Cargando…

The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro

Oxidative post-translational modifications of fibrinogen (a multifunctional blood plasma protein essential for hemostasis) are associated with the pathogenesis of cardiovascular disorders (CVDs). Prunus spinosa flower is a herbal medicine used in an adjuvant treatment of CVDs and rich in polyphenoli...

Descripción completa

Detalles Bibliográficos
Autores principales: Marchelak, Anna, Kolodziejczyk-Czepas, Joanna, Wasielewska, Paulina, Nowak, Pawel, Olszewska, Monika A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069707/
https://www.ncbi.nlm.nih.gov/pubmed/33918684
http://dx.doi.org/10.3390/antiox10040581
_version_ 1783683300247732224
author Marchelak, Anna
Kolodziejczyk-Czepas, Joanna
Wasielewska, Paulina
Nowak, Pawel
Olszewska, Monika A.
author_facet Marchelak, Anna
Kolodziejczyk-Czepas, Joanna
Wasielewska, Paulina
Nowak, Pawel
Olszewska, Monika A.
author_sort Marchelak, Anna
collection PubMed
description Oxidative post-translational modifications of fibrinogen (a multifunctional blood plasma protein essential for hemostasis) are associated with the pathogenesis of cardiovascular disorders (CVDs). Prunus spinosa flower is a herbal medicine used in an adjuvant treatment of CVDs and rich in polyphenolic antioxidants. In the present study, phytochemically standardized P. spinosa flower extracts, their primary native polyphenols and potential phenolic metabolites were evaluated in vitro for their protective effects on fibrinogen (isolated and in the human plasma matrix) using a panel of complementary methods (SDS-PAGE, western blot, C-ELISA, fluorometry, FRAP, TBARS). The results revealed that the tested analytes at in vivo relevant levels (1–5 µg/mL) considerably reduced the structural changes in the fibrinogen molecule under the oxidative stress conditions induced by peroxynitrite. In particular, they diminished the oxidation and/or nitration of amino acid residues, including tyrosine and tryptophan, as well as the formation of high molecular weight aggregates. The decrease in the levels of 3-nitrotyrosine was about 13.5–33.0% and 58.3–97.1% at 1 µg/mL and 50 µg/mL, respectively. The study indicated that low molecular weight polyphenols were crucial for the protective activity of the extracts toward fibrinogen and other human plasma components. The investigated model compounds effectively protected total plasma proteins and lipids against oxidative damage (by reducing the levels of 3-nitrotyrosine and thiobarbituric acid-reactive substances and normalizing/enhancing the non-enzymatic antioxidant capacity of plasma). The work provides insight into the role of native and metabolized polyphenols as contributory factors to the systemic activity of blackthorn flower extracts within the circulatory system.
format Online
Article
Text
id pubmed-8069707
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80697072021-04-26 The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro Marchelak, Anna Kolodziejczyk-Czepas, Joanna Wasielewska, Paulina Nowak, Pawel Olszewska, Monika A. Antioxidants (Basel) Article Oxidative post-translational modifications of fibrinogen (a multifunctional blood plasma protein essential for hemostasis) are associated with the pathogenesis of cardiovascular disorders (CVDs). Prunus spinosa flower is a herbal medicine used in an adjuvant treatment of CVDs and rich in polyphenolic antioxidants. In the present study, phytochemically standardized P. spinosa flower extracts, their primary native polyphenols and potential phenolic metabolites were evaluated in vitro for their protective effects on fibrinogen (isolated and in the human plasma matrix) using a panel of complementary methods (SDS-PAGE, western blot, C-ELISA, fluorometry, FRAP, TBARS). The results revealed that the tested analytes at in vivo relevant levels (1–5 µg/mL) considerably reduced the structural changes in the fibrinogen molecule under the oxidative stress conditions induced by peroxynitrite. In particular, they diminished the oxidation and/or nitration of amino acid residues, including tyrosine and tryptophan, as well as the formation of high molecular weight aggregates. The decrease in the levels of 3-nitrotyrosine was about 13.5–33.0% and 58.3–97.1% at 1 µg/mL and 50 µg/mL, respectively. The study indicated that low molecular weight polyphenols were crucial for the protective activity of the extracts toward fibrinogen and other human plasma components. The investigated model compounds effectively protected total plasma proteins and lipids against oxidative damage (by reducing the levels of 3-nitrotyrosine and thiobarbituric acid-reactive substances and normalizing/enhancing the non-enzymatic antioxidant capacity of plasma). The work provides insight into the role of native and metabolized polyphenols as contributory factors to the systemic activity of blackthorn flower extracts within the circulatory system. MDPI 2021-04-09 /pmc/articles/PMC8069707/ /pubmed/33918684 http://dx.doi.org/10.3390/antiox10040581 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marchelak, Anna
Kolodziejczyk-Czepas, Joanna
Wasielewska, Paulina
Nowak, Pawel
Olszewska, Monika A.
The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro
title The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro
title_full The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro
title_fullStr The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro
title_full_unstemmed The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro
title_short The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro
title_sort effects of prunus spinosa l. flower extracts, model polyphenols and phenolic metabolites on oxidative/nitrative modifications of human plasma components with particular emphasis on fibrinogen in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069707/
https://www.ncbi.nlm.nih.gov/pubmed/33918684
http://dx.doi.org/10.3390/antiox10040581
work_keys_str_mv AT marchelakanna theeffectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT kolodziejczykczepasjoanna theeffectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT wasielewskapaulina theeffectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT nowakpawel theeffectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT olszewskamonikaa theeffectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT marchelakanna effectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT kolodziejczykczepasjoanna effectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT wasielewskapaulina effectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT nowakpawel effectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro
AT olszewskamonikaa effectsofprunusspinosalflowerextractsmodelpolyphenolsandphenolicmetabolitesonoxidativenitrativemodificationsofhumanplasmacomponentswithparticularemphasisonfibrinogeninvitro