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Screening and Identification of Host Proteins Interacting with Iris lactea var. chinensis Metallothionein IlMT2a by Yeast Two-Hybrid Assay

Iris lactea var. chinensis (Fisch.) (I. lactea var. chinensis) is a well-known cadmium (Cd)-tolerant plant and we have previously shown that the metallothionein gene, IlMT2a, of the plant may be playing a key role in conferring the Cd tolerance. In this study, we have identified several proteins int...

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Autores principales: Wang, Zhiquan, Ni, Longjie, Liu, Liangqin, Yuan, Haiyan, Huang, Suzhen, Gu, Chunsun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069374/
https://www.ncbi.nlm.nih.gov/pubmed/33920321
http://dx.doi.org/10.3390/genes12040554
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author Wang, Zhiquan
Ni, Longjie
Liu, Liangqin
Yuan, Haiyan
Huang, Suzhen
Gu, Chunsun
author_facet Wang, Zhiquan
Ni, Longjie
Liu, Liangqin
Yuan, Haiyan
Huang, Suzhen
Gu, Chunsun
author_sort Wang, Zhiquan
collection PubMed
description Iris lactea var. chinensis (Fisch.) (I. lactea var. chinensis) is a well-known cadmium (Cd)-tolerant plant and we have previously shown that the metallothionein gene, IlMT2a, of the plant may be playing a key role in conferring the Cd tolerance. In this study, we have identified several proteins interacting with the IlMT2a by screening yeast two-hybrid library constructed from cDNAs isolated from Cd-treated I. lacteal var. chinensis plants. Putative functions of these proteins include those involved in photosynthesis, ROS scavenge, nutrient transport, and transcriptional regulation, to name a few. In particular, another metallothionein, which we assigned the name of IlMT3, was identified as an interacting partner of the IlMT2a. Unlike IlMT2a, it did not provide any significant protection against Cd toxicity in transgenic Arabidopsis thaliana L. (A. thaliana). To our knowledge, this is the first time ever reporting the interaction of two metallothionein proteins in plants. Learning the biological significance of the interaction between IlMT2a and IlMT3 would be the focus of future study and would be able to provide valuable insights into the understanding plant metallothionein’s diverse and complex roles in coordinating many important cellular physiologies including stress responses, gene regulations, and energy metabolisms.
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spelling pubmed-80693742021-04-26 Screening and Identification of Host Proteins Interacting with Iris lactea var. chinensis Metallothionein IlMT2a by Yeast Two-Hybrid Assay Wang, Zhiquan Ni, Longjie Liu, Liangqin Yuan, Haiyan Huang, Suzhen Gu, Chunsun Genes (Basel) Article Iris lactea var. chinensis (Fisch.) (I. lactea var. chinensis) is a well-known cadmium (Cd)-tolerant plant and we have previously shown that the metallothionein gene, IlMT2a, of the plant may be playing a key role in conferring the Cd tolerance. In this study, we have identified several proteins interacting with the IlMT2a by screening yeast two-hybrid library constructed from cDNAs isolated from Cd-treated I. lacteal var. chinensis plants. Putative functions of these proteins include those involved in photosynthesis, ROS scavenge, nutrient transport, and transcriptional regulation, to name a few. In particular, another metallothionein, which we assigned the name of IlMT3, was identified as an interacting partner of the IlMT2a. Unlike IlMT2a, it did not provide any significant protection against Cd toxicity in transgenic Arabidopsis thaliana L. (A. thaliana). To our knowledge, this is the first time ever reporting the interaction of two metallothionein proteins in plants. Learning the biological significance of the interaction between IlMT2a and IlMT3 would be the focus of future study and would be able to provide valuable insights into the understanding plant metallothionein’s diverse and complex roles in coordinating many important cellular physiologies including stress responses, gene regulations, and energy metabolisms. MDPI 2021-04-10 /pmc/articles/PMC8069374/ /pubmed/33920321 http://dx.doi.org/10.3390/genes12040554 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Zhiquan
Ni, Longjie
Liu, Liangqin
Yuan, Haiyan
Huang, Suzhen
Gu, Chunsun
Screening and Identification of Host Proteins Interacting with Iris lactea var. chinensis Metallothionein IlMT2a by Yeast Two-Hybrid Assay
title Screening and Identification of Host Proteins Interacting with Iris lactea var. chinensis Metallothionein IlMT2a by Yeast Two-Hybrid Assay
title_full Screening and Identification of Host Proteins Interacting with Iris lactea var. chinensis Metallothionein IlMT2a by Yeast Two-Hybrid Assay
title_fullStr Screening and Identification of Host Proteins Interacting with Iris lactea var. chinensis Metallothionein IlMT2a by Yeast Two-Hybrid Assay
title_full_unstemmed Screening and Identification of Host Proteins Interacting with Iris lactea var. chinensis Metallothionein IlMT2a by Yeast Two-Hybrid Assay
title_short Screening and Identification of Host Proteins Interacting with Iris lactea var. chinensis Metallothionein IlMT2a by Yeast Two-Hybrid Assay
title_sort screening and identification of host proteins interacting with iris lactea var. chinensis metallothionein ilmt2a by yeast two-hybrid assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069374/
https://www.ncbi.nlm.nih.gov/pubmed/33920321
http://dx.doi.org/10.3390/genes12040554
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