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The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia
Loss of sialic acid from the carbohydrate side chains of platelet glycoproteins can affect platelet clearance, a proposed mechanism involved in the etiopathogenesis of immune thrombocytopaenia (ITP). We aimed to assess whether changes in platelet glycosylation in patients with ITP affected platelet...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069668/ https://www.ncbi.nlm.nih.gov/pubmed/33924503 http://dx.doi.org/10.3390/jcm10081661 |
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author | Ramírez-López, Andrés Álvarez Román, María Teresa Monzón Manzano, Elena Acuña, Paula Arias-Salgado, Elena G. Martín Salces, Mónica Rivas Pollmar, María Isabel Jiménez Yuste, Víctor Justo Sanz, Raul García Barcenilla, Sara Cebanu, Tamara González Zorrilla, Elena Butta, Nora V. |
author_facet | Ramírez-López, Andrés Álvarez Román, María Teresa Monzón Manzano, Elena Acuña, Paula Arias-Salgado, Elena G. Martín Salces, Mónica Rivas Pollmar, María Isabel Jiménez Yuste, Víctor Justo Sanz, Raul García Barcenilla, Sara Cebanu, Tamara González Zorrilla, Elena Butta, Nora V. |
author_sort | Ramírez-López, Andrés |
collection | PubMed |
description | Loss of sialic acid from the carbohydrate side chains of platelet glycoproteins can affect platelet clearance, a proposed mechanism involved in the etiopathogenesis of immune thrombocytopaenia (ITP). We aimed to assess whether changes in platelet glycosylation in patients with ITP affected platelet counts, function, and apoptosis. This observational, prospective, and transversal study included 82 patients with chronic primary ITP and 115 healthy controls. We measured platelet activation markers and assayed platelet glycosylation and caspase activity, analysing samples using flow cytometry. Platelets from patients with ITP with a platelet count <30 × 10(3)/µL presented less sialic acid. Levels of α1,6-fucose (a glycan residue that can directly regulate antibody-dependent cellular cytotoxicity) and α-mannose (which can be recognised by mannose-binding-lectin and activate the complement pathway) were increased in the platelets from these patients. Platelet surface exposure of other glycoside residues due to sialic acid loss inversely correlated with platelet count and the ability to be activated. Moreover, loss of sialic acid induced the ingestion of platelets by human hepatome HepG2 cells. Changes in glycoside composition of glycoproteins on the platelets’ surface impaired their functional capacity and increased their apoptosis. These changes in platelet glycoside residues appeared to be related to ITP severity. |
format | Online Article Text |
id | pubmed-8069668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80696682021-04-26 The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia Ramírez-López, Andrés Álvarez Román, María Teresa Monzón Manzano, Elena Acuña, Paula Arias-Salgado, Elena G. Martín Salces, Mónica Rivas Pollmar, María Isabel Jiménez Yuste, Víctor Justo Sanz, Raul García Barcenilla, Sara Cebanu, Tamara González Zorrilla, Elena Butta, Nora V. J Clin Med Article Loss of sialic acid from the carbohydrate side chains of platelet glycoproteins can affect platelet clearance, a proposed mechanism involved in the etiopathogenesis of immune thrombocytopaenia (ITP). We aimed to assess whether changes in platelet glycosylation in patients with ITP affected platelet counts, function, and apoptosis. This observational, prospective, and transversal study included 82 patients with chronic primary ITP and 115 healthy controls. We measured platelet activation markers and assayed platelet glycosylation and caspase activity, analysing samples using flow cytometry. Platelets from patients with ITP with a platelet count <30 × 10(3)/µL presented less sialic acid. Levels of α1,6-fucose (a glycan residue that can directly regulate antibody-dependent cellular cytotoxicity) and α-mannose (which can be recognised by mannose-binding-lectin and activate the complement pathway) were increased in the platelets from these patients. Platelet surface exposure of other glycoside residues due to sialic acid loss inversely correlated with platelet count and the ability to be activated. Moreover, loss of sialic acid induced the ingestion of platelets by human hepatome HepG2 cells. Changes in glycoside composition of glycoproteins on the platelets’ surface impaired their functional capacity and increased their apoptosis. These changes in platelet glycoside residues appeared to be related to ITP severity. MDPI 2021-04-13 /pmc/articles/PMC8069668/ /pubmed/33924503 http://dx.doi.org/10.3390/jcm10081661 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ramírez-López, Andrés Álvarez Román, María Teresa Monzón Manzano, Elena Acuña, Paula Arias-Salgado, Elena G. Martín Salces, Mónica Rivas Pollmar, María Isabel Jiménez Yuste, Víctor Justo Sanz, Raul García Barcenilla, Sara Cebanu, Tamara González Zorrilla, Elena Butta, Nora V. The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
title | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
title_full | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
title_fullStr | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
title_full_unstemmed | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
title_short | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
title_sort | importance of platelet glycoside residues in the haemostasis of patients with immune thrombocytopaenia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069668/ https://www.ncbi.nlm.nih.gov/pubmed/33924503 http://dx.doi.org/10.3390/jcm10081661 |
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