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Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives

Some of the major impacts of climate change are expected in regions where drought stress is already an issue. Grain legumes are generally drought susceptible. However, tepary bean and its wild relatives within Phaseolus acutifolius or P. parvifolius are from arid areas between Mexico and the United...

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Autores principales: Buitrago-Bitar, María A., Cortés, Andrés J., López-Hernández, Felipe, Londoño-Caicedo, Jorge M., Muñoz-Florez, Jaime E., Muñoz, L. Carmenza, Blair, Matthew Wohlgemuth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070098/
https://www.ncbi.nlm.nih.gov/pubmed/33921270
http://dx.doi.org/10.3390/genes12040556
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author Buitrago-Bitar, María A.
Cortés, Andrés J.
López-Hernández, Felipe
Londoño-Caicedo, Jorge M.
Muñoz-Florez, Jaime E.
Muñoz, L. Carmenza
Blair, Matthew Wohlgemuth
author_facet Buitrago-Bitar, María A.
Cortés, Andrés J.
López-Hernández, Felipe
Londoño-Caicedo, Jorge M.
Muñoz-Florez, Jaime E.
Muñoz, L. Carmenza
Blair, Matthew Wohlgemuth
author_sort Buitrago-Bitar, María A.
collection PubMed
description Some of the major impacts of climate change are expected in regions where drought stress is already an issue. Grain legumes are generally drought susceptible. However, tepary bean and its wild relatives within Phaseolus acutifolius or P. parvifolius are from arid areas between Mexico and the United States. Therefore, we hypothesize that these bean accessions have diversity signals indicative of adaptation to drought at key candidate genes such as: Asr2, Dreb2B, and ERECTA. By sequencing alleles of these genes and comparing to estimates of drought tolerance indices from climate data for the collection site of geo-referenced, tepary bean accessions, we determined the genotype x environmental association (GEA) of each gene. Diversity analysis found that cultivated and wild P. acutifolius were intermingled with var. tenuifolius and P. parvifolius, signifying that allele diversity was ample in the wild and cultivated clade over a broad sense (sensu lato) evaluation. Genes Dreb2B and ERECTA harbored signatures of directional selection, represented by six SNPs correlated with the environmental drought indices. This suggests that wild tepary bean is a reservoir of novel alleles at genes for drought tolerance, as expected for a species that originated in arid environments. Our study corroborated that candidate gene approach was effective for marker validation across a broad genetic base of wild tepary accessions.
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spelling pubmed-80700982021-04-26 Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives Buitrago-Bitar, María A. Cortés, Andrés J. López-Hernández, Felipe Londoño-Caicedo, Jorge M. Muñoz-Florez, Jaime E. Muñoz, L. Carmenza Blair, Matthew Wohlgemuth Genes (Basel) Article Some of the major impacts of climate change are expected in regions where drought stress is already an issue. Grain legumes are generally drought susceptible. However, tepary bean and its wild relatives within Phaseolus acutifolius or P. parvifolius are from arid areas between Mexico and the United States. Therefore, we hypothesize that these bean accessions have diversity signals indicative of adaptation to drought at key candidate genes such as: Asr2, Dreb2B, and ERECTA. By sequencing alleles of these genes and comparing to estimates of drought tolerance indices from climate data for the collection site of geo-referenced, tepary bean accessions, we determined the genotype x environmental association (GEA) of each gene. Diversity analysis found that cultivated and wild P. acutifolius were intermingled with var. tenuifolius and P. parvifolius, signifying that allele diversity was ample in the wild and cultivated clade over a broad sense (sensu lato) evaluation. Genes Dreb2B and ERECTA harbored signatures of directional selection, represented by six SNPs correlated with the environmental drought indices. This suggests that wild tepary bean is a reservoir of novel alleles at genes for drought tolerance, as expected for a species that originated in arid environments. Our study corroborated that candidate gene approach was effective for marker validation across a broad genetic base of wild tepary accessions. MDPI 2021-04-12 /pmc/articles/PMC8070098/ /pubmed/33921270 http://dx.doi.org/10.3390/genes12040556 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Buitrago-Bitar, María A.
Cortés, Andrés J.
López-Hernández, Felipe
Londoño-Caicedo, Jorge M.
Muñoz-Florez, Jaime E.
Muñoz, L. Carmenza
Blair, Matthew Wohlgemuth
Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives
title Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives
title_full Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives
title_fullStr Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives
title_full_unstemmed Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives
title_short Allelic Diversity at Abiotic Stress Responsive Genes in Relationship to Ecological Drought Indices for Cultivated Tepary Bean, Phaseolus acutifolius A. Gray, and Its Wild Relatives
title_sort allelic diversity at abiotic stress responsive genes in relationship to ecological drought indices for cultivated tepary bean, phaseolus acutifolius a. gray, and its wild relatives
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070098/
https://www.ncbi.nlm.nih.gov/pubmed/33921270
http://dx.doi.org/10.3390/genes12040556
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