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Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland

Orchidaceae is one of the largest and the most widespread plant families with many species threatened with extinction. However, only about 1.5% of orchids’ genome sizes have been known so far. The aim of this study was to estimate the genome size of 15 species and one infraspecific taxon of endanger...

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Autores principales: Rewers, Monika, Jedrzejczyk, Iwona, Rewicz, Agnieszka, Jakubska-Busse, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070121/
https://www.ncbi.nlm.nih.gov/pubmed/33924526
http://dx.doi.org/10.3390/genes12040563
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author Rewers, Monika
Jedrzejczyk, Iwona
Rewicz, Agnieszka
Jakubska-Busse, Anna
author_facet Rewers, Monika
Jedrzejczyk, Iwona
Rewicz, Agnieszka
Jakubska-Busse, Anna
author_sort Rewers, Monika
collection PubMed
description Orchidaceae is one of the largest and the most widespread plant families with many species threatened with extinction. However, only about 1.5% of orchids’ genome sizes have been known so far. The aim of this study was to estimate the genome size of 15 species and one infraspecific taxon of endangered and protected orchids growing wild in Poland to assess their variability and develop additional criterion useful in orchid species identification and characterization. Flow cytometric genome size estimation revealed that investigated orchid species possessed intermediate, large, and very large genomes. The smallest 2C DNA content possessed Liparis loeselii (14.15 pg), while the largest Cypripedium calceolus (82.10 pg). It was confirmed that the genome size is characteristic to the subfamily. Additionally, for four species Epipactis albensis, Ophrys insectifera, Orchis mascula, Orchis militaris and one infraspecific taxon, Epipactis purpurata f. chlorophylla the 2C DNA content has been estimated for the first time. Genome size estimation by flow cytometry proved to be a useful auxiliary method for quick orchid species identification and characterization.
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spelling pubmed-80701212021-04-26 Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland Rewers, Monika Jedrzejczyk, Iwona Rewicz, Agnieszka Jakubska-Busse, Anna Genes (Basel) Communication Orchidaceae is one of the largest and the most widespread plant families with many species threatened with extinction. However, only about 1.5% of orchids’ genome sizes have been known so far. The aim of this study was to estimate the genome size of 15 species and one infraspecific taxon of endangered and protected orchids growing wild in Poland to assess their variability and develop additional criterion useful in orchid species identification and characterization. Flow cytometric genome size estimation revealed that investigated orchid species possessed intermediate, large, and very large genomes. The smallest 2C DNA content possessed Liparis loeselii (14.15 pg), while the largest Cypripedium calceolus (82.10 pg). It was confirmed that the genome size is characteristic to the subfamily. Additionally, for four species Epipactis albensis, Ophrys insectifera, Orchis mascula, Orchis militaris and one infraspecific taxon, Epipactis purpurata f. chlorophylla the 2C DNA content has been estimated for the first time. Genome size estimation by flow cytometry proved to be a useful auxiliary method for quick orchid species identification and characterization. MDPI 2021-04-13 /pmc/articles/PMC8070121/ /pubmed/33924526 http://dx.doi.org/10.3390/genes12040563 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Rewers, Monika
Jedrzejczyk, Iwona
Rewicz, Agnieszka
Jakubska-Busse, Anna
Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland
title Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland
title_full Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland
title_fullStr Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland
title_full_unstemmed Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland
title_short Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland
title_sort genome size diversity in rare, endangered, and protected orchids in poland
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070121/
https://www.ncbi.nlm.nih.gov/pubmed/33924526
http://dx.doi.org/10.3390/genes12040563
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