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Gut Microbiome in a Russian Cohort of Pre- and Post-Cholecystectomy Female Patients

The last decade saw extensive studies of the human gut microbiome and its relationship to specific diseases, including gallstone disease (GSD). The information about the gut microbiome in GSD-afflicted Russian patients is scarce, despite the increasing GSD incidence worldwide. Although the gut micro...

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Autores principales: Grigor’eva, Irina, Romanova, Tatiana, Naumova, Natalia, Alikina, Tatiana, Kuznetsov, Alexey, Kabilov, Marsel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070538/
https://www.ncbi.nlm.nih.gov/pubmed/33921449
http://dx.doi.org/10.3390/jpm11040294
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author Grigor’eva, Irina
Romanova, Tatiana
Naumova, Natalia
Alikina, Tatiana
Kuznetsov, Alexey
Kabilov, Marsel
author_facet Grigor’eva, Irina
Romanova, Tatiana
Naumova, Natalia
Alikina, Tatiana
Kuznetsov, Alexey
Kabilov, Marsel
author_sort Grigor’eva, Irina
collection PubMed
description The last decade saw extensive studies of the human gut microbiome and its relationship to specific diseases, including gallstone disease (GSD). The information about the gut microbiome in GSD-afflicted Russian patients is scarce, despite the increasing GSD incidence worldwide. Although the gut microbiota was described in some GSD cohorts, little is known regarding the gut microbiome before and after cholecystectomy (CCE). By using Illumina MiSeq sequencing of 16S rRNA gene amplicons, we inventoried the fecal bacteriobiome composition and structure in GSD-afflicted females, seeking to reveal associations with age, BMI and some blood biochemistry. Overall, 11 bacterial phyla were identified, containing 916 operational taxonomic units (OTUs). The fecal bacteriobiome was dominated by Firmicutes (66% relative abundance), followed by Bacteroidetes (19%), Actinobacteria (8%) and Proteobacteria (4%) phyla. Most (97%) of the OTUs were minor or rare species with ≤1% relative abundance. Prevotella and Enterocossus were linked to blood bilirubin. Some taxa had differential pre- and post-CCE abundance, despite the very short time (1–3 days) elapsed after CCE. The detailed description of the bacteriobiome in pre-CCE female patients suggests bacterial foci for further research to elucidate the gut microbiota and GSD relationship and has potentially important biological and medical implications regarding gut bacteria involvement in the increased GSD incidence rate in females.
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spelling pubmed-80705382021-04-26 Gut Microbiome in a Russian Cohort of Pre- and Post-Cholecystectomy Female Patients Grigor’eva, Irina Romanova, Tatiana Naumova, Natalia Alikina, Tatiana Kuznetsov, Alexey Kabilov, Marsel J Pers Med Article The last decade saw extensive studies of the human gut microbiome and its relationship to specific diseases, including gallstone disease (GSD). The information about the gut microbiome in GSD-afflicted Russian patients is scarce, despite the increasing GSD incidence worldwide. Although the gut microbiota was described in some GSD cohorts, little is known regarding the gut microbiome before and after cholecystectomy (CCE). By using Illumina MiSeq sequencing of 16S rRNA gene amplicons, we inventoried the fecal bacteriobiome composition and structure in GSD-afflicted females, seeking to reveal associations with age, BMI and some blood biochemistry. Overall, 11 bacterial phyla were identified, containing 916 operational taxonomic units (OTUs). The fecal bacteriobiome was dominated by Firmicutes (66% relative abundance), followed by Bacteroidetes (19%), Actinobacteria (8%) and Proteobacteria (4%) phyla. Most (97%) of the OTUs were minor or rare species with ≤1% relative abundance. Prevotella and Enterocossus were linked to blood bilirubin. Some taxa had differential pre- and post-CCE abundance, despite the very short time (1–3 days) elapsed after CCE. The detailed description of the bacteriobiome in pre-CCE female patients suggests bacterial foci for further research to elucidate the gut microbiota and GSD relationship and has potentially important biological and medical implications regarding gut bacteria involvement in the increased GSD incidence rate in females. MDPI 2021-04-12 /pmc/articles/PMC8070538/ /pubmed/33921449 http://dx.doi.org/10.3390/jpm11040294 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grigor’eva, Irina
Romanova, Tatiana
Naumova, Natalia
Alikina, Tatiana
Kuznetsov, Alexey
Kabilov, Marsel
Gut Microbiome in a Russian Cohort of Pre- and Post-Cholecystectomy Female Patients
title Gut Microbiome in a Russian Cohort of Pre- and Post-Cholecystectomy Female Patients
title_full Gut Microbiome in a Russian Cohort of Pre- and Post-Cholecystectomy Female Patients
title_fullStr Gut Microbiome in a Russian Cohort of Pre- and Post-Cholecystectomy Female Patients
title_full_unstemmed Gut Microbiome in a Russian Cohort of Pre- and Post-Cholecystectomy Female Patients
title_short Gut Microbiome in a Russian Cohort of Pre- and Post-Cholecystectomy Female Patients
title_sort gut microbiome in a russian cohort of pre- and post-cholecystectomy female patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070538/
https://www.ncbi.nlm.nih.gov/pubmed/33921449
http://dx.doi.org/10.3390/jpm11040294
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