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Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology

RNA viruses cause a wide range of human diseases that are associated with high mortality and morbidity. In the past decades, the rise of genetic-based screening methods and high-throughput sequencing approaches allowed the uncovering of unique and elusive aspects of RNA virus replication and pathoge...

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Autores principales: Simanjuntak, Yogy, Schamoni-Kast, Kira, Grün, Alice, Uetrecht, Charlotte, Scaturro, Pietro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070632/
https://www.ncbi.nlm.nih.gov/pubmed/33924391
http://dx.doi.org/10.3390/v13040668
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author Simanjuntak, Yogy
Schamoni-Kast, Kira
Grün, Alice
Uetrecht, Charlotte
Scaturro, Pietro
author_facet Simanjuntak, Yogy
Schamoni-Kast, Kira
Grün, Alice
Uetrecht, Charlotte
Scaturro, Pietro
author_sort Simanjuntak, Yogy
collection PubMed
description RNA viruses cause a wide range of human diseases that are associated with high mortality and morbidity. In the past decades, the rise of genetic-based screening methods and high-throughput sequencing approaches allowed the uncovering of unique and elusive aspects of RNA virus replication and pathogenesis at an unprecedented scale. However, viruses often hijack critical host functions or trigger pathological dysfunctions, perturbing cellular proteostasis, macromolecular complex organization or stoichiometry, and post-translational modifications. Such effects require the monitoring of proteins and proteoforms both on a global scale and at the structural level. Mass spectrometry (MS) has recently emerged as an important component of the RNA virus biology toolbox, with its potential to shed light on critical aspects of virus–host perturbations and streamline the identification of antiviral targets. Moreover, multiple novel MS tools are available to study the structure of large protein complexes, providing detailed information on the exact stoichiometry of cellular and viral protein complexes and critical mechanistic insights into their functions. Here, we review top-down and bottom-up mass spectrometry-based approaches in RNA virus biology with a special focus on the most recent developments in characterizing host responses, and their translational implications to identify novel tractable antiviral targets.
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spelling pubmed-80706322021-04-26 Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology Simanjuntak, Yogy Schamoni-Kast, Kira Grün, Alice Uetrecht, Charlotte Scaturro, Pietro Viruses Review RNA viruses cause a wide range of human diseases that are associated with high mortality and morbidity. In the past decades, the rise of genetic-based screening methods and high-throughput sequencing approaches allowed the uncovering of unique and elusive aspects of RNA virus replication and pathogenesis at an unprecedented scale. However, viruses often hijack critical host functions or trigger pathological dysfunctions, perturbing cellular proteostasis, macromolecular complex organization or stoichiometry, and post-translational modifications. Such effects require the monitoring of proteins and proteoforms both on a global scale and at the structural level. Mass spectrometry (MS) has recently emerged as an important component of the RNA virus biology toolbox, with its potential to shed light on critical aspects of virus–host perturbations and streamline the identification of antiviral targets. Moreover, multiple novel MS tools are available to study the structure of large protein complexes, providing detailed information on the exact stoichiometry of cellular and viral protein complexes and critical mechanistic insights into their functions. Here, we review top-down and bottom-up mass spectrometry-based approaches in RNA virus biology with a special focus on the most recent developments in characterizing host responses, and their translational implications to identify novel tractable antiviral targets. MDPI 2021-04-13 /pmc/articles/PMC8070632/ /pubmed/33924391 http://dx.doi.org/10.3390/v13040668 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Simanjuntak, Yogy
Schamoni-Kast, Kira
Grün, Alice
Uetrecht, Charlotte
Scaturro, Pietro
Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology
title Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology
title_full Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology
title_fullStr Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology
title_full_unstemmed Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology
title_short Top-Down and Bottom-Up Proteomics Methods to Study RNA Virus Biology
title_sort top-down and bottom-up proteomics methods to study rna virus biology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070632/
https://www.ncbi.nlm.nih.gov/pubmed/33924391
http://dx.doi.org/10.3390/v13040668
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