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Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging

In this study, a laparoscopic imaging device and a light source able to select wavelengths by bandpass filters were developed to perform multispectral imaging (MSI) using over 1000 nm near-infrared (OTN-NIR) on regions under a laparoscope. Subsequently, MSI (wavelengths: 1000–1400 nm) was performed...

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Autores principales: Takamatsu, Toshihiro, Kitagawa, Yuichi, Akimoto, Kohei, Iwanami, Ren, Endo, Yuto, Takashima, Kenji, Okubo, Kyohei, Umezawa, Masakazu, Kuwata, Takeshi, Sato, Daiki, Kadota, Tomohiro, Mitsui, Tomohiro, Ikematsu, Hiroaki, Yokota, Hideo, Soga, Kohei, Takemura, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069262/
https://www.ncbi.nlm.nih.gov/pubmed/33918935
http://dx.doi.org/10.3390/s21082649
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author Takamatsu, Toshihiro
Kitagawa, Yuichi
Akimoto, Kohei
Iwanami, Ren
Endo, Yuto
Takashima, Kenji
Okubo, Kyohei
Umezawa, Masakazu
Kuwata, Takeshi
Sato, Daiki
Kadota, Tomohiro
Mitsui, Tomohiro
Ikematsu, Hiroaki
Yokota, Hideo
Soga, Kohei
Takemura, Hiroshi
author_facet Takamatsu, Toshihiro
Kitagawa, Yuichi
Akimoto, Kohei
Iwanami, Ren
Endo, Yuto
Takashima, Kenji
Okubo, Kyohei
Umezawa, Masakazu
Kuwata, Takeshi
Sato, Daiki
Kadota, Tomohiro
Mitsui, Tomohiro
Ikematsu, Hiroaki
Yokota, Hideo
Soga, Kohei
Takemura, Hiroshi
author_sort Takamatsu, Toshihiro
collection PubMed
description In this study, a laparoscopic imaging device and a light source able to select wavelengths by bandpass filters were developed to perform multispectral imaging (MSI) using over 1000 nm near-infrared (OTN-NIR) on regions under a laparoscope. Subsequently, MSI (wavelengths: 1000–1400 nm) was performed using the built device on nine live mice before and after tumor implantation. The normal and tumor pixels captured within the mice were used as teaching data sets, and the tumor-implanted mice data were classified using a neural network applied following a leave-one-out cross-validation procedure. The system provided a specificity of 89.5%, a sensitivity of 53.5%, and an accuracy of 87.8% for subcutaneous tumor discrimination. Aggregated true-positive (TP) pixels were confirmed in all tumor-implanted mice, which indicated that the laparoscopic OTN-NIR MSI could potentially be applied in vivo for classifying target lesions such as cancer in deep tissues.
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spelling pubmed-80692622021-04-26 Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging Takamatsu, Toshihiro Kitagawa, Yuichi Akimoto, Kohei Iwanami, Ren Endo, Yuto Takashima, Kenji Okubo, Kyohei Umezawa, Masakazu Kuwata, Takeshi Sato, Daiki Kadota, Tomohiro Mitsui, Tomohiro Ikematsu, Hiroaki Yokota, Hideo Soga, Kohei Takemura, Hiroshi Sensors (Basel) Communication In this study, a laparoscopic imaging device and a light source able to select wavelengths by bandpass filters were developed to perform multispectral imaging (MSI) using over 1000 nm near-infrared (OTN-NIR) on regions under a laparoscope. Subsequently, MSI (wavelengths: 1000–1400 nm) was performed using the built device on nine live mice before and after tumor implantation. The normal and tumor pixels captured within the mice were used as teaching data sets, and the tumor-implanted mice data were classified using a neural network applied following a leave-one-out cross-validation procedure. The system provided a specificity of 89.5%, a sensitivity of 53.5%, and an accuracy of 87.8% for subcutaneous tumor discrimination. Aggregated true-positive (TP) pixels were confirmed in all tumor-implanted mice, which indicated that the laparoscopic OTN-NIR MSI could potentially be applied in vivo for classifying target lesions such as cancer in deep tissues. MDPI 2021-04-09 /pmc/articles/PMC8069262/ /pubmed/33918935 http://dx.doi.org/10.3390/s21082649 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Takamatsu, Toshihiro
Kitagawa, Yuichi
Akimoto, Kohei
Iwanami, Ren
Endo, Yuto
Takashima, Kenji
Okubo, Kyohei
Umezawa, Masakazu
Kuwata, Takeshi
Sato, Daiki
Kadota, Tomohiro
Mitsui, Tomohiro
Ikematsu, Hiroaki
Yokota, Hideo
Soga, Kohei
Takemura, Hiroshi
Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging
title Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging
title_full Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging
title_fullStr Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging
title_full_unstemmed Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging
title_short Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging
title_sort over 1000 nm near-infrared multispectral imaging system for laparoscopic in vivo imaging
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069262/
https://www.ncbi.nlm.nih.gov/pubmed/33918935
http://dx.doi.org/10.3390/s21082649
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