Cargando…

Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens

SIMPLE SUMMARY: In recent years, a considerable expansion in the use of essential oils as feed additives in animal nutrition has occurred, mainly as an alternative to antibiotic growth promoters worldwide. The objective of the present research was to evaluate dietary supplementation of essential oil...

Descripción completa

Detalles Bibliográficos
Autores principales: Coles, Makenly E., Forga, Aaron J., Señas-Cuesta, Roberto, Graham, Brittany D., Selby, Callie M., Uribe, Álvaro J., Martínez, Blanca C., Angel-Isaza, Jaime A., Vuong, Christine N., Hernandez-Velasco, Xochitl, Hargis, Billy M., Tellez-Isaias, Guillermo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069271/
https://www.ncbi.nlm.nih.gov/pubmed/33924404
http://dx.doi.org/10.3390/ani11041111
_version_ 1783683198114332672
author Coles, Makenly E.
Forga, Aaron J.
Señas-Cuesta, Roberto
Graham, Brittany D.
Selby, Callie M.
Uribe, Álvaro J.
Martínez, Blanca C.
Angel-Isaza, Jaime A.
Vuong, Christine N.
Hernandez-Velasco, Xochitl
Hargis, Billy M.
Tellez-Isaias, Guillermo
author_facet Coles, Makenly E.
Forga, Aaron J.
Señas-Cuesta, Roberto
Graham, Brittany D.
Selby, Callie M.
Uribe, Álvaro J.
Martínez, Blanca C.
Angel-Isaza, Jaime A.
Vuong, Christine N.
Hernandez-Velasco, Xochitl
Hargis, Billy M.
Tellez-Isaias, Guillermo
author_sort Coles, Makenly E.
collection PubMed
description SIMPLE SUMMARY: In recent years, a considerable expansion in the use of essential oils as feed additives in animal nutrition has occurred, mainly as an alternative to antibiotic growth promoters worldwide. The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on the in vitro proliferation of Clostridium perfringens (CP), as well as the performance, intestinal integrity, and necrotic enteritis lesions using a previously established necrotic enteritis challenge model in broiler chickens. The supernatant from digested feed supplemented with LEO and inoculated with CP reduced CP in vitro compared with the positive control. LEO showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction in NE lesion scores, morbidity and mortality compared with the positive challenge control group These results suggest that the dietary inclusion of L. origanoides could mitigate some of the complex negative impacts caused by necrotic enteritis. ABSTRACT: The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), Eimeria maxima (day 18), and C. perfringens (CP, days 22-23); Group 3: dietary supplementation LEO and challenged. On d 25 of age, serum samples were collected to evaluate fluorescein isothiocyanate-dextran (FITC-d), superoxide dismutase (SOD), gamma interferon (IFN-γ), Immunoglobulin A (IgA). Group 3 showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction in NE lesion scores, morbidity and mortality compared with the positive challenge control group (p < 0.05) compared with Group 2. Digested feed supernatant, supplemented with LEO and inoculated with CP, reduced CP burden (p < 0.05). Group 3 also exhibited a significant reduction in FITC-d, IFN-γ and IgA compared with Group 2. However, a significant increase SOD was observed in Group 3 compared with both control groups. Further investigation to compare the effect of LEO and the standard treatment of clostridial NE is required.
format Online
Article
Text
id pubmed-8069271
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80692712021-04-26 Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens Coles, Makenly E. Forga, Aaron J. Señas-Cuesta, Roberto Graham, Brittany D. Selby, Callie M. Uribe, Álvaro J. Martínez, Blanca C. Angel-Isaza, Jaime A. Vuong, Christine N. Hernandez-Velasco, Xochitl Hargis, Billy M. Tellez-Isaias, Guillermo Animals (Basel) Article SIMPLE SUMMARY: In recent years, a considerable expansion in the use of essential oils as feed additives in animal nutrition has occurred, mainly as an alternative to antibiotic growth promoters worldwide. The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on the in vitro proliferation of Clostridium perfringens (CP), as well as the performance, intestinal integrity, and necrotic enteritis lesions using a previously established necrotic enteritis challenge model in broiler chickens. The supernatant from digested feed supplemented with LEO and inoculated with CP reduced CP in vitro compared with the positive control. LEO showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction in NE lesion scores, morbidity and mortality compared with the positive challenge control group These results suggest that the dietary inclusion of L. origanoides could mitigate some of the complex negative impacts caused by necrotic enteritis. ABSTRACT: The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), Eimeria maxima (day 18), and C. perfringens (CP, days 22-23); Group 3: dietary supplementation LEO and challenged. On d 25 of age, serum samples were collected to evaluate fluorescein isothiocyanate-dextran (FITC-d), superoxide dismutase (SOD), gamma interferon (IFN-γ), Immunoglobulin A (IgA). Group 3 showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction in NE lesion scores, morbidity and mortality compared with the positive challenge control group (p < 0.05) compared with Group 2. Digested feed supernatant, supplemented with LEO and inoculated with CP, reduced CP burden (p < 0.05). Group 3 also exhibited a significant reduction in FITC-d, IFN-γ and IgA compared with Group 2. However, a significant increase SOD was observed in Group 3 compared with both control groups. Further investigation to compare the effect of LEO and the standard treatment of clostridial NE is required. MDPI 2021-04-13 /pmc/articles/PMC8069271/ /pubmed/33924404 http://dx.doi.org/10.3390/ani11041111 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Coles, Makenly E.
Forga, Aaron J.
Señas-Cuesta, Roberto
Graham, Brittany D.
Selby, Callie M.
Uribe, Álvaro J.
Martínez, Blanca C.
Angel-Isaza, Jaime A.
Vuong, Christine N.
Hernandez-Velasco, Xochitl
Hargis, Billy M.
Tellez-Isaias, Guillermo
Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens
title Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens
title_full Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens
title_fullStr Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens
title_full_unstemmed Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens
title_short Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens
title_sort assessment of lippia origanoides essential oils in a salmonella typhimurium, eimeria maxima, and clostridium perfringens challenge model to induce necrotic enteritis in broiler chickens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069271/
https://www.ncbi.nlm.nih.gov/pubmed/33924404
http://dx.doi.org/10.3390/ani11041111
work_keys_str_mv AT colesmakenlye assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT forgaaaronj assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT senascuestaroberto assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT grahambrittanyd assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT selbycalliem assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT uribealvaroj assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT martinezblancac assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT angelisazajaimea assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT vuongchristinen assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT hernandezvelascoxochitl assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT hargisbillym assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens
AT tellezisaiasguillermo assessmentoflippiaoriganoidesessentialoilsinasalmonellatyphimuriumeimeriamaximaandclostridiumperfringenschallengemodeltoinducenecroticenteritisinbroilerchickens