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Potential Non-Invasive Biomarkers for Early Diagnosis of Oral Squamous Cell Carcinoma

This study aimed to investigate the role of a panel of salivary cytokines as biomarkers for early detection oral squamous cell carcinoma (OSCC), comparing their levels among healthy individuals, patients with oral leukoplakia (OL), and malignant lesions. Cytokine profiling analysis performed in a mi...

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Autores principales: Dikova, Valentina, Jantus-Lewintre, Eloisa, Bagan, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070511/
https://www.ncbi.nlm.nih.gov/pubmed/33924500
http://dx.doi.org/10.3390/jcm10081658
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author Dikova, Valentina
Jantus-Lewintre, Eloisa
Bagan, Jose
author_facet Dikova, Valentina
Jantus-Lewintre, Eloisa
Bagan, Jose
author_sort Dikova, Valentina
collection PubMed
description This study aimed to investigate the role of a panel of salivary cytokines as biomarkers for early detection oral squamous cell carcinoma (OSCC), comparing their levels among healthy individuals, patients with oral leukoplakia (OL), and malignant lesions. Cytokine profiling analysis performed in a minimally invasive sample was correlated with clinicopathological variables in our patient cohorts. Unstimulated saliva was obtained from subjects with OSCC at early (n = 33) and advanced (n = 33) disease, OL with homogeneous (n = 33) and proliferative verrucous (n = 33) clinical presentations, and healthy controls (n = 25). Salivary IL-1α, IL-6, IL-8, IP-10, MCP-1, TNF-α, HCC-1, and PF-4 levels were analyzed by a sensitive bead-based multiplex immunoassay. Mean levels of IL-6, IL-8, TNF-α, HCC-1, MCP-1, and PF-4 differed significantly between OSCC, OL, and control saliva (p < 0.05). We found notably higher IL-6 and TNF-α in advanced compared to early OSCC stages. The area under the curve (AUC) for OSCC vs. control was greater than 0.8 for IL-6, IL-8, TNF-α, and HCC-1, and greater than 0.7 for PF-4. The presence of neck metastases (NM) was associated with increased IL-6 and TNF-α levels. Our findings suggest that salivary IL-6, IL-8, TNF-α, HCC-1, and PF-4 may discriminate between OSCC, OL, and healthy controls. IL-6 and TNF-α may indicate OSCC progression, being distinctive in the presence of NM.
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spelling pubmed-80705112021-04-26 Potential Non-Invasive Biomarkers for Early Diagnosis of Oral Squamous Cell Carcinoma Dikova, Valentina Jantus-Lewintre, Eloisa Bagan, Jose J Clin Med Article This study aimed to investigate the role of a panel of salivary cytokines as biomarkers for early detection oral squamous cell carcinoma (OSCC), comparing their levels among healthy individuals, patients with oral leukoplakia (OL), and malignant lesions. Cytokine profiling analysis performed in a minimally invasive sample was correlated with clinicopathological variables in our patient cohorts. Unstimulated saliva was obtained from subjects with OSCC at early (n = 33) and advanced (n = 33) disease, OL with homogeneous (n = 33) and proliferative verrucous (n = 33) clinical presentations, and healthy controls (n = 25). Salivary IL-1α, IL-6, IL-8, IP-10, MCP-1, TNF-α, HCC-1, and PF-4 levels were analyzed by a sensitive bead-based multiplex immunoassay. Mean levels of IL-6, IL-8, TNF-α, HCC-1, MCP-1, and PF-4 differed significantly between OSCC, OL, and control saliva (p < 0.05). We found notably higher IL-6 and TNF-α in advanced compared to early OSCC stages. The area under the curve (AUC) for OSCC vs. control was greater than 0.8 for IL-6, IL-8, TNF-α, and HCC-1, and greater than 0.7 for PF-4. The presence of neck metastases (NM) was associated with increased IL-6 and TNF-α levels. Our findings suggest that salivary IL-6, IL-8, TNF-α, HCC-1, and PF-4 may discriminate between OSCC, OL, and healthy controls. IL-6 and TNF-α may indicate OSCC progression, being distinctive in the presence of NM. MDPI 2021-04-13 /pmc/articles/PMC8070511/ /pubmed/33924500 http://dx.doi.org/10.3390/jcm10081658 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dikova, Valentina
Jantus-Lewintre, Eloisa
Bagan, Jose
Potential Non-Invasive Biomarkers for Early Diagnosis of Oral Squamous Cell Carcinoma
title Potential Non-Invasive Biomarkers for Early Diagnosis of Oral Squamous Cell Carcinoma
title_full Potential Non-Invasive Biomarkers for Early Diagnosis of Oral Squamous Cell Carcinoma
title_fullStr Potential Non-Invasive Biomarkers for Early Diagnosis of Oral Squamous Cell Carcinoma
title_full_unstemmed Potential Non-Invasive Biomarkers for Early Diagnosis of Oral Squamous Cell Carcinoma
title_short Potential Non-Invasive Biomarkers for Early Diagnosis of Oral Squamous Cell Carcinoma
title_sort potential non-invasive biomarkers for early diagnosis of oral squamous cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070511/
https://www.ncbi.nlm.nih.gov/pubmed/33924500
http://dx.doi.org/10.3390/jcm10081658
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