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Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions
Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070910/ https://www.ncbi.nlm.nih.gov/pubmed/33919977 http://dx.doi.org/10.3390/ijms22084053 |
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author | Bączyńska, Ewa Pels, Katarzyna Karolina Basu, Subhadip Włodarczyk, Jakub Ruszczycki, Błażej |
author_facet | Bączyńska, Ewa Pels, Katarzyna Karolina Basu, Subhadip Włodarczyk, Jakub Ruszczycki, Błażej |
author_sort | Bączyńska, Ewa |
collection | PubMed |
description | Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images is one of the key elements towards understanding mechanisms of structural neuronal plasticity and bases of brain pathology. In the following article, we review experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. We compare various methodological pipelines of biological models, sample preparation, data analysis, image acquisition, sample size, and statistical analysis. The methodology and results of relevant experiments are systematically summarized in a tabular form. In particular, we focus on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance. |
format | Online Article Text |
id | pubmed-8070910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80709102021-04-26 Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions Bączyńska, Ewa Pels, Katarzyna Karolina Basu, Subhadip Włodarczyk, Jakub Ruszczycki, Błażej Int J Mol Sci Review Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images is one of the key elements towards understanding mechanisms of structural neuronal plasticity and bases of brain pathology. In the following article, we review experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. We compare various methodological pipelines of biological models, sample preparation, data analysis, image acquisition, sample size, and statistical analysis. The methodology and results of relevant experiments are systematically summarized in a tabular form. In particular, we focus on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance. MDPI 2021-04-14 /pmc/articles/PMC8070910/ /pubmed/33919977 http://dx.doi.org/10.3390/ijms22084053 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Bączyńska, Ewa Pels, Katarzyna Karolina Basu, Subhadip Włodarczyk, Jakub Ruszczycki, Błażej Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
title | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
title_full | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
title_fullStr | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
title_full_unstemmed | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
title_short | Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions |
title_sort | quantification of dendritic spines remodeling under physiological stimuli and in pathological conditions |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070910/ https://www.ncbi.nlm.nih.gov/pubmed/33919977 http://dx.doi.org/10.3390/ijms22084053 |
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