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Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine

Natural killer (NK) cells were originally described as cytolytic effector cells, but since then have been recognized to possess regulatory functions on immune responses. Chemokines locate NK cells throughout the body in homeostatic and pathological conditions. They may also directly stimulate immune...

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Autores principales: Amniai, Latiffa, Ple, Coline, Barrier, Mathieu, de Nadai, Patricia, Marquillies, Philippe, Vorng, Han, Chenivesse, Cécile, Tsicopoulos, Anne, Duez, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068884/
https://www.ncbi.nlm.nih.gov/pubmed/33918621
http://dx.doi.org/10.3390/ijms22083879
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author Amniai, Latiffa
Ple, Coline
Barrier, Mathieu
de Nadai, Patricia
Marquillies, Philippe
Vorng, Han
Chenivesse, Cécile
Tsicopoulos, Anne
Duez, Catherine
author_facet Amniai, Latiffa
Ple, Coline
Barrier, Mathieu
de Nadai, Patricia
Marquillies, Philippe
Vorng, Han
Chenivesse, Cécile
Tsicopoulos, Anne
Duez, Catherine
author_sort Amniai, Latiffa
collection PubMed
description Natural killer (NK) cells were originally described as cytolytic effector cells, but since then have been recognized to possess regulatory functions on immune responses. Chemokines locate NK cells throughout the body in homeostatic and pathological conditions. They may also directly stimulate immune cells. CCL18 is a constitutive and inducible chemokine involved in allergic diseases. The aim of this study was to evaluate CCL18’s effect on NK cells from allergic and nonallergic donors in terms of both chemotactic and immune effects. Results showed that CCL18 was able to induce migration of NK cells from nonallergic donors in a G-protein-dependent manner, suggesting the involvement of a classical chemokine receptor from the family of seven-transmembrane domain G-protein-coupled receptors. In contrast, NK cells from allergic patients were unresponsive. Similarly, CCL18 was able to induce NK cell cytotoxicity only in nonallergic subjects. Purified NK cells did not express CCR8, one of the receptors described to be involved in CCL18 functions. Finally, the defect in CCL18 response by NK cells from allergic patients was unrelated to a defect in CCL18 binding to NK cells. Overall, our results suggest that some NK cell functions may be defective in allergic diseases.
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spelling pubmed-80688842021-04-26 Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine Amniai, Latiffa Ple, Coline Barrier, Mathieu de Nadai, Patricia Marquillies, Philippe Vorng, Han Chenivesse, Cécile Tsicopoulos, Anne Duez, Catherine Int J Mol Sci Article Natural killer (NK) cells were originally described as cytolytic effector cells, but since then have been recognized to possess regulatory functions on immune responses. Chemokines locate NK cells throughout the body in homeostatic and pathological conditions. They may also directly stimulate immune cells. CCL18 is a constitutive and inducible chemokine involved in allergic diseases. The aim of this study was to evaluate CCL18’s effect on NK cells from allergic and nonallergic donors in terms of both chemotactic and immune effects. Results showed that CCL18 was able to induce migration of NK cells from nonallergic donors in a G-protein-dependent manner, suggesting the involvement of a classical chemokine receptor from the family of seven-transmembrane domain G-protein-coupled receptors. In contrast, NK cells from allergic patients were unresponsive. Similarly, CCL18 was able to induce NK cell cytotoxicity only in nonallergic subjects. Purified NK cells did not express CCR8, one of the receptors described to be involved in CCL18 functions. Finally, the defect in CCL18 response by NK cells from allergic patients was unrelated to a defect in CCL18 binding to NK cells. Overall, our results suggest that some NK cell functions may be defective in allergic diseases. MDPI 2021-04-09 /pmc/articles/PMC8068884/ /pubmed/33918621 http://dx.doi.org/10.3390/ijms22083879 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amniai, Latiffa
Ple, Coline
Barrier, Mathieu
de Nadai, Patricia
Marquillies, Philippe
Vorng, Han
Chenivesse, Cécile
Tsicopoulos, Anne
Duez, Catherine
Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
title Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
title_full Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
title_fullStr Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
title_full_unstemmed Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
title_short Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
title_sort natural killer cells from allergic donors are defective in their response to ccl18 chemokine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068884/
https://www.ncbi.nlm.nih.gov/pubmed/33918621
http://dx.doi.org/10.3390/ijms22083879
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