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NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells

The NF-κB signaling pathway is crucial during development and inflammatory processes. We have previously shown that NF-κB activation induces dedifferentiation of astrocytes into neural progenitor cells (NPCs). Here, we provide evidence  that the NF-κB pathway plays also a fundamental role during the...

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Autores principales: Birck, Cindy, Ginolhac, Aurélien, Pavlou, Maria Angeliki S., Michelucci, Alessandro, Heuschling, Paul, Grandbarbe, Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068246/
https://www.ncbi.nlm.nih.gov/pubmed/33917855
http://dx.doi.org/10.3390/cells10040840
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author Birck, Cindy
Ginolhac, Aurélien
Pavlou, Maria Angeliki S.
Michelucci, Alessandro
Heuschling, Paul
Grandbarbe, Luc
author_facet Birck, Cindy
Ginolhac, Aurélien
Pavlou, Maria Angeliki S.
Michelucci, Alessandro
Heuschling, Paul
Grandbarbe, Luc
author_sort Birck, Cindy
collection PubMed
description The NF-κB signaling pathway is crucial during development and inflammatory processes. We have previously shown that NF-κB activation induces dedifferentiation of astrocytes into neural progenitor cells (NPCs). Here, we provide evidence  that the NF-κB pathway plays also a fundamental role during the differentiation of NPCs into astrocytes. First, we show that the NF-κB pathway is essential to initiate astrocytic differentiation as its early inhibition induces NPC apoptosis and impedes their differentiation. Second, we demonstrate that persistent NF-κB activation affects NPC-derived astrocyte differentiation. Tumor necrosis factor (TNF)-treated NPCs show NF-κB activation, maintain their multipotential and proliferation properties, display persistent expression of immature markers and inhibit astrocyte markers. Third, we analyze the effect of  NF-κB activation on the main known astrocytic differentiation pathways, such as NOTCH and JAK-STAT. Our findings suggest that the NF-κB pathway plays a dual fundamental role during NPC differentiation into astrocytes: it promotes astrocyte specification, but its persistent activation impedes their differentiation.
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spelling pubmed-80682462021-04-25 NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells Birck, Cindy Ginolhac, Aurélien Pavlou, Maria Angeliki S. Michelucci, Alessandro Heuschling, Paul Grandbarbe, Luc Cells Article The NF-κB signaling pathway is crucial during development and inflammatory processes. We have previously shown that NF-κB activation induces dedifferentiation of astrocytes into neural progenitor cells (NPCs). Here, we provide evidence  that the NF-κB pathway plays also a fundamental role during the differentiation of NPCs into astrocytes. First, we show that the NF-κB pathway is essential to initiate astrocytic differentiation as its early inhibition induces NPC apoptosis and impedes their differentiation. Second, we demonstrate that persistent NF-κB activation affects NPC-derived astrocyte differentiation. Tumor necrosis factor (TNF)-treated NPCs show NF-κB activation, maintain their multipotential and proliferation properties, display persistent expression of immature markers and inhibit astrocyte markers. Third, we analyze the effect of  NF-κB activation on the main known astrocytic differentiation pathways, such as NOTCH and JAK-STAT. Our findings suggest that the NF-κB pathway plays a dual fundamental role during NPC differentiation into astrocytes: it promotes astrocyte specification, but its persistent activation impedes their differentiation. MDPI 2021-04-08 /pmc/articles/PMC8068246/ /pubmed/33917855 http://dx.doi.org/10.3390/cells10040840 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Birck, Cindy
Ginolhac, Aurélien
Pavlou, Maria Angeliki S.
Michelucci, Alessandro
Heuschling, Paul
Grandbarbe, Luc
NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells
title NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells
title_full NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells
title_fullStr NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells
title_full_unstemmed NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells
title_short NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells
title_sort nf-κb and tnf affect the astrocytic differentiation from neural stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068246/
https://www.ncbi.nlm.nih.gov/pubmed/33917855
http://dx.doi.org/10.3390/cells10040840
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