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Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence

The decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response is not yet deeply understood. Since macrophages play a key...

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Autores principales: Cicuéndez, Mónica, Casarrubios, Laura, Feito, María José, Madarieta, Iratxe, Garcia-Urkia, Nerea, Murua, Olatz, Olalde, Beatriz, Briz, Nerea, Diez-Orejas, Rosalía, Portolés, María Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068109/
https://www.ncbi.nlm.nih.gov/pubmed/33917732
http://dx.doi.org/10.3390/ijms22083847
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author Cicuéndez, Mónica
Casarrubios, Laura
Feito, María José
Madarieta, Iratxe
Garcia-Urkia, Nerea
Murua, Olatz
Olalde, Beatriz
Briz, Nerea
Diez-Orejas, Rosalía
Portolés, María Teresa
author_facet Cicuéndez, Mónica
Casarrubios, Laura
Feito, María José
Madarieta, Iratxe
Garcia-Urkia, Nerea
Murua, Olatz
Olalde, Beatriz
Briz, Nerea
Diez-Orejas, Rosalía
Portolés, María Teresa
author_sort Cicuéndez, Mónica
collection PubMed
description The decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response is not yet deeply understood. Since macrophages play a key role in the inflammation/healing processes due to their high functional plasticity between M1 and M2 phenotypes, the evaluation of their response to decellularized ECM is mandatory. It is also necessary to analyze the immunocompetence of macrophages after contact with decellularized ECM materials to assess their functional role in a possible infection scenario. In this work, we studied the effect of four decellularized adipose matrices (DAMs) obtained from human and porcine AT by enzymatic or chemical methods on macrophage phenotypes and fungal phagocytosis. First, a thorough biochemical characterization of these biomaterials by quantification of remnant DNA, lipids, and proteins was performed, thus indicating the efficiency and reliability of both methods. The proteomic analysis evidenced that some proteins are differentially preserved depending on both the AT origin and the decellularization method employed. After exposure to the four DAMs, specific markers of M1 proinflammatory and M2 anti-inflammatory macrophages were analyzed. Porcine DAMs favor the M2 phenotype, independently of the decellularization method employed. Finally, a sensitive fungal phagocytosis assay allowed us to relate the macrophage phagocytosis capability with specific proteins differentially preserved in certain DAMs. The results obtained in this study highlight the close relationship between the ECM biochemical composition and the macrophage’s functional role.
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spelling pubmed-80681092021-04-25 Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence Cicuéndez, Mónica Casarrubios, Laura Feito, María José Madarieta, Iratxe Garcia-Urkia, Nerea Murua, Olatz Olalde, Beatriz Briz, Nerea Diez-Orejas, Rosalía Portolés, María Teresa Int J Mol Sci Article The decellularized extracellular matrix (ECM) obtained from human and porcine adipose tissue (AT) is currently used to prepare regenerative medicine bio-scaffolds. However, the influence of these natural biomaterials on host immune response is not yet deeply understood. Since macrophages play a key role in the inflammation/healing processes due to their high functional plasticity between M1 and M2 phenotypes, the evaluation of their response to decellularized ECM is mandatory. It is also necessary to analyze the immunocompetence of macrophages after contact with decellularized ECM materials to assess their functional role in a possible infection scenario. In this work, we studied the effect of four decellularized adipose matrices (DAMs) obtained from human and porcine AT by enzymatic or chemical methods on macrophage phenotypes and fungal phagocytosis. First, a thorough biochemical characterization of these biomaterials by quantification of remnant DNA, lipids, and proteins was performed, thus indicating the efficiency and reliability of both methods. The proteomic analysis evidenced that some proteins are differentially preserved depending on both the AT origin and the decellularization method employed. After exposure to the four DAMs, specific markers of M1 proinflammatory and M2 anti-inflammatory macrophages were analyzed. Porcine DAMs favor the M2 phenotype, independently of the decellularization method employed. Finally, a sensitive fungal phagocytosis assay allowed us to relate the macrophage phagocytosis capability with specific proteins differentially preserved in certain DAMs. The results obtained in this study highlight the close relationship between the ECM biochemical composition and the macrophage’s functional role. MDPI 2021-04-08 /pmc/articles/PMC8068109/ /pubmed/33917732 http://dx.doi.org/10.3390/ijms22083847 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cicuéndez, Mónica
Casarrubios, Laura
Feito, María José
Madarieta, Iratxe
Garcia-Urkia, Nerea
Murua, Olatz
Olalde, Beatriz
Briz, Nerea
Diez-Orejas, Rosalía
Portolés, María Teresa
Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_full Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_fullStr Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_full_unstemmed Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_short Effects of Human and Porcine Adipose Extracellular Matrices Decellularized by Enzymatic or Chemical Methods on Macrophage Polarization and Immunocompetence
title_sort effects of human and porcine adipose extracellular matrices decellularized by enzymatic or chemical methods on macrophage polarization and immunocompetence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068109/
https://www.ncbi.nlm.nih.gov/pubmed/33917732
http://dx.doi.org/10.3390/ijms22083847
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