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Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration

Quercetin is a member of the flavonoid group of compounds, which is abundantly present in various dietary sources. It has excellent antioxidant properties and anti-inflammatory activity and is very effective as an anti-cancer agent against various types of tumors, both in vivo and in vitro. Querceti...

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Autores principales: Abruzzese, Vittorio, Matera, Ilenia, Martinelli, Fabio, Carmosino, Monica, Koshal, Prashant, Milella, Luigi, Bisaccia, Faustino, Ostuni, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069417/
https://www.ncbi.nlm.nih.gov/pubmed/33918053
http://dx.doi.org/10.3390/ijms22083871
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author Abruzzese, Vittorio
Matera, Ilenia
Martinelli, Fabio
Carmosino, Monica
Koshal, Prashant
Milella, Luigi
Bisaccia, Faustino
Ostuni, Angela
author_facet Abruzzese, Vittorio
Matera, Ilenia
Martinelli, Fabio
Carmosino, Monica
Koshal, Prashant
Milella, Luigi
Bisaccia, Faustino
Ostuni, Angela
author_sort Abruzzese, Vittorio
collection PubMed
description Quercetin is a member of the flavonoid group of compounds, which is abundantly present in various dietary sources. It has excellent antioxidant properties and anti-inflammatory activity and is very effective as an anti-cancer agent against various types of tumors, both in vivo and in vitro. Quercetin has been also reported to modulate the activity of some members of the multidrug-resistance transporters family, such as P-gp, ABCC1, ABCC2, and ABCG2, and the activity of ecto-5′-nucleotidase (NT5E/CD73), a key regulator in some tumor processes such as invasion, migration, and metastasis. In this study, we investigated the effect of Quercetin on ABCC6 expression in HepG2 cells. ABCC6 is a member of the superfamily of ATP-binding cassette (ABC) transporters, poorly involved in drug resistance, whose mutations cause pseudoxanthoma elasticum, an inherited disease characterized by ectopic calcification of soft connective tissues. Recently, it has been reported that ABCC6 contributes to cytoskeleton rearrangements and HepG2 cell motility through purinergic signaling. Gene and protein expression were evaluated by quantitative Reverse-Transcription PCR (RT-qPCR) and western blot, respectively. Actin cytoskeleton dynamics was evaluated by laser confocal microscopy using fluorophore-conjugated phalloidin. Cell motility was analyzed by an in vitro wound-healing migration assay. We propose that ABCC6 expression may be controlled by the AKT pathway as part of an adaptative response to oxidative stress, which can be mitigated by the use of Quercetin-like flavonoids.
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spelling pubmed-80694172021-04-26 Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration Abruzzese, Vittorio Matera, Ilenia Martinelli, Fabio Carmosino, Monica Koshal, Prashant Milella, Luigi Bisaccia, Faustino Ostuni, Angela Int J Mol Sci Article Quercetin is a member of the flavonoid group of compounds, which is abundantly present in various dietary sources. It has excellent antioxidant properties and anti-inflammatory activity and is very effective as an anti-cancer agent against various types of tumors, both in vivo and in vitro. Quercetin has been also reported to modulate the activity of some members of the multidrug-resistance transporters family, such as P-gp, ABCC1, ABCC2, and ABCG2, and the activity of ecto-5′-nucleotidase (NT5E/CD73), a key regulator in some tumor processes such as invasion, migration, and metastasis. In this study, we investigated the effect of Quercetin on ABCC6 expression in HepG2 cells. ABCC6 is a member of the superfamily of ATP-binding cassette (ABC) transporters, poorly involved in drug resistance, whose mutations cause pseudoxanthoma elasticum, an inherited disease characterized by ectopic calcification of soft connective tissues. Recently, it has been reported that ABCC6 contributes to cytoskeleton rearrangements and HepG2 cell motility through purinergic signaling. Gene and protein expression were evaluated by quantitative Reverse-Transcription PCR (RT-qPCR) and western blot, respectively. Actin cytoskeleton dynamics was evaluated by laser confocal microscopy using fluorophore-conjugated phalloidin. Cell motility was analyzed by an in vitro wound-healing migration assay. We propose that ABCC6 expression may be controlled by the AKT pathway as part of an adaptative response to oxidative stress, which can be mitigated by the use of Quercetin-like flavonoids. MDPI 2021-04-08 /pmc/articles/PMC8069417/ /pubmed/33918053 http://dx.doi.org/10.3390/ijms22083871 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Abruzzese, Vittorio
Matera, Ilenia
Martinelli, Fabio
Carmosino, Monica
Koshal, Prashant
Milella, Luigi
Bisaccia, Faustino
Ostuni, Angela
Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration
title Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration
title_full Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration
title_fullStr Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration
title_full_unstemmed Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration
title_short Effect of Quercetin on ABCC6 Transporter: Implication in HepG2 Migration
title_sort effect of quercetin on abcc6 transporter: implication in hepg2 migration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069417/
https://www.ncbi.nlm.nih.gov/pubmed/33918053
http://dx.doi.org/10.3390/ijms22083871
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