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NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells
The NF-κB signaling pathway is crucial during development and inflammatory processes. We have previously shown that NF-κB activation induces dedifferentiation of astrocytes into neural progenitor cells (NPCs). Here, we provide evidence that the NF-κB pathway plays also a fundamental role during the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068246/ https://www.ncbi.nlm.nih.gov/pubmed/33917855 http://dx.doi.org/10.3390/cells10040840 |
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author | Birck, Cindy Ginolhac, Aurélien Pavlou, Maria Angeliki S. Michelucci, Alessandro Heuschling, Paul Grandbarbe, Luc |
author_facet | Birck, Cindy Ginolhac, Aurélien Pavlou, Maria Angeliki S. Michelucci, Alessandro Heuschling, Paul Grandbarbe, Luc |
author_sort | Birck, Cindy |
collection | PubMed |
description | The NF-κB signaling pathway is crucial during development and inflammatory processes. We have previously shown that NF-κB activation induces dedifferentiation of astrocytes into neural progenitor cells (NPCs). Here, we provide evidence that the NF-κB pathway plays also a fundamental role during the differentiation of NPCs into astrocytes. First, we show that the NF-κB pathway is essential to initiate astrocytic differentiation as its early inhibition induces NPC apoptosis and impedes their differentiation. Second, we demonstrate that persistent NF-κB activation affects NPC-derived astrocyte differentiation. Tumor necrosis factor (TNF)-treated NPCs show NF-κB activation, maintain their multipotential and proliferation properties, display persistent expression of immature markers and inhibit astrocyte markers. Third, we analyze the effect of NF-κB activation on the main known astrocytic differentiation pathways, such as NOTCH and JAK-STAT. Our findings suggest that the NF-κB pathway plays a dual fundamental role during NPC differentiation into astrocytes: it promotes astrocyte specification, but its persistent activation impedes their differentiation. |
format | Online Article Text |
id | pubmed-8068246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80682462021-04-25 NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells Birck, Cindy Ginolhac, Aurélien Pavlou, Maria Angeliki S. Michelucci, Alessandro Heuschling, Paul Grandbarbe, Luc Cells Article The NF-κB signaling pathway is crucial during development and inflammatory processes. We have previously shown that NF-κB activation induces dedifferentiation of astrocytes into neural progenitor cells (NPCs). Here, we provide evidence that the NF-κB pathway plays also a fundamental role during the differentiation of NPCs into astrocytes. First, we show that the NF-κB pathway is essential to initiate astrocytic differentiation as its early inhibition induces NPC apoptosis and impedes their differentiation. Second, we demonstrate that persistent NF-κB activation affects NPC-derived astrocyte differentiation. Tumor necrosis factor (TNF)-treated NPCs show NF-κB activation, maintain their multipotential and proliferation properties, display persistent expression of immature markers and inhibit astrocyte markers. Third, we analyze the effect of NF-κB activation on the main known astrocytic differentiation pathways, such as NOTCH and JAK-STAT. Our findings suggest that the NF-κB pathway plays a dual fundamental role during NPC differentiation into astrocytes: it promotes astrocyte specification, but its persistent activation impedes their differentiation. MDPI 2021-04-08 /pmc/articles/PMC8068246/ /pubmed/33917855 http://dx.doi.org/10.3390/cells10040840 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Birck, Cindy Ginolhac, Aurélien Pavlou, Maria Angeliki S. Michelucci, Alessandro Heuschling, Paul Grandbarbe, Luc NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells |
title | NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells |
title_full | NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells |
title_fullStr | NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells |
title_full_unstemmed | NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells |
title_short | NF-κB and TNF Affect the Astrocytic Differentiation from Neural Stem Cells |
title_sort | nf-κb and tnf affect the astrocytic differentiation from neural stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068246/ https://www.ncbi.nlm.nih.gov/pubmed/33917855 http://dx.doi.org/10.3390/cells10040840 |
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