Cargando…
Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland
Orchidaceae is one of the largest and the most widespread plant families with many species threatened with extinction. However, only about 1.5% of orchids’ genome sizes have been known so far. The aim of this study was to estimate the genome size of 15 species and one infraspecific taxon of endanger...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070121/ https://www.ncbi.nlm.nih.gov/pubmed/33924526 http://dx.doi.org/10.3390/genes12040563 |
_version_ | 1783683396522737664 |
---|---|
author | Rewers, Monika Jedrzejczyk, Iwona Rewicz, Agnieszka Jakubska-Busse, Anna |
author_facet | Rewers, Monika Jedrzejczyk, Iwona Rewicz, Agnieszka Jakubska-Busse, Anna |
author_sort | Rewers, Monika |
collection | PubMed |
description | Orchidaceae is one of the largest and the most widespread plant families with many species threatened with extinction. However, only about 1.5% of orchids’ genome sizes have been known so far. The aim of this study was to estimate the genome size of 15 species and one infraspecific taxon of endangered and protected orchids growing wild in Poland to assess their variability and develop additional criterion useful in orchid species identification and characterization. Flow cytometric genome size estimation revealed that investigated orchid species possessed intermediate, large, and very large genomes. The smallest 2C DNA content possessed Liparis loeselii (14.15 pg), while the largest Cypripedium calceolus (82.10 pg). It was confirmed that the genome size is characteristic to the subfamily. Additionally, for four species Epipactis albensis, Ophrys insectifera, Orchis mascula, Orchis militaris and one infraspecific taxon, Epipactis purpurata f. chlorophylla the 2C DNA content has been estimated for the first time. Genome size estimation by flow cytometry proved to be a useful auxiliary method for quick orchid species identification and characterization. |
format | Online Article Text |
id | pubmed-8070121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80701212021-04-26 Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland Rewers, Monika Jedrzejczyk, Iwona Rewicz, Agnieszka Jakubska-Busse, Anna Genes (Basel) Communication Orchidaceae is one of the largest and the most widespread plant families with many species threatened with extinction. However, only about 1.5% of orchids’ genome sizes have been known so far. The aim of this study was to estimate the genome size of 15 species and one infraspecific taxon of endangered and protected orchids growing wild in Poland to assess their variability and develop additional criterion useful in orchid species identification and characterization. Flow cytometric genome size estimation revealed that investigated orchid species possessed intermediate, large, and very large genomes. The smallest 2C DNA content possessed Liparis loeselii (14.15 pg), while the largest Cypripedium calceolus (82.10 pg). It was confirmed that the genome size is characteristic to the subfamily. Additionally, for four species Epipactis albensis, Ophrys insectifera, Orchis mascula, Orchis militaris and one infraspecific taxon, Epipactis purpurata f. chlorophylla the 2C DNA content has been estimated for the first time. Genome size estimation by flow cytometry proved to be a useful auxiliary method for quick orchid species identification and characterization. MDPI 2021-04-13 /pmc/articles/PMC8070121/ /pubmed/33924526 http://dx.doi.org/10.3390/genes12040563 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Rewers, Monika Jedrzejczyk, Iwona Rewicz, Agnieszka Jakubska-Busse, Anna Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland |
title | Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland |
title_full | Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland |
title_fullStr | Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland |
title_full_unstemmed | Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland |
title_short | Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland |
title_sort | genome size diversity in rare, endangered, and protected orchids in poland |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070121/ https://www.ncbi.nlm.nih.gov/pubmed/33924526 http://dx.doi.org/10.3390/genes12040563 |
work_keys_str_mv | AT rewersmonika genomesizediversityinrareendangeredandprotectedorchidsinpoland AT jedrzejczykiwona genomesizediversityinrareendangeredandprotectedorchidsinpoland AT rewiczagnieszka genomesizediversityinrareendangeredandprotectedorchidsinpoland AT jakubskabusseanna genomesizediversityinrareendangeredandprotectedorchidsinpoland |