A Mother’s Story, Mitogenome Relationships in the Genus Rupicapra

SIMPLE SUMMARY: Two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently recognized by taxonomy and further subdivided into seven and three subspecies, respectively. However, recent research based on molecular markers finds this classification questionable. We aim to increase the resolution of published research on chamois phylogeny by including mitogenomes of all available subspecies, including the previously unpublished mitogenomes of R. r. balcanica and R. r. tatrica subspecies. The inferred phylogeny based on the full mitogenomes confirms the previously reported genus subdivision in three clades and its monophyletic positioning within the Caprinae. Phylogeny and taxonomy of Rupicapra species thus remain controversial prompting for the inclusion of archeological remains to solve the controversy. ABSTRACT: Although the two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently classified as least-concern by the IUCN (International Union for Conservation of Nature), inconsistencies on the subspecies classification reported in literature make it challenging to assess the conservation status of the single subspecies. Previous studies relying on mitochondrial genes, sometimes in combination with nuclear or Y-chromosome markers, reported the presence of clusters corresponding to the geographic distribution but highlighting ambiguities in the genus phylogeny. Here we report novel de novo assembled sequences of the mitochondrial genome from nine individuals, including previously unpublished R. r. balcanica and R. r. tatrica subspecies, and use them to untangle the genus phylogeny. Our results based on the full mitogenome inferred phylogeny confirm the previously reported genus subdivision in three clades and its monophyletic positioning within the Caprinae. Phylogeny and taxonomy of Rupicapra species thus remain controversial prompting for the inclusion of archeological remains to solve the controversy.