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Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods

The main aim of this work was to verify the metabolic and functional aptitude of 15 vaginal strains belonging to Lactobacillus crispatus, Lactobacillus gasseri, and Limosilactobacillus vaginalis (previously Lactobacillus vaginalis), already characterized for their technological and antimicrobial pro...

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Autores principales: D’Alessandro, Margherita, Parolin, Carola, Bukvicki, Danka, Siroli, Lorenzo, Vitali, Beatrice, De Angelis, Maria, Lanciotti, Rosalba, Patrignani, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070825/
https://www.ncbi.nlm.nih.gov/pubmed/33919838
http://dx.doi.org/10.3390/microorganisms9040833
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author D’Alessandro, Margherita
Parolin, Carola
Bukvicki, Danka
Siroli, Lorenzo
Vitali, Beatrice
De Angelis, Maria
Lanciotti, Rosalba
Patrignani, Francesca
author_facet D’Alessandro, Margherita
Parolin, Carola
Bukvicki, Danka
Siroli, Lorenzo
Vitali, Beatrice
De Angelis, Maria
Lanciotti, Rosalba
Patrignani, Francesca
author_sort D’Alessandro, Margherita
collection PubMed
description The main aim of this work was to verify the metabolic and functional aptitude of 15 vaginal strains belonging to Lactobacillus crispatus, Lactobacillus gasseri, and Limosilactobacillus vaginalis (previously Lactobacillus vaginalis), already characterized for their technological and antimicrobial properties. In order to evaluate the metabolic profile of these vaginal strains, a phenotype microarray analysis was performed on them. Functional parameters such as hydrophobicity, auto-aggregation, deconjugation of bile salts, adhesion to an intestinal cell line (Caco-2), and a simulated digestion process were evaluated for these strains. A good number of these strains showed hydrophobicity values higher than 70%. Regarding the auto-aggregation assay, the most promising strains were L. crispatus BC9 and BC1, L. gasseri BC10 and BC14, and L. vaginalis BC17. Moreover, L. crispatus BC4, BC6, BC7, and BC8 were characterized by strong bile salts hydrolase activity (BHS). In addition, L. crispatus BC8 and L. vaginalis BC17 were characterized by a medium ability to adhere to Caco-2 cells. Data related to digestion process showed a strong ability of vaginal lactobacilli to withstand this stress. In conclusion, the data collected show the metabolic versatility and several exploitable functional properties of the investigated vaginal lactobacilli.
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spelling pubmed-80708252021-04-26 Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods D’Alessandro, Margherita Parolin, Carola Bukvicki, Danka Siroli, Lorenzo Vitali, Beatrice De Angelis, Maria Lanciotti, Rosalba Patrignani, Francesca Microorganisms Article The main aim of this work was to verify the metabolic and functional aptitude of 15 vaginal strains belonging to Lactobacillus crispatus, Lactobacillus gasseri, and Limosilactobacillus vaginalis (previously Lactobacillus vaginalis), already characterized for their technological and antimicrobial properties. In order to evaluate the metabolic profile of these vaginal strains, a phenotype microarray analysis was performed on them. Functional parameters such as hydrophobicity, auto-aggregation, deconjugation of bile salts, adhesion to an intestinal cell line (Caco-2), and a simulated digestion process were evaluated for these strains. A good number of these strains showed hydrophobicity values higher than 70%. Regarding the auto-aggregation assay, the most promising strains were L. crispatus BC9 and BC1, L. gasseri BC10 and BC14, and L. vaginalis BC17. Moreover, L. crispatus BC4, BC6, BC7, and BC8 were characterized by strong bile salts hydrolase activity (BHS). In addition, L. crispatus BC8 and L. vaginalis BC17 were characterized by a medium ability to adhere to Caco-2 cells. Data related to digestion process showed a strong ability of vaginal lactobacilli to withstand this stress. In conclusion, the data collected show the metabolic versatility and several exploitable functional properties of the investigated vaginal lactobacilli. MDPI 2021-04-14 /pmc/articles/PMC8070825/ /pubmed/33919838 http://dx.doi.org/10.3390/microorganisms9040833 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Alessandro, Margherita
Parolin, Carola
Bukvicki, Danka
Siroli, Lorenzo
Vitali, Beatrice
De Angelis, Maria
Lanciotti, Rosalba
Patrignani, Francesca
Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods
title Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods
title_full Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods
title_fullStr Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods
title_full_unstemmed Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods
title_short Probiotic and Metabolic Characterization of Vaginal Lactobacilli for a Potential Use in Functional Foods
title_sort probiotic and metabolic characterization of vaginal lactobacilli for a potential use in functional foods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070825/
https://www.ncbi.nlm.nih.gov/pubmed/33919838
http://dx.doi.org/10.3390/microorganisms9040833
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