Cargando…

Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea

Cucumber powdery mildew caused by Sphaerotheca fuliginea is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes CsRSF1 and CsRSF2, which participated in regulating the resistance of cucumber t...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xue, Chen, Qiumin, Huang, Jingnan, Meng, Xiangnan, Cui, Na, Yu, Yang, Fan, Haiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069588/
https://www.ncbi.nlm.nih.gov/pubmed/33924330
http://dx.doi.org/10.3390/ijms22083986
_version_ 1783683272430059520
author Wang, Xue
Chen, Qiumin
Huang, Jingnan
Meng, Xiangnan
Cui, Na
Yu, Yang
Fan, Haiyan
author_facet Wang, Xue
Chen, Qiumin
Huang, Jingnan
Meng, Xiangnan
Cui, Na
Yu, Yang
Fan, Haiyan
author_sort Wang, Xue
collection PubMed
description Cucumber powdery mildew caused by Sphaerotheca fuliginea is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes CsRSF1 and CsRSF2, which participated in regulating the resistance of cucumber to S. fuliginea. The subcellular localization showed that the CsRSF1 protein was localized in the nucleus, cytoplasm, and cell membrane, while the CsRSF2 protein was localized in the cell membrane and cytoplasm. In addition, the transcript levels of CsRSF1 and CsRSF2 were different between resistant and susceptible cultivars after treatment with exogenous substances, such as abscisic acid (ABA), methyl jasmonate (MeJA), salicylic acid (SA), ethephon (ETH), gibberellin (GA) and hydrogen peroxide (H(2)O(2)). The expression analysis showed that the transcript levels of CsRSF1 and CsRSF2 were correlated with plant defense response against S. fuliginea. Moreover, the silencing of CsRSF1 and CsRSF2 impaired host resistance to S. fuliginea, but CsRSF1 and CsRSF2 overexpression improved resistance to S. fuliginea in cucumber. These results showed that CsRSF1 and CsRSF2 genes positively contributed to the resistance of cucumber to S. fuliginea. At the same time, CsRSF1 and CsRSF2 genes could also regulate the expression of defense-related genes. The findings of this study might help enhance the resistance of cucumber to S. fuliginea.
format Online
Article
Text
id pubmed-8069588
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80695882021-04-26 Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea Wang, Xue Chen, Qiumin Huang, Jingnan Meng, Xiangnan Cui, Na Yu, Yang Fan, Haiyan Int J Mol Sci Article Cucumber powdery mildew caused by Sphaerotheca fuliginea is a leaf disease that seriously affects cucumber’s yield and quality. This study aimed to report two nucleotide-binding site-leucine-rich repeats (NBS-LRR) genes CsRSF1 and CsRSF2, which participated in regulating the resistance of cucumber to S. fuliginea. The subcellular localization showed that the CsRSF1 protein was localized in the nucleus, cytoplasm, and cell membrane, while the CsRSF2 protein was localized in the cell membrane and cytoplasm. In addition, the transcript levels of CsRSF1 and CsRSF2 were different between resistant and susceptible cultivars after treatment with exogenous substances, such as abscisic acid (ABA), methyl jasmonate (MeJA), salicylic acid (SA), ethephon (ETH), gibberellin (GA) and hydrogen peroxide (H(2)O(2)). The expression analysis showed that the transcript levels of CsRSF1 and CsRSF2 were correlated with plant defense response against S. fuliginea. Moreover, the silencing of CsRSF1 and CsRSF2 impaired host resistance to S. fuliginea, but CsRSF1 and CsRSF2 overexpression improved resistance to S. fuliginea in cucumber. These results showed that CsRSF1 and CsRSF2 genes positively contributed to the resistance of cucumber to S. fuliginea. At the same time, CsRSF1 and CsRSF2 genes could also regulate the expression of defense-related genes. The findings of this study might help enhance the resistance of cucumber to S. fuliginea. MDPI 2021-04-13 /pmc/articles/PMC8069588/ /pubmed/33924330 http://dx.doi.org/10.3390/ijms22083986 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xue
Chen, Qiumin
Huang, Jingnan
Meng, Xiangnan
Cui, Na
Yu, Yang
Fan, Haiyan
Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_full Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_fullStr Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_full_unstemmed Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_short Nucleotide-Binding Leucine-Rich Repeat Genes CsRSF1 and CsRSF2 Are Positive Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
title_sort nucleotide-binding leucine-rich repeat genes csrsf1 and csrsf2 are positive modulators in the cucumis sativus defense response to sphaerotheca fuliginea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069588/
https://www.ncbi.nlm.nih.gov/pubmed/33924330
http://dx.doi.org/10.3390/ijms22083986
work_keys_str_mv AT wangxue nucleotidebindingleucinerichrepeatgenescsrsf1andcsrsf2arepositivemodulatorsinthecucumissativusdefenseresponsetosphaerothecafuliginea
AT chenqiumin nucleotidebindingleucinerichrepeatgenescsrsf1andcsrsf2arepositivemodulatorsinthecucumissativusdefenseresponsetosphaerothecafuliginea
AT huangjingnan nucleotidebindingleucinerichrepeatgenescsrsf1andcsrsf2arepositivemodulatorsinthecucumissativusdefenseresponsetosphaerothecafuliginea
AT mengxiangnan nucleotidebindingleucinerichrepeatgenescsrsf1andcsrsf2arepositivemodulatorsinthecucumissativusdefenseresponsetosphaerothecafuliginea
AT cuina nucleotidebindingleucinerichrepeatgenescsrsf1andcsrsf2arepositivemodulatorsinthecucumissativusdefenseresponsetosphaerothecafuliginea
AT yuyang nucleotidebindingleucinerichrepeatgenescsrsf1andcsrsf2arepositivemodulatorsinthecucumissativusdefenseresponsetosphaerothecafuliginea
AT fanhaiyan nucleotidebindingleucinerichrepeatgenescsrsf1andcsrsf2arepositivemodulatorsinthecucumissativusdefenseresponsetosphaerothecafuliginea