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Detection of SARS-COV-2 Proteins Using an ELISA Test
The coronavirus disease 2019 (COVID-19) global pandemic created an unprecedented public health emergency. Early recognition of an infected person and disruption of the transmission pathway are the keys to controlling this major public health threat around the world. The scientifically reliable scree...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070680/ https://www.ncbi.nlm.nih.gov/pubmed/33919728 http://dx.doi.org/10.3390/diagnostics11040698 |
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author | Di Domenico, Marina De Rosa, Alfredo Boccellino, Mariarosaria |
author_facet | Di Domenico, Marina De Rosa, Alfredo Boccellino, Mariarosaria |
author_sort | Di Domenico, Marina |
collection | PubMed |
description | The coronavirus disease 2019 (COVID-19) global pandemic created an unprecedented public health emergency. Early recognition of an infected person and disruption of the transmission pathway are the keys to controlling this major public health threat around the world. The scientifically reliable screening method is an RT-PCR test that is performed on an ororhinopharyngeal swab in the laboratory. In the current severe SARS-CoV-2 pandemic, it is necessary to identify devices for rapid diagnosis to reduce the spread of the disease. The aim of this study was to provide a qualitative, rapid, sensitive, and specific method for a diagnosis of SARS-CoV-2 infection based on the recognition of specific antigens of the SARS-CoV-2 virus. The device was built by assembling commercially available and custom-made semi-finished products. The method was performed in environments outside the laboratory, i.e., “patient side,” with an immediate chemocolorimetric response or with a digital reader using an ELISA method. |
format | Online Article Text |
id | pubmed-8070680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80706802021-04-26 Detection of SARS-COV-2 Proteins Using an ELISA Test Di Domenico, Marina De Rosa, Alfredo Boccellino, Mariarosaria Diagnostics (Basel) Technical Note The coronavirus disease 2019 (COVID-19) global pandemic created an unprecedented public health emergency. Early recognition of an infected person and disruption of the transmission pathway are the keys to controlling this major public health threat around the world. The scientifically reliable screening method is an RT-PCR test that is performed on an ororhinopharyngeal swab in the laboratory. In the current severe SARS-CoV-2 pandemic, it is necessary to identify devices for rapid diagnosis to reduce the spread of the disease. The aim of this study was to provide a qualitative, rapid, sensitive, and specific method for a diagnosis of SARS-CoV-2 infection based on the recognition of specific antigens of the SARS-CoV-2 virus. The device was built by assembling commercially available and custom-made semi-finished products. The method was performed in environments outside the laboratory, i.e., “patient side,” with an immediate chemocolorimetric response or with a digital reader using an ELISA method. MDPI 2021-04-14 /pmc/articles/PMC8070680/ /pubmed/33919728 http://dx.doi.org/10.3390/diagnostics11040698 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Technical Note Di Domenico, Marina De Rosa, Alfredo Boccellino, Mariarosaria Detection of SARS-COV-2 Proteins Using an ELISA Test |
title | Detection of SARS-COV-2 Proteins Using an ELISA Test |
title_full | Detection of SARS-COV-2 Proteins Using an ELISA Test |
title_fullStr | Detection of SARS-COV-2 Proteins Using an ELISA Test |
title_full_unstemmed | Detection of SARS-COV-2 Proteins Using an ELISA Test |
title_short | Detection of SARS-COV-2 Proteins Using an ELISA Test |
title_sort | detection of sars-cov-2 proteins using an elisa test |
topic | Technical Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070680/ https://www.ncbi.nlm.nih.gov/pubmed/33919728 http://dx.doi.org/10.3390/diagnostics11040698 |
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