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Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds

Chronic infections are considered one of the most severe problems in skin wounds, and bacteria are present in over 90% of chronic wounds. Pseudomonas aeruginosa is frequently isolated from chronic wounds and is thought to be a cause of delayed wound healing. Invariant natural killer T (iNKT) cells,...

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Autores principales: Tanno, Hiromasa, Kanno, Emi, Sato, Suzuna, Asao, Yu, Shimono, Mizuki, Kurosaka, Shiho, Oikawa, Yukari, Ishi, Shinyo, Shoji, Miki, Sato, Ko, Kasamatsu, Jun, Miyasaka, Tomomitsu, Yamamoto, Hideki, Ishii, Keiko, Imai, Yoshimichi, Tachi, Masahiro, Kawakami, Kazuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070359/
https://www.ncbi.nlm.nih.gov/pubmed/33920301
http://dx.doi.org/10.3390/ijms22083931
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author Tanno, Hiromasa
Kanno, Emi
Sato, Suzuna
Asao, Yu
Shimono, Mizuki
Kurosaka, Shiho
Oikawa, Yukari
Ishi, Shinyo
Shoji, Miki
Sato, Ko
Kasamatsu, Jun
Miyasaka, Tomomitsu
Yamamoto, Hideki
Ishii, Keiko
Imai, Yoshimichi
Tachi, Masahiro
Kawakami, Kazuyoshi
author_facet Tanno, Hiromasa
Kanno, Emi
Sato, Suzuna
Asao, Yu
Shimono, Mizuki
Kurosaka, Shiho
Oikawa, Yukari
Ishi, Shinyo
Shoji, Miki
Sato, Ko
Kasamatsu, Jun
Miyasaka, Tomomitsu
Yamamoto, Hideki
Ishii, Keiko
Imai, Yoshimichi
Tachi, Masahiro
Kawakami, Kazuyoshi
author_sort Tanno, Hiromasa
collection PubMed
description Chronic infections are considered one of the most severe problems in skin wounds, and bacteria are present in over 90% of chronic wounds. Pseudomonas aeruginosa is frequently isolated from chronic wounds and is thought to be a cause of delayed wound healing. Invariant natural killer T (iNKT) cells, unique lymphocytes with a potent regulatory ability in various inflammatory responses, accelerate the wound healing process. In the present study, we investigated the contribution of iNKT cells in the host defense against P. aeruginosa inoculation at the wound sites. We analyzed the re-epithelialization, bacterial load, accumulation of leukocytes, and production of cytokines and antimicrobial peptides. In iNKT cell–deficient (Jα18KO) mice, re-epithelialization was significantly decreased, and the number of live colonies was significantly increased, when compared with those in wild-type (WT) mice on day 7. IL-17A, and IL-22 production was significantly lower in Jα18KO mice than in WT mice on day 5. Furthermore, the administration of α-galactosylceramide (α-GalCer), a specific activator of iNKT cells, led to enhanced host protection, as shown by reduced bacterial load, and to increased production of IL-22, IL-23, and S100A9 compared that of with WT mice. These results suggest that iNKT cells promote P. aeruginosa clearance during skin wound healing.
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spelling pubmed-80703592021-04-26 Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds Tanno, Hiromasa Kanno, Emi Sato, Suzuna Asao, Yu Shimono, Mizuki Kurosaka, Shiho Oikawa, Yukari Ishi, Shinyo Shoji, Miki Sato, Ko Kasamatsu, Jun Miyasaka, Tomomitsu Yamamoto, Hideki Ishii, Keiko Imai, Yoshimichi Tachi, Masahiro Kawakami, Kazuyoshi Int J Mol Sci Article Chronic infections are considered one of the most severe problems in skin wounds, and bacteria are present in over 90% of chronic wounds. Pseudomonas aeruginosa is frequently isolated from chronic wounds and is thought to be a cause of delayed wound healing. Invariant natural killer T (iNKT) cells, unique lymphocytes with a potent regulatory ability in various inflammatory responses, accelerate the wound healing process. In the present study, we investigated the contribution of iNKT cells in the host defense against P. aeruginosa inoculation at the wound sites. We analyzed the re-epithelialization, bacterial load, accumulation of leukocytes, and production of cytokines and antimicrobial peptides. In iNKT cell–deficient (Jα18KO) mice, re-epithelialization was significantly decreased, and the number of live colonies was significantly increased, when compared with those in wild-type (WT) mice on day 7. IL-17A, and IL-22 production was significantly lower in Jα18KO mice than in WT mice on day 5. Furthermore, the administration of α-galactosylceramide (α-GalCer), a specific activator of iNKT cells, led to enhanced host protection, as shown by reduced bacterial load, and to increased production of IL-22, IL-23, and S100A9 compared that of with WT mice. These results suggest that iNKT cells promote P. aeruginosa clearance during skin wound healing. MDPI 2021-04-10 /pmc/articles/PMC8070359/ /pubmed/33920301 http://dx.doi.org/10.3390/ijms22083931 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tanno, Hiromasa
Kanno, Emi
Sato, Suzuna
Asao, Yu
Shimono, Mizuki
Kurosaka, Shiho
Oikawa, Yukari
Ishi, Shinyo
Shoji, Miki
Sato, Ko
Kasamatsu, Jun
Miyasaka, Tomomitsu
Yamamoto, Hideki
Ishii, Keiko
Imai, Yoshimichi
Tachi, Masahiro
Kawakami, Kazuyoshi
Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds
title Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds
title_full Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds
title_fullStr Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds
title_full_unstemmed Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds
title_short Contribution of Invariant Natural Killer T Cells to the Clearance of Pseudomonas aeruginosa from Skin Wounds
title_sort contribution of invariant natural killer t cells to the clearance of pseudomonas aeruginosa from skin wounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070359/
https://www.ncbi.nlm.nih.gov/pubmed/33920301
http://dx.doi.org/10.3390/ijms22083931
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