COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins

COPD-derived fibroblasts have increased cellular senescence. Senescent cell accumulation can induce tissue dysfunction by their senescence-associated secretory phenotype (SASP). We aimed to determine the SASP of senescent fibroblasts and COPD-derived lung fibroblasts, including severe, early-onset (...

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Autores principales: Woldhuis, Roy R, Heijink, Irene H, van den Berge, Maarten, Timens, Wim, Oliver, Brian G G, de Vries, Maaike, Brandsma, Corry-Anke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2021
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070616/
https://www.ncbi.nlm.nih.gov/pubmed/33273021
http://dx.doi.org/10.1136/thoraxjnl-2020-215114
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author Woldhuis, Roy R
Heijink, Irene H
van den Berge, Maarten
Timens, Wim
Oliver, Brian G G
de Vries, Maaike
Brandsma, Corry-Anke
author_facet Woldhuis, Roy R
Heijink, Irene H
van den Berge, Maarten
Timens, Wim
Oliver, Brian G G
de Vries, Maaike
Brandsma, Corry-Anke
author_sort Woldhuis, Roy R
collection PubMed
description COPD-derived fibroblasts have increased cellular senescence. Senescent cell accumulation can induce tissue dysfunction by their senescence-associated secretory phenotype (SASP). We aimed to determine the SASP of senescent fibroblasts and COPD-derived lung fibroblasts, including severe, early-onset (SEO)-COPD. SASP protein secretion was measured after paraquat-induced senescence in lung fibroblasts using Olink Proteomics and compared between (SEO-)COPD-derived and control-derived fibroblasts. We identified 124 SASP proteins of senescent lung fibroblasts, of which 42 were secreted at higher levels by COPD-derived fibroblasts and 35 by SEO-COPD-derived fibroblasts compared with controls. Interestingly, the (SEO-)COPD-associated SASP included proteins involved in chronic inflammation, which may contribute to (SEO-)COPD pathogenesis.
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spelling pubmed-80706162021-05-11 COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins Woldhuis, Roy R Heijink, Irene H van den Berge, Maarten Timens, Wim Oliver, Brian G G de Vries, Maaike Brandsma, Corry-Anke Thorax Brief Communication COPD-derived fibroblasts have increased cellular senescence. Senescent cell accumulation can induce tissue dysfunction by their senescence-associated secretory phenotype (SASP). We aimed to determine the SASP of senescent fibroblasts and COPD-derived lung fibroblasts, including severe, early-onset (SEO)-COPD. SASP protein secretion was measured after paraquat-induced senescence in lung fibroblasts using Olink Proteomics and compared between (SEO-)COPD-derived and control-derived fibroblasts. We identified 124 SASP proteins of senescent lung fibroblasts, of which 42 were secreted at higher levels by COPD-derived fibroblasts and 35 by SEO-COPD-derived fibroblasts compared with controls. Interestingly, the (SEO-)COPD-associated SASP included proteins involved in chronic inflammation, which may contribute to (SEO-)COPD pathogenesis. BMJ Publishing Group 2021-05 2020-12-03 /pmc/articles/PMC8070616/ /pubmed/33273021 http://dx.doi.org/10.1136/thoraxjnl-2020-215114 Text en © Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Communication
Woldhuis, Roy R
Heijink, Irene H
van den Berge, Maarten
Timens, Wim
Oliver, Brian G G
de Vries, Maaike
Brandsma, Corry-Anke
COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins
title COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins
title_full COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins
title_fullStr COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins
title_full_unstemmed COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins
title_short COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins
title_sort copd-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070616/
https://www.ncbi.nlm.nih.gov/pubmed/33273021
http://dx.doi.org/10.1136/thoraxjnl-2020-215114
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