Cargando…

IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice

Inflammation has a dual effect: it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of IL-1R1 in liver regeneration (LR) after partial hepatectomy (PH) in aged mice. The wild-type (WT, n = 36) and the IL-1R1 knockout (KO, n = 36) 24-m...

Descripción completa

Detalles Bibliográficos
Autores principales: LI, Deming, WANG, Ze, ZHANG, Chunyan, XU, Cunshuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific and Technological Research Council of Turkey 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068764/
https://www.ncbi.nlm.nih.gov/pubmed/33907503
http://dx.doi.org/10.3906/biy-2010-51
_version_ 1783683079060062208
author LI, Deming
WANG, Ze
ZHANG, Chunyan
XU, Cunshuan
author_facet LI, Deming
WANG, Ze
ZHANG, Chunyan
XU, Cunshuan
author_sort LI, Deming
collection PubMed
description Inflammation has a dual effect: it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of IL-1R1 in liver regeneration (LR) after partial hepatectomy (PH) in aged mice. The wild-type (WT, n = 36) and the IL-1R1 knockout (KO, n = 36) 24-month-old C57BL/6J mice underwent two-thirds PH; 33 WT mice underwent sham operation. Liver coefficient was calculated by liver/body weight. The mRNA and protein expressions of genes were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting methods, respectively. Compared with WT mice, liver coefficient was lower in the IL-1R1 KO aged mice at 168 and 192 h (p = 0.039 and p = 0.027). The mRNA transcription of inflammation-related genes and cell cycle-associated genes decreased or delayed. The protein expressions of proliferation-related marker PCNA and proliferation-associated signaling pathway components JNK1, NF-κB and STAT3 reduced or retarded. There was stronger activation of proapoptotic proteins caspase-3, caspase-8 and BAX in the IL-1R1 KO mice at different time points (p < 0.05 or p < 0.01). IL-1R1 KO reduced inflammation and caused impaired liver regeneration after 2/3 partial hepatectomy in aged mice. Maintaining proper inflammation may contribute to regeneration after liver partly surgical resection in the elderly.
format Online
Article
Text
id pubmed-8068764
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Scientific and Technological Research Council of Turkey
record_format MEDLINE/PubMed
spelling pubmed-80687642021-04-26 IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice LI, Deming WANG, Ze ZHANG, Chunyan XU, Cunshuan Turk J Biol Article Inflammation has a dual effect: it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of IL-1R1 in liver regeneration (LR) after partial hepatectomy (PH) in aged mice. The wild-type (WT, n = 36) and the IL-1R1 knockout (KO, n = 36) 24-month-old C57BL/6J mice underwent two-thirds PH; 33 WT mice underwent sham operation. Liver coefficient was calculated by liver/body weight. The mRNA and protein expressions of genes were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting methods, respectively. Compared with WT mice, liver coefficient was lower in the IL-1R1 KO aged mice at 168 and 192 h (p = 0.039 and p = 0.027). The mRNA transcription of inflammation-related genes and cell cycle-associated genes decreased or delayed. The protein expressions of proliferation-related marker PCNA and proliferation-associated signaling pathway components JNK1, NF-κB and STAT3 reduced or retarded. There was stronger activation of proapoptotic proteins caspase-3, caspase-8 and BAX in the IL-1R1 KO mice at different time points (p < 0.05 or p < 0.01). IL-1R1 KO reduced inflammation and caused impaired liver regeneration after 2/3 partial hepatectomy in aged mice. Maintaining proper inflammation may contribute to regeneration after liver partly surgical resection in the elderly. The Scientific and Technological Research Council of Turkey 2021-04-20 /pmc/articles/PMC8068764/ /pubmed/33907503 http://dx.doi.org/10.3906/biy-2010-51 Text en Copyright © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Article
LI, Deming
WANG, Ze
ZHANG, Chunyan
XU, Cunshuan
IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice
title IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice
title_full IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice
title_fullStr IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice
title_full_unstemmed IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice
title_short IL-1R1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice
title_sort il-1r1 deficiency impairs liver regeneration after 2/3 partial hepatectomy in aged mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068764/
https://www.ncbi.nlm.nih.gov/pubmed/33907503
http://dx.doi.org/10.3906/biy-2010-51
work_keys_str_mv AT lideming il1r1deficiencyimpairsliverregenerationafter23partialhepatectomyinagedmice
AT wangze il1r1deficiencyimpairsliverregenerationafter23partialhepatectomyinagedmice
AT zhangchunyan il1r1deficiencyimpairsliverregenerationafter23partialhepatectomyinagedmice
AT xucunshuan il1r1deficiencyimpairsliverregenerationafter23partialhepatectomyinagedmice