Cargando…
Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study
Obesity and its related complications are a world-wide health problem. Dietary tocotrienols (TT) have been shown to improve obesity-associated metabolic disorders, such as hypercholesterolemia, hyperglycemia, and gut dysbiosis. This study examined the hypothesis that the antioxidant capacity of TT a...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069008/ https://www.ncbi.nlm.nih.gov/pubmed/33924335 http://dx.doi.org/10.3390/nu13041267 |
_version_ | 1783683136479035392 |
---|---|
author | Shen, Chwan-Li Ramamoorthy, Sivapriya Kaur, Gurvinder Dufour, Jannette M. Wang, Rui Mo, Huanbiao Watkins, Bruce A. |
author_facet | Shen, Chwan-Li Ramamoorthy, Sivapriya Kaur, Gurvinder Dufour, Jannette M. Wang, Rui Mo, Huanbiao Watkins, Bruce A. |
author_sort | Shen, Chwan-Li |
collection | PubMed |
description | Obesity and its related complications are a world-wide health problem. Dietary tocotrienols (TT) have been shown to improve obesity-associated metabolic disorders, such as hypercholesterolemia, hyperglycemia, and gut dysbiosis. This study examined the hypothesis that the antioxidant capacity of TT alters metabolites of oxidative stress and improves systemic metabolism. C57BL/6J mice were fed either a high-fat diet (HFD control) or HFD supplemented with 800 mg annatto-extracted TT/kg (HFD+TT800) for 14 weeks. Sera from obese mice were examined by non-targeted metabolite analysis using UHPLC/MS. Compared to the HFD group, the HFD+TT800 group had higher levels of serum metabolites, essential amino acids (lysine and methionine), sphingomyelins, phosphatidylcholine, lysophospholipids, and vitamins (pantothenate, pyridoxamine, pyridoxal, and retinol). TT-treated mice had lowered levels of serum metabolites, dicarboxylic fatty acids, and inflammatory/oxidative stress markers (trimethylamine N-oxide, kynurenate, 12,13-DiHOME, and 13-HODE + 9-HODE) compared to the control. The results suggest that TT supplementation lowered inflammation and oxidative stress (oxidized glutathione and GSH/GSSH) and improved macronutrient metabolism (carbohydrates) in obese mice. Thus, TT actions on metabolites were beneficial in reducing obesity-associated hypercholesterolemia/hyperglycemia. The effects of a non-toxic dose of TT in mice support the potential for clinical applications in obesity and metabolic disease. |
format | Online Article Text |
id | pubmed-8069008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80690082021-04-26 Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study Shen, Chwan-Li Ramamoorthy, Sivapriya Kaur, Gurvinder Dufour, Jannette M. Wang, Rui Mo, Huanbiao Watkins, Bruce A. Nutrients Article Obesity and its related complications are a world-wide health problem. Dietary tocotrienols (TT) have been shown to improve obesity-associated metabolic disorders, such as hypercholesterolemia, hyperglycemia, and gut dysbiosis. This study examined the hypothesis that the antioxidant capacity of TT alters metabolites of oxidative stress and improves systemic metabolism. C57BL/6J mice were fed either a high-fat diet (HFD control) or HFD supplemented with 800 mg annatto-extracted TT/kg (HFD+TT800) for 14 weeks. Sera from obese mice were examined by non-targeted metabolite analysis using UHPLC/MS. Compared to the HFD group, the HFD+TT800 group had higher levels of serum metabolites, essential amino acids (lysine and methionine), sphingomyelins, phosphatidylcholine, lysophospholipids, and vitamins (pantothenate, pyridoxamine, pyridoxal, and retinol). TT-treated mice had lowered levels of serum metabolites, dicarboxylic fatty acids, and inflammatory/oxidative stress markers (trimethylamine N-oxide, kynurenate, 12,13-DiHOME, and 13-HODE + 9-HODE) compared to the control. The results suggest that TT supplementation lowered inflammation and oxidative stress (oxidized glutathione and GSH/GSSH) and improved macronutrient metabolism (carbohydrates) in obese mice. Thus, TT actions on metabolites were beneficial in reducing obesity-associated hypercholesterolemia/hyperglycemia. The effects of a non-toxic dose of TT in mice support the potential for clinical applications in obesity and metabolic disease. MDPI 2021-04-13 /pmc/articles/PMC8069008/ /pubmed/33924335 http://dx.doi.org/10.3390/nu13041267 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shen, Chwan-Li Ramamoorthy, Sivapriya Kaur, Gurvinder Dufour, Jannette M. Wang, Rui Mo, Huanbiao Watkins, Bruce A. Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study |
title | Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study |
title_full | Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study |
title_fullStr | Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study |
title_full_unstemmed | Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study |
title_short | Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study |
title_sort | dietary annatto-extracted tocotrienol reduces inflammation and oxidative stress, and improves macronutrient metabolism in obese mice: a metabolic profiling study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069008/ https://www.ncbi.nlm.nih.gov/pubmed/33924335 http://dx.doi.org/10.3390/nu13041267 |
work_keys_str_mv | AT shenchwanli dietaryannattoextractedtocotrienolreducesinflammationandoxidativestressandimprovesmacronutrientmetabolisminobesemiceametabolicprofilingstudy AT ramamoorthysivapriya dietaryannattoextractedtocotrienolreducesinflammationandoxidativestressandimprovesmacronutrientmetabolisminobesemiceametabolicprofilingstudy AT kaurgurvinder dietaryannattoextractedtocotrienolreducesinflammationandoxidativestressandimprovesmacronutrientmetabolisminobesemiceametabolicprofilingstudy AT dufourjannettem dietaryannattoextractedtocotrienolreducesinflammationandoxidativestressandimprovesmacronutrientmetabolisminobesemiceametabolicprofilingstudy AT wangrui dietaryannattoextractedtocotrienolreducesinflammationandoxidativestressandimprovesmacronutrientmetabolisminobesemiceametabolicprofilingstudy AT mohuanbiao dietaryannattoextractedtocotrienolreducesinflammationandoxidativestressandimprovesmacronutrientmetabolisminobesemiceametabolicprofilingstudy AT watkinsbrucea dietaryannattoextractedtocotrienolreducesinflammationandoxidativestressandimprovesmacronutrientmetabolisminobesemiceametabolicprofilingstudy |