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The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms
The effect of norepinephrine (“NE”) on Gram-negative bacteria is well characterized; however, little is known about the impact of NE on cutaneous Gram-positive skin residents, especially staphylococci. In this study, the impact of NE on monospecies and dual-species biofilms of Staphylococcus epiderm...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070549/ https://www.ncbi.nlm.nih.gov/pubmed/33924447 http://dx.doi.org/10.3390/microorganisms9040820 |
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author | Mart’yanov, Sergey Vladislavovich Botchkova, Ekaterina Alexandrovna Plakunov, Vladimir Konstantinovich Gannesen, Andrei Vladislavovich |
author_facet | Mart’yanov, Sergey Vladislavovich Botchkova, Ekaterina Alexandrovna Plakunov, Vladimir Konstantinovich Gannesen, Andrei Vladislavovich |
author_sort | Mart’yanov, Sergey Vladislavovich |
collection | PubMed |
description | The effect of norepinephrine (“NE”) on Gram-negative bacteria is well characterized; however, little is known about the impact of NE on cutaneous Gram-positive skin residents, especially staphylococci. In this study, the impact of NE on monospecies and dual-species biofilms of Staphylococcus epidermidis and S. aureus model strains was investigated for the first time. Biofilms were grown in two different models (on polytetrafluoroethylene (“PTFE”) cubes and glass microfiber filters (“GMFFs”)) and additionally kinetic measurements of bacterial growth was performed. We have shown that NE can affect the biofilm formation of both species with a strong dependence on aerobic or anaerobic culture conditions in different models. It was shown that S. epidermidis suppresses S. aureus growth in dual-species biofilms and that NE can accelerate this process, contributing to the competitive behavior of staphylococci. |
format | Online Article Text |
id | pubmed-8070549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80705492021-04-26 The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms Mart’yanov, Sergey Vladislavovich Botchkova, Ekaterina Alexandrovna Plakunov, Vladimir Konstantinovich Gannesen, Andrei Vladislavovich Microorganisms Communication The effect of norepinephrine (“NE”) on Gram-negative bacteria is well characterized; however, little is known about the impact of NE on cutaneous Gram-positive skin residents, especially staphylococci. In this study, the impact of NE on monospecies and dual-species biofilms of Staphylococcus epidermidis and S. aureus model strains was investigated for the first time. Biofilms were grown in two different models (on polytetrafluoroethylene (“PTFE”) cubes and glass microfiber filters (“GMFFs”)) and additionally kinetic measurements of bacterial growth was performed. We have shown that NE can affect the biofilm formation of both species with a strong dependence on aerobic or anaerobic culture conditions in different models. It was shown that S. epidermidis suppresses S. aureus growth in dual-species biofilms and that NE can accelerate this process, contributing to the competitive behavior of staphylococci. MDPI 2021-04-13 /pmc/articles/PMC8070549/ /pubmed/33924447 http://dx.doi.org/10.3390/microorganisms9040820 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Mart’yanov, Sergey Vladislavovich Botchkova, Ekaterina Alexandrovna Plakunov, Vladimir Konstantinovich Gannesen, Andrei Vladislavovich The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms |
title | The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms |
title_full | The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms |
title_fullStr | The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms |
title_full_unstemmed | The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms |
title_short | The Impact of Norepinephrine on Mono-Species and Dual-Species Staphylococcal Biofilms |
title_sort | impact of norepinephrine on mono-species and dual-species staphylococcal biofilms |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070549/ https://www.ncbi.nlm.nih.gov/pubmed/33924447 http://dx.doi.org/10.3390/microorganisms9040820 |
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