Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adul...

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Autores principales: Hitzenbichler, Florian, Bauernfeind, Stilla, Salzberger, Bernd, Schmidt, Barbara, Wenzel, Jürgen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069237/
https://www.ncbi.nlm.nih.gov/pubmed/33920072
http://dx.doi.org/10.3390/v13040653
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author Hitzenbichler, Florian
Bauernfeind, Stilla
Salzberger, Bernd
Schmidt, Barbara
Wenzel, Jürgen J.
author_facet Hitzenbichler, Florian
Bauernfeind, Stilla
Salzberger, Bernd
Schmidt, Barbara
Wenzel, Jürgen J.
author_sort Hitzenbichler, Florian
collection PubMed
description Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute quantification. The median concentrations and diagnostic sensitivities were [Formula: see text] copies/mL, 85% (NS), [Formula: see text] , 79% (OS) and [Formula: see text] , 85% (TW). Concentration differences were significant between NS and TW (P = 0.019). Saliva (SA) was available from 21 patients (median [Formula: see text]). OS and TW can be considered for SARS-CoV-2 diagnostics, although with slightly lower concentrations.
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spelling pubmed-80692372021-04-26 Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2 Hitzenbichler, Florian Bauernfeind, Stilla Salzberger, Bernd Schmidt, Barbara Wenzel, Jürgen J. Viruses Article Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute quantification. The median concentrations and diagnostic sensitivities were [Formula: see text] copies/mL, 85% (NS), [Formula: see text] , 79% (OS) and [Formula: see text] , 85% (TW). Concentration differences were significant between NS and TW (P = 0.019). Saliva (SA) was available from 21 patients (median [Formula: see text]). OS and TW can be considered for SARS-CoV-2 diagnostics, although with slightly lower concentrations. MDPI 2021-04-10 /pmc/articles/PMC8069237/ /pubmed/33920072 http://dx.doi.org/10.3390/v13040653 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hitzenbichler, Florian
Bauernfeind, Stilla
Salzberger, Bernd
Schmidt, Barbara
Wenzel, Jürgen J.
Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2
title Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2
title_full Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2
title_fullStr Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2
title_full_unstemmed Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2
title_short Comparison of Throat Washings, Nasopharyngeal Swabs and Oropharyngeal Swabs for Detection of SARS-CoV-2
title_sort comparison of throat washings, nasopharyngeal swabs and oropharyngeal swabs for detection of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069237/
https://www.ncbi.nlm.nih.gov/pubmed/33920072
http://dx.doi.org/10.3390/v13040653
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