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Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces

Background: The incidence of poliovirus has been significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged. Previously, our team reported in the Lésio-Louna-Léfini Nature Reserve (Republic of Congo) the presence of a new Enter...

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Autores principales: Sereme, Youssouf, Zarza, Sandra Madariaga, Medkour, Hacène, Amona, Inestin, Fenollar, Florence, Akiana, Jean, Mezouar, Soraya, Orain, Nicolas, Vitte, Joana, Davoust, Bernard, Raoult, Didier, Mediannikov, Oleg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068960/
https://www.ncbi.nlm.nih.gov/pubmed/33921300
http://dx.doi.org/10.3390/microorganisms9040810
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author Sereme, Youssouf
Zarza, Sandra Madariaga
Medkour, Hacène
Amona, Inestin
Fenollar, Florence
Akiana, Jean
Mezouar, Soraya
Orain, Nicolas
Vitte, Joana
Davoust, Bernard
Raoult, Didier
Mediannikov, Oleg
author_facet Sereme, Youssouf
Zarza, Sandra Madariaga
Medkour, Hacène
Amona, Inestin
Fenollar, Florence
Akiana, Jean
Mezouar, Soraya
Orain, Nicolas
Vitte, Joana
Davoust, Bernard
Raoult, Didier
Mediannikov, Oleg
author_sort Sereme, Youssouf
collection PubMed
description Background: The incidence of poliovirus has been significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged. Previously, our team reported in the Lésio-Louna-Léfini Nature Reserve (Republic of Congo) the presence of a new Enterovirus C (Ibou002) in a male gorilla that was put away because of clinical symptoms of facial paralysis. This new virus, isolated was from the stool samples of this gorilla but also from the excrement of an eco-guardian, is very similar to Coxsackievirus (EV-C99) as well as poliovirus 1 and 2. We hypothesised that these symptoms might be due to poliovirus infection. To test our hypothesis, we developed and optimised a non-invasive immunoassay for the detection of Enterovirus-specific antibodies in gorilla faeces that could be useful for routine serosurveillance in such cases. Methods: In order to assess the potential role of poliovirus infection, we have developed and optimised a protocol, based on the lyophilisation and solubilisation of small volumes of stool extracts from 16 gorilla and 3 humans, to detect specific antibodies by western blot and ELISA. Results: First, total immunoglobulins were detected in the concentrated stool extracts. Specific antibodies were then detected in 4/16 gorilla samples and 2/3 human samples by western blot using both the polio vaccine antigen and the Ibou002 antigen and by ELISA using the polio vaccine antigen. Humoral responses were greater with the Ibou002 antigen. Conclusion: We therefore suggest that this recombinant virus could lead to a polio-like disease in the endangered western lowland gorilla. The development of a non-invasive approach to detect microorganism-specific immunoglobulins from faecal samples opens numerous prospects for application in zoonotic infectious diseases and could revolutionise the screening of animals for important emerging infections, such as Ebola fever, rabies and coronavirus infections.
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spelling pubmed-80689602021-04-26 Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces Sereme, Youssouf Zarza, Sandra Madariaga Medkour, Hacène Amona, Inestin Fenollar, Florence Akiana, Jean Mezouar, Soraya Orain, Nicolas Vitte, Joana Davoust, Bernard Raoult, Didier Mediannikov, Oleg Microorganisms Article Background: The incidence of poliovirus has been significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged. Previously, our team reported in the Lésio-Louna-Léfini Nature Reserve (Republic of Congo) the presence of a new Enterovirus C (Ibou002) in a male gorilla that was put away because of clinical symptoms of facial paralysis. This new virus, isolated was from the stool samples of this gorilla but also from the excrement of an eco-guardian, is very similar to Coxsackievirus (EV-C99) as well as poliovirus 1 and 2. We hypothesised that these symptoms might be due to poliovirus infection. To test our hypothesis, we developed and optimised a non-invasive immunoassay for the detection of Enterovirus-specific antibodies in gorilla faeces that could be useful for routine serosurveillance in such cases. Methods: In order to assess the potential role of poliovirus infection, we have developed and optimised a protocol, based on the lyophilisation and solubilisation of small volumes of stool extracts from 16 gorilla and 3 humans, to detect specific antibodies by western blot and ELISA. Results: First, total immunoglobulins were detected in the concentrated stool extracts. Specific antibodies were then detected in 4/16 gorilla samples and 2/3 human samples by western blot using both the polio vaccine antigen and the Ibou002 antigen and by ELISA using the polio vaccine antigen. Humoral responses were greater with the Ibou002 antigen. Conclusion: We therefore suggest that this recombinant virus could lead to a polio-like disease in the endangered western lowland gorilla. The development of a non-invasive approach to detect microorganism-specific immunoglobulins from faecal samples opens numerous prospects for application in zoonotic infectious diseases and could revolutionise the screening of animals for important emerging infections, such as Ebola fever, rabies and coronavirus infections. MDPI 2021-04-12 /pmc/articles/PMC8068960/ /pubmed/33921300 http://dx.doi.org/10.3390/microorganisms9040810 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sereme, Youssouf
Zarza, Sandra Madariaga
Medkour, Hacène
Amona, Inestin
Fenollar, Florence
Akiana, Jean
Mezouar, Soraya
Orain, Nicolas
Vitte, Joana
Davoust, Bernard
Raoult, Didier
Mediannikov, Oleg
Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces
title Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces
title_full Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces
title_fullStr Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces
title_full_unstemmed Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces
title_short Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces
title_sort stool serology: development of a non-invasive immunological method for the detection of enterovirus-specific antibodies in congo gorilla faeces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068960/
https://www.ncbi.nlm.nih.gov/pubmed/33921300
http://dx.doi.org/10.3390/microorganisms9040810
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