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Presence of Broad-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Zoo Mammals

Broad-spectrum beta-lactamase (BSBL)-producing Enterobacteriaceae impose public health threats. With increased popularity of zoos, exotic animals are brought in close proximity of humans, making them important BSBL reservoirs. However, not much is known on the presence of BSBLs in zoos in Western Eu...

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Autores principales: De Witte, Chloë, Vereecke, Nick, Theuns, Sebastiaan, De Ruyck, Claudia, Vercammen, Francis, Bouts, Tim, Boyen, Filip, Nauwynck, Hans, Haesebrouck, Freddy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070755/
https://www.ncbi.nlm.nih.gov/pubmed/33919869
http://dx.doi.org/10.3390/microorganisms9040834
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author De Witte, Chloë
Vereecke, Nick
Theuns, Sebastiaan
De Ruyck, Claudia
Vercammen, Francis
Bouts, Tim
Boyen, Filip
Nauwynck, Hans
Haesebrouck, Freddy
author_facet De Witte, Chloë
Vereecke, Nick
Theuns, Sebastiaan
De Ruyck, Claudia
Vercammen, Francis
Bouts, Tim
Boyen, Filip
Nauwynck, Hans
Haesebrouck, Freddy
author_sort De Witte, Chloë
collection PubMed
description Broad-spectrum beta-lactamase (BSBL)-producing Enterobacteriaceae impose public health threats. With increased popularity of zoos, exotic animals are brought in close proximity of humans, making them important BSBL reservoirs. However, not much is known on the presence of BSBLs in zoos in Western Europe. Fecal carriage of BSBL-producing Enterobacteriaceae was investigated in 38 zoo mammals from two Belgian zoos. Presence of bla-genes was investigated using PCR, followed by whole-genome sequencing and Fourier-transform infrared spectroscopy to cluster acquired resistance encoding genes and clonality of BSBL-producing isolates. Thirty-five putatively ceftiofur-resistant isolates were obtained from 52.6% of the zoo mammals. Most isolates were identified as E. coli (25/35), of which 64.0% showed multidrug resistance (MDR). Most frequently detected bla-genes were CTX-M-1 (17/25) and TEM-1 (4/25). Phylogenetic trees confirmed clustering of almost all E. coli isolates obtained from the same animal species. Clustering of five isolates from an Amur tiger, an Amur leopard, and a spectacled bear was observed in Zoo 1, as well as for five isolates from a spotted hyena and an African lion in Zoo 2. This might indicate clonal expansion of an E. coli strain in both zoos. In conclusion, MDR BSBL-producing bacteria were shown to be present in the fecal microbiota of zoo mammals in two zoos in Belgium. Further research is necessary to investigate if these bacteria pose zoonotic and health risks.
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spelling pubmed-80707552021-04-26 Presence of Broad-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Zoo Mammals De Witte, Chloë Vereecke, Nick Theuns, Sebastiaan De Ruyck, Claudia Vercammen, Francis Bouts, Tim Boyen, Filip Nauwynck, Hans Haesebrouck, Freddy Microorganisms Article Broad-spectrum beta-lactamase (BSBL)-producing Enterobacteriaceae impose public health threats. With increased popularity of zoos, exotic animals are brought in close proximity of humans, making them important BSBL reservoirs. However, not much is known on the presence of BSBLs in zoos in Western Europe. Fecal carriage of BSBL-producing Enterobacteriaceae was investigated in 38 zoo mammals from two Belgian zoos. Presence of bla-genes was investigated using PCR, followed by whole-genome sequencing and Fourier-transform infrared spectroscopy to cluster acquired resistance encoding genes and clonality of BSBL-producing isolates. Thirty-five putatively ceftiofur-resistant isolates were obtained from 52.6% of the zoo mammals. Most isolates were identified as E. coli (25/35), of which 64.0% showed multidrug resistance (MDR). Most frequently detected bla-genes were CTX-M-1 (17/25) and TEM-1 (4/25). Phylogenetic trees confirmed clustering of almost all E. coli isolates obtained from the same animal species. Clustering of five isolates from an Amur tiger, an Amur leopard, and a spectacled bear was observed in Zoo 1, as well as for five isolates from a spotted hyena and an African lion in Zoo 2. This might indicate clonal expansion of an E. coli strain in both zoos. In conclusion, MDR BSBL-producing bacteria were shown to be present in the fecal microbiota of zoo mammals in two zoos in Belgium. Further research is necessary to investigate if these bacteria pose zoonotic and health risks. MDPI 2021-04-14 /pmc/articles/PMC8070755/ /pubmed/33919869 http://dx.doi.org/10.3390/microorganisms9040834 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Witte, Chloë
Vereecke, Nick
Theuns, Sebastiaan
De Ruyck, Claudia
Vercammen, Francis
Bouts, Tim
Boyen, Filip
Nauwynck, Hans
Haesebrouck, Freddy
Presence of Broad-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Zoo Mammals
title Presence of Broad-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Zoo Mammals
title_full Presence of Broad-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Zoo Mammals
title_fullStr Presence of Broad-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Zoo Mammals
title_full_unstemmed Presence of Broad-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Zoo Mammals
title_short Presence of Broad-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Zoo Mammals
title_sort presence of broad-spectrum beta-lactamase-producing enterobacteriaceae in zoo mammals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070755/
https://www.ncbi.nlm.nih.gov/pubmed/33919869
http://dx.doi.org/10.3390/microorganisms9040834
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