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Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins

Background: Due to the long-term contact with metallic elements of orthodontic appliances, the potential influence of released metal ions on living organisms and the type of induced changes was investigated. Materials and Methods: Twenty-four young domestic pigs classified in two groups (experimenta...

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Autores principales: Kochanowska, Iwona Ewa, Chojnacka, Katarzyna, Pawlak-Adamska, Edyta, Mikulewicz, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068897/
https://www.ncbi.nlm.nih.gov/pubmed/33921350
http://dx.doi.org/10.3390/ma14081922
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author Kochanowska, Iwona Ewa
Chojnacka, Katarzyna
Pawlak-Adamska, Edyta
Mikulewicz, Marcin
author_facet Kochanowska, Iwona Ewa
Chojnacka, Katarzyna
Pawlak-Adamska, Edyta
Mikulewicz, Marcin
author_sort Kochanowska, Iwona Ewa
collection PubMed
description Background: Due to the long-term contact with metallic elements of orthodontic appliances, the potential influence of released metal ions on living organisms and the type of induced changes was investigated. Materials and Methods: Twenty-four young domestic pigs classified in two groups (experimental and control) were chosen as the object of this study. In the experimental group of animals, two metal plates consisting of orthodontic bands representing the mass of orthodontic appliance were mounted on the internal side of the cheek for six months. The liver, lung, and brain samples were taken post mortem from animals of both groups. The gene expression of two isoforms of metallothionein (MT-1 and MT-2) were investigated using the qPCR technique. Protein expression was confirmed by the Western blot and ELISA techniques. Results: The differences in metallothionein concentrations were observed in the lung and brain in the group of experimental animals, but not in the liver. The expression of MT-1 and MT-2 genes in the experimental vs. control group (respectively) was as follows: lung MT-1 1.04 vs. 1.11, MT-2 0.96 vs. 1.05, liver MT-1 0.89 vs. 0.91 vs. 1.12, MT-2 0.91 vs. 1.05, brain MT-1 1.24 vs. 1.20, and MT-2 0.955 vs. 0.945. These results were confirmed by gene activity, which was tested by qPCR. This increased the activity of metallothionein genes in the lungs and brain as a consequence of the release of metal ions into these tissues. The possible effects of detected change in metallothionein-2 gene expression could be the alteration of physiological functions of lung tissue. Conclusions: The effect of long-term exposure to metal orthodontic appliances on metallothioneins gene expression, as well as the induction of protein synthesis was proved.
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spelling pubmed-80688972021-04-26 Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins Kochanowska, Iwona Ewa Chojnacka, Katarzyna Pawlak-Adamska, Edyta Mikulewicz, Marcin Materials (Basel) Article Background: Due to the long-term contact with metallic elements of orthodontic appliances, the potential influence of released metal ions on living organisms and the type of induced changes was investigated. Materials and Methods: Twenty-four young domestic pigs classified in two groups (experimental and control) were chosen as the object of this study. In the experimental group of animals, two metal plates consisting of orthodontic bands representing the mass of orthodontic appliance were mounted on the internal side of the cheek for six months. The liver, lung, and brain samples were taken post mortem from animals of both groups. The gene expression of two isoforms of metallothionein (MT-1 and MT-2) were investigated using the qPCR technique. Protein expression was confirmed by the Western blot and ELISA techniques. Results: The differences in metallothionein concentrations were observed in the lung and brain in the group of experimental animals, but not in the liver. The expression of MT-1 and MT-2 genes in the experimental vs. control group (respectively) was as follows: lung MT-1 1.04 vs. 1.11, MT-2 0.96 vs. 1.05, liver MT-1 0.89 vs. 0.91 vs. 1.12, MT-2 0.91 vs. 1.05, brain MT-1 1.24 vs. 1.20, and MT-2 0.955 vs. 0.945. These results were confirmed by gene activity, which was tested by qPCR. This increased the activity of metallothionein genes in the lungs and brain as a consequence of the release of metal ions into these tissues. The possible effects of detected change in metallothionein-2 gene expression could be the alteration of physiological functions of lung tissue. Conclusions: The effect of long-term exposure to metal orthodontic appliances on metallothioneins gene expression, as well as the induction of protein synthesis was proved. MDPI 2021-04-12 /pmc/articles/PMC8068897/ /pubmed/33921350 http://dx.doi.org/10.3390/ma14081922 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kochanowska, Iwona Ewa
Chojnacka, Katarzyna
Pawlak-Adamska, Edyta
Mikulewicz, Marcin
Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins
title Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins
title_full Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins
title_fullStr Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins
title_full_unstemmed Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins
title_short Metallic Orthodontic Materials Induce Gene Expression and Protein Synthesis of Metallothioneins
title_sort metallic orthodontic materials induce gene expression and protein synthesis of metallothioneins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068897/
https://www.ncbi.nlm.nih.gov/pubmed/33921350
http://dx.doi.org/10.3390/ma14081922
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