Cargando…

Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2

Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism...

Descripción completa

Detalles Bibliográficos
Autores principales: CHANG, Yan, LU, Xiaofeng, QIU, Jiaying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific and Technological Research Council of Turkey 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068773/
https://www.ncbi.nlm.nih.gov/pubmed/33907500
http://dx.doi.org/10.3906/biy-2010-29
_version_ 1783683081214885888
author CHANG, Yan
LU, Xiaofeng
QIU, Jiaying
author_facet CHANG, Yan
LU, Xiaofeng
QIU, Jiaying
author_sort CHANG, Yan
collection PubMed
description Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains. Transfection of HEK293 cells with small interfering RNA and overexpression vectors of HNRNPA1 and HNRNPA2 demonstrated that HNRNPA1 and HNRNPA2 paralogs regulate each other’s expression in a compensatory manner at both the RNA and protein levels. Multiprimer reverse transcription-polymerase chain reaction showed that HNRNPA1 and HNRNPA2 did not affect splicing of the HNRNPA2 and HNRNPA1 gene. Using luciferase reporting system, we found that compensatory degradation was mediated by the 3′UTR of the two genes rather than by the promoter. Moreover, treatment with cycloheximide inhibited the compensatory regulation. Our results indicate a novel regulation mechanism of HNRNPA1 and A2 expression. Through compensatory regulation, the expression levels of HNRNPA1 and HNRNPA2 are strictly controlled within a certain range to maintain normal cellular activities under different physiological conditions.
format Online
Article
Text
id pubmed-8068773
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Scientific and Technological Research Council of Turkey
record_format MEDLINE/PubMed
spelling pubmed-80687732021-04-26 Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2 CHANG, Yan LU, Xiaofeng QIU, Jiaying Turk J Biol Article Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains. Transfection of HEK293 cells with small interfering RNA and overexpression vectors of HNRNPA1 and HNRNPA2 demonstrated that HNRNPA1 and HNRNPA2 paralogs regulate each other’s expression in a compensatory manner at both the RNA and protein levels. Multiprimer reverse transcription-polymerase chain reaction showed that HNRNPA1 and HNRNPA2 did not affect splicing of the HNRNPA2 and HNRNPA1 gene. Using luciferase reporting system, we found that compensatory degradation was mediated by the 3′UTR of the two genes rather than by the promoter. Moreover, treatment with cycloheximide inhibited the compensatory regulation. Our results indicate a novel regulation mechanism of HNRNPA1 and A2 expression. Through compensatory regulation, the expression levels of HNRNPA1 and HNRNPA2 are strictly controlled within a certain range to maintain normal cellular activities under different physiological conditions. The Scientific and Technological Research Council of Turkey 2021-04-20 /pmc/articles/PMC8068773/ /pubmed/33907500 http://dx.doi.org/10.3906/biy-2010-29 Text en Copyright © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Article
CHANG, Yan
LU, Xiaofeng
QIU, Jiaying
Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2
title Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2
title_full Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2
title_fullStr Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2
title_full_unstemmed Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2
title_short Compensatory expression regulation of highly homologous proteins HNRNPA1 and HNRNPA2
title_sort compensatory expression regulation of highly homologous proteins hnrnpa1 and hnrnpa2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068773/
https://www.ncbi.nlm.nih.gov/pubmed/33907500
http://dx.doi.org/10.3906/biy-2010-29
work_keys_str_mv AT changyan compensatoryexpressionregulationofhighlyhomologousproteinshnrnpa1andhnrnpa2
AT luxiaofeng compensatoryexpressionregulationofhighlyhomologousproteinshnrnpa1andhnrnpa2
AT qiujiaying compensatoryexpressionregulationofhighlyhomologousproteinshnrnpa1andhnrnpa2