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The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts

Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinn...

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Autores principales: Filova, Elena, Blanquer, Andreu, Knitlova, Jarmila, Plencner, Martin, Jencova, Vera, Koprivova, Barbora, Lisnenko, Maxim, Kostakova, Eva Kuzelova, Prochazkova, Renata, Bacakova, Lucie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070234/
https://www.ncbi.nlm.nih.gov/pubmed/33924537
http://dx.doi.org/10.3390/nano11040995
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author Filova, Elena
Blanquer, Andreu
Knitlova, Jarmila
Plencner, Martin
Jencova, Vera
Koprivova, Barbora
Lisnenko, Maxim
Kostakova, Eva Kuzelova
Prochazkova, Renata
Bacakova, Lucie
author_facet Filova, Elena
Blanquer, Andreu
Knitlova, Jarmila
Plencner, Martin
Jencova, Vera
Koprivova, Barbora
Lisnenko, Maxim
Kostakova, Eva Kuzelova
Prochazkova, Renata
Bacakova, Lucie
author_sort Filova, Elena
collection PubMed
description Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinning technique using poly(vinyl alcohol) (PVA) with a high molecular weight and with a high degree of hydrolysis with the incorporated PL (10% w/w). The morphology, wettability and protein release from the nanofibers was then assessed from the resulting composite PVA–PL nanomats. The bioactivity of the PVA–PL nanomats was proved in vitro using HaCaT keratinocytes, human saphenous endothelial cells (HSVECs) and 3T3 fibroblasts. The PVA–PL supported cell adhesion, proliferation, and viability. The improved phenotypic maturation of the HaCaT cells due to the PVA–PL was manifested via the formation of intermediate filaments positive for cytokeratin 10. The PVA–PL enhanced both the synthesis of the von Willebrand factor via HSVECs and HSVECs chemotaxis through membranes with 8 µm-sized pores. These results indicated the favorable effects of the PVA–PL nanomats on the three cell types involved in the wound healing process, and established PVA–PL nanomats as a promising candidate for further evaluation with respect to in vivo experiments.
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spelling pubmed-80702342021-04-26 The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts Filova, Elena Blanquer, Andreu Knitlova, Jarmila Plencner, Martin Jencova, Vera Koprivova, Barbora Lisnenko, Maxim Kostakova, Eva Kuzelova Prochazkova, Renata Bacakova, Lucie Nanomaterials (Basel) Article Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinning technique using poly(vinyl alcohol) (PVA) with a high molecular weight and with a high degree of hydrolysis with the incorporated PL (10% w/w). The morphology, wettability and protein release from the nanofibers was then assessed from the resulting composite PVA–PL nanomats. The bioactivity of the PVA–PL nanomats was proved in vitro using HaCaT keratinocytes, human saphenous endothelial cells (HSVECs) and 3T3 fibroblasts. The PVA–PL supported cell adhesion, proliferation, and viability. The improved phenotypic maturation of the HaCaT cells due to the PVA–PL was manifested via the formation of intermediate filaments positive for cytokeratin 10. The PVA–PL enhanced both the synthesis of the von Willebrand factor via HSVECs and HSVECs chemotaxis through membranes with 8 µm-sized pores. These results indicated the favorable effects of the PVA–PL nanomats on the three cell types involved in the wound healing process, and established PVA–PL nanomats as a promising candidate for further evaluation with respect to in vivo experiments. MDPI 2021-04-13 /pmc/articles/PMC8070234/ /pubmed/33924537 http://dx.doi.org/10.3390/nano11040995 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Filova, Elena
Blanquer, Andreu
Knitlova, Jarmila
Plencner, Martin
Jencova, Vera
Koprivova, Barbora
Lisnenko, Maxim
Kostakova, Eva Kuzelova
Prochazkova, Renata
Bacakova, Lucie
The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
title The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
title_full The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
title_fullStr The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
title_full_unstemmed The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
title_short The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
title_sort effect of the controlled release of platelet lysate from pva nanomats on keratinocytes, endothelial cells and fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070234/
https://www.ncbi.nlm.nih.gov/pubmed/33924537
http://dx.doi.org/10.3390/nano11040995
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