of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC) and N-Hydroxysuccinimide (NHs) to create a free bonding site to anchor the chlorhexidine. Surface characterizations were performed by water contact angle test, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). The antibacterial activity was tested using Staphylococcus aureus and Escherichia coli. Finally, in vitro cytocompatibility of the samples was studied using primary mouse embryonic fibroblast cells. It was found that all samples were cytocompatible and the best antibacterial performance observed was the Chlorhexidine immobilized sample after NHs activation. |
format | Online Article Text |
id | pubmed-8068050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80680502021-04-25 Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment Ozaltin, Kadir Di Martino, Antonio Capakova, Zdenka Lehocky, Marian Humpolicek, Petr Saha, Tomas Vesela, Daniela Mozetic, Miran Saha, Petr Polymers (Basel) Article The development of antibacterial materials has great importance in avoiding bacterial contamination and the risk of infection for implantable biomaterials. An antibacterial thin film coating on the surface via chemical bonding is a promising technique to keep native bulk material properties unchanged. However, most of the polymeric materials are chemically inert and highly hydrophobic, which makes chemical agent coating challenging Herein, immobilization of chlorhexidine, a broad-spectrum bactericidal cationic compound, onto the polylactic acid surface was performed in a multistep physicochemical method. Direct current plasma was used for surface functionalization, followed by carbodiimide chemistry to link the coupling reagents of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC) and N-Hydroxysuccinimide (NHs) to create a free bonding site to anchor the chlorhexidine. Surface characterizations were performed by water contact angle test, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). The antibacterial activity was tested using Staphylococcus aureus and Escherichia coli. Finally, in vitro cytocompatibility of the samples was studied using primary mouse embryonic fibroblast cells. It was found that all samples were cytocompatible and the best antibacterial performance observed was the Chlorhexidine immobilized sample after NHs activation. MDPI 2021-04-08 /pmc/articles/PMC8068050/ /pubmed/33917700 http://dx.doi.org/10.3390/polym13081201 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ozaltin, Kadir Di Martino, Antonio Capakova, Zdenka Lehocky, Marian Humpolicek, Petr Saha, Tomas Vesela, Daniela Mozetic, Miran Saha, Petr Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment |
title | Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment |
title_full | Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment |
title_fullStr | Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment |
title_full_unstemmed | Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment |
title_short | Plasma Mediated Chlorhexidine Immobilization onto Polylactic Acid Surface via Carbodiimide Chemistry: Antibacterial and Cytocompatibility Assessment |
title_sort | plasma mediated chlorhexidine immobilization onto polylactic acid surface via carbodiimide chemistry: antibacterial and cytocompatibility assessment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068050/ https://www.ncbi.nlm.nih.gov/pubmed/33917700 http://dx.doi.org/10.3390/polym13081201 |
work_keys_str_mv | AT ozaltinkadir plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment AT dimartinoantonio plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment AT capakovazdenka plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment AT lehockymarian plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment AT humpolicekpetr plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment AT sahatomas plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment AT veseladaniela plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment AT mozeticmiran plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment AT sahapetr plasmamediatedchlorhexidineimmobilizationontopolylacticacidsurfaceviacarbodiimidechemistryantibacterialandcytocompatibilityassessment |