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Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity

AST-120 (Kremezin) is used to treat progressive chronic kidney disease by adsorbing uremic toxin precursors produced by the gut microbiota, such as indole and phenols. Previously, we found that AST-120 decreased drug tolerance and virulence in Escherichia coli by adsorbing indole. Here, we show that...

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Autores principales: Hirakawa, Hidetada, Takita, Ayako, Uchida, Motoyuki, Kaneko, Yuka, Kakishima, Yuto, Tanimoto, Koichi, Kamitani, Wataru, Tomita, Haruyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068879/
https://www.ncbi.nlm.nih.gov/pubmed/33924459
http://dx.doi.org/10.3390/antibiotics10040434
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author Hirakawa, Hidetada
Takita, Ayako
Uchida, Motoyuki
Kaneko, Yuka
Kakishima, Yuto
Tanimoto, Koichi
Kamitani, Wataru
Tomita, Haruyoshi
author_facet Hirakawa, Hidetada
Takita, Ayako
Uchida, Motoyuki
Kaneko, Yuka
Kakishima, Yuto
Tanimoto, Koichi
Kamitani, Wataru
Tomita, Haruyoshi
author_sort Hirakawa, Hidetada
collection PubMed
description AST-120 (Kremezin) is used to treat progressive chronic kidney disease by adsorbing uremic toxin precursors produced by the gut microbiota, such as indole and phenols. Previously, we found that AST-120 decreased drug tolerance and virulence in Escherichia coli by adsorbing indole. Here, we show that AST-120 adsorbs phenazine compounds, such as pyocyanin, produced by Pseudomonas aeruginosa including multidrug-resistant P. aeruginosa strains, and suppresses pyocyanin-associated toxicity in A-549 (alveolar adenocarcinoma) and Caco-2 (colon adenocarcinoma) cells. Addition of fosfomycin, colistin and amikacin, which are often used to treat P. aeruginosa, inhibited the bacterial growth, regardless of the presence or absence of AST-120. These results suggest a further benefit of AST-120 that supports anti-Pseudomonas chemotherapy in addition to that of E. coli and propose a novel method to treat P. aeruginosa infection.
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spelling pubmed-80688792021-04-26 Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity Hirakawa, Hidetada Takita, Ayako Uchida, Motoyuki Kaneko, Yuka Kakishima, Yuto Tanimoto, Koichi Kamitani, Wataru Tomita, Haruyoshi Antibiotics (Basel) Article AST-120 (Kremezin) is used to treat progressive chronic kidney disease by adsorbing uremic toxin precursors produced by the gut microbiota, such as indole and phenols. Previously, we found that AST-120 decreased drug tolerance and virulence in Escherichia coli by adsorbing indole. Here, we show that AST-120 adsorbs phenazine compounds, such as pyocyanin, produced by Pseudomonas aeruginosa including multidrug-resistant P. aeruginosa strains, and suppresses pyocyanin-associated toxicity in A-549 (alveolar adenocarcinoma) and Caco-2 (colon adenocarcinoma) cells. Addition of fosfomycin, colistin and amikacin, which are often used to treat P. aeruginosa, inhibited the bacterial growth, regardless of the presence or absence of AST-120. These results suggest a further benefit of AST-120 that supports anti-Pseudomonas chemotherapy in addition to that of E. coli and propose a novel method to treat P. aeruginosa infection. MDPI 2021-04-13 /pmc/articles/PMC8068879/ /pubmed/33924459 http://dx.doi.org/10.3390/antibiotics10040434 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hirakawa, Hidetada
Takita, Ayako
Uchida, Motoyuki
Kaneko, Yuka
Kakishima, Yuto
Tanimoto, Koichi
Kamitani, Wataru
Tomita, Haruyoshi
Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity
title Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity
title_full Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity
title_fullStr Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity
title_full_unstemmed Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity
title_short Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity
title_sort adsorption of phenazines produced by pseudomonas aeruginosa using ast-120 decreases pyocyanin-associated cytotoxicity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068879/
https://www.ncbi.nlm.nih.gov/pubmed/33924459
http://dx.doi.org/10.3390/antibiotics10040434
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