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Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis

Background: Multiple sclerosis (MS) is frequently characterized by a variety of clinical signs, often exhibiting little specificity. The diagnosis requires a combination of medical observations and instrumental tests, and any support for its objective assessment is helpful. Objective: Herein, we des...

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Autores principales: Annesi, Ferdinanda, Hermoso-Durán, Sonia, Rizzuti, Bruno, Bruno, Rosalinda, Pirritano, Domenico, Petrone, Alfredo, Del Giudice, Francesco, Ojeda, Jorge, Vega, Sonia, Sanchez-Gracia, Oscar, Velazquez-Campoy, Adrian, Abian, Olga, Guzzi, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069382/
https://www.ncbi.nlm.nih.gov/pubmed/33924346
http://dx.doi.org/10.3390/jpm11040295
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author Annesi, Ferdinanda
Hermoso-Durán, Sonia
Rizzuti, Bruno
Bruno, Rosalinda
Pirritano, Domenico
Petrone, Alfredo
Del Giudice, Francesco
Ojeda, Jorge
Vega, Sonia
Sanchez-Gracia, Oscar
Velazquez-Campoy, Adrian
Abian, Olga
Guzzi, Rita
author_facet Annesi, Ferdinanda
Hermoso-Durán, Sonia
Rizzuti, Bruno
Bruno, Rosalinda
Pirritano, Domenico
Petrone, Alfredo
Del Giudice, Francesco
Ojeda, Jorge
Vega, Sonia
Sanchez-Gracia, Oscar
Velazquez-Campoy, Adrian
Abian, Olga
Guzzi, Rita
author_sort Annesi, Ferdinanda
collection PubMed
description Background: Multiple sclerosis (MS) is frequently characterized by a variety of clinical signs, often exhibiting little specificity. The diagnosis requires a combination of medical observations and instrumental tests, and any support for its objective assessment is helpful. Objective: Herein, we describe the application of thermal liquid biopsy (TLB) of blood plasma samples, a methodology for predicting the occurrence of MS with a noninvasive, quick blood test. Methods: TLB allows one to define an index (TLB score), which provides information about overall real-time alterations in plasma proteome that may be indicative of MS. Results: This pilot study, based on 85 subjects (45 MS patients and 40 controls), showed good performance indexes (sensitivity and specificity both around 70%). The diagnostic methods better discriminate between early stage and low-burden MS patients, and it is not influenced by gender, age, or assumption of therapeutic drugs. TLB is more accurate for patients having low disability level (≤ 3.0, measured by the expanded disability status scale, EDSS) and a relapsing–remitting diagnosis. Conclusion: Our results suggest that TLB can be applied to MS, especially in an initial phase of the disease when diagnosis is difficult and yet more important (in such cases, accuracy of prediction is close to 80%), as well as in personalized patient periodic monitoring. The next step will be determining its utility in differentiating between MS and other disorders, in particular in inflammatory diseases.
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spelling pubmed-80693822021-04-26 Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis Annesi, Ferdinanda Hermoso-Durán, Sonia Rizzuti, Bruno Bruno, Rosalinda Pirritano, Domenico Petrone, Alfredo Del Giudice, Francesco Ojeda, Jorge Vega, Sonia Sanchez-Gracia, Oscar Velazquez-Campoy, Adrian Abian, Olga Guzzi, Rita J Pers Med Article Background: Multiple sclerosis (MS) is frequently characterized by a variety of clinical signs, often exhibiting little specificity. The diagnosis requires a combination of medical observations and instrumental tests, and any support for its objective assessment is helpful. Objective: Herein, we describe the application of thermal liquid biopsy (TLB) of blood plasma samples, a methodology for predicting the occurrence of MS with a noninvasive, quick blood test. Methods: TLB allows one to define an index (TLB score), which provides information about overall real-time alterations in plasma proteome that may be indicative of MS. Results: This pilot study, based on 85 subjects (45 MS patients and 40 controls), showed good performance indexes (sensitivity and specificity both around 70%). The diagnostic methods better discriminate between early stage and low-burden MS patients, and it is not influenced by gender, age, or assumption of therapeutic drugs. TLB is more accurate for patients having low disability level (≤ 3.0, measured by the expanded disability status scale, EDSS) and a relapsing–remitting diagnosis. Conclusion: Our results suggest that TLB can be applied to MS, especially in an initial phase of the disease when diagnosis is difficult and yet more important (in such cases, accuracy of prediction is close to 80%), as well as in personalized patient periodic monitoring. The next step will be determining its utility in differentiating between MS and other disorders, in particular in inflammatory diseases. MDPI 2021-04-13 /pmc/articles/PMC8069382/ /pubmed/33924346 http://dx.doi.org/10.3390/jpm11040295 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Annesi, Ferdinanda
Hermoso-Durán, Sonia
Rizzuti, Bruno
Bruno, Rosalinda
Pirritano, Domenico
Petrone, Alfredo
Del Giudice, Francesco
Ojeda, Jorge
Vega, Sonia
Sanchez-Gracia, Oscar
Velazquez-Campoy, Adrian
Abian, Olga
Guzzi, Rita
Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis
title Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis
title_full Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis
title_fullStr Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis
title_full_unstemmed Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis
title_short Thermal Liquid Biopsy (TLB) of Blood Plasma as a Potential Tool to Help in the Early Diagnosis of Multiple Sclerosis
title_sort thermal liquid biopsy (tlb) of blood plasma as a potential tool to help in the early diagnosis of multiple sclerosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069382/
https://www.ncbi.nlm.nih.gov/pubmed/33924346
http://dx.doi.org/10.3390/jpm11040295
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