Cargando…
Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus
Influenza A viruses (IAV) of subtype H9N2, endemic in world-wide poultry holdings, are reported to cause spill-over infections to pigs and humans and have also contributed substantially to recent reassortment-derived pre-pandemic zoonotic viruses of concern, such as the Asian H7N9 viruses. Recently,...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070959/ https://www.ncbi.nlm.nih.gov/pubmed/33919890 http://dx.doi.org/10.3390/v13040672 |
_version_ | 1783683589735448576 |
---|---|
author | Halwe, Nico Joel Gorka, Marco Hoffmann, Bernd Rissmann, Melanie Breithaupt, Angele Schwemmle, Martin Beer, Martin Kandeil, Ahmed Ali, Mohamed A. Kayali, Ghazi Hoffmann, Donata Balkema-Buschmann, Anne |
author_facet | Halwe, Nico Joel Gorka, Marco Hoffmann, Bernd Rissmann, Melanie Breithaupt, Angele Schwemmle, Martin Beer, Martin Kandeil, Ahmed Ali, Mohamed A. Kayali, Ghazi Hoffmann, Donata Balkema-Buschmann, Anne |
author_sort | Halwe, Nico Joel |
collection | PubMed |
description | Influenza A viruses (IAV) of subtype H9N2, endemic in world-wide poultry holdings, are reported to cause spill-over infections to pigs and humans and have also contributed substantially to recent reassortment-derived pre-pandemic zoonotic viruses of concern, such as the Asian H7N9 viruses. Recently, a H9N2 bat influenza A virus was found in Egyptian fruit bats (Rousettus aegyptiacus), raising the question of whether this bat species is a suitable host for IAV. Here, we studied the susceptibility, pathogenesis and transmission of avian and bat-related H9N2 viruses in this new host. In a first experiment, we oronasally inoculated six Egyptian fruit bats with an avian-related H9N2 virus (A/layer chicken/Bangladesh/VP02-plaque/2016 (H9N2)). In a second experiment, six Egyptian fruit bats were inoculated with the newly discovered bat-related H9N2 virus (A/bat/Egypt/381OP/2017 (H9N2)). While R. aegyptiacus turned out to be refractory to an infection with H9N2 avian-type, inoculation with the bat H9N2 subtype established a productive infection in all inoculated animals with a detectable seroconversion at day 21 post-infection. In conclusion, Egyptian fruit bats are most likely not susceptible to the avian H9N2 subtype, but can be infected with fruit bat-derived H9N2. H9-specific sero-reactivities in fruit bats in the field are therefore more likely the result of contact with a bat-adapted H9N2 strain. |
format | Online Article Text |
id | pubmed-8070959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80709592021-04-26 Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus Halwe, Nico Joel Gorka, Marco Hoffmann, Bernd Rissmann, Melanie Breithaupt, Angele Schwemmle, Martin Beer, Martin Kandeil, Ahmed Ali, Mohamed A. Kayali, Ghazi Hoffmann, Donata Balkema-Buschmann, Anne Viruses Article Influenza A viruses (IAV) of subtype H9N2, endemic in world-wide poultry holdings, are reported to cause spill-over infections to pigs and humans and have also contributed substantially to recent reassortment-derived pre-pandemic zoonotic viruses of concern, such as the Asian H7N9 viruses. Recently, a H9N2 bat influenza A virus was found in Egyptian fruit bats (Rousettus aegyptiacus), raising the question of whether this bat species is a suitable host for IAV. Here, we studied the susceptibility, pathogenesis and transmission of avian and bat-related H9N2 viruses in this new host. In a first experiment, we oronasally inoculated six Egyptian fruit bats with an avian-related H9N2 virus (A/layer chicken/Bangladesh/VP02-plaque/2016 (H9N2)). In a second experiment, six Egyptian fruit bats were inoculated with the newly discovered bat-related H9N2 virus (A/bat/Egypt/381OP/2017 (H9N2)). While R. aegyptiacus turned out to be refractory to an infection with H9N2 avian-type, inoculation with the bat H9N2 subtype established a productive infection in all inoculated animals with a detectable seroconversion at day 21 post-infection. In conclusion, Egyptian fruit bats are most likely not susceptible to the avian H9N2 subtype, but can be infected with fruit bat-derived H9N2. H9-specific sero-reactivities in fruit bats in the field are therefore more likely the result of contact with a bat-adapted H9N2 strain. MDPI 2021-04-14 /pmc/articles/PMC8070959/ /pubmed/33919890 http://dx.doi.org/10.3390/v13040672 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Halwe, Nico Joel Gorka, Marco Hoffmann, Bernd Rissmann, Melanie Breithaupt, Angele Schwemmle, Martin Beer, Martin Kandeil, Ahmed Ali, Mohamed A. Kayali, Ghazi Hoffmann, Donata Balkema-Buschmann, Anne Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus |
title | Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus |
title_full | Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus |
title_fullStr | Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus |
title_full_unstemmed | Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus |
title_short | Egyptian Fruit Bats (Rousettus aegyptiacus) Were Resistant to Experimental Inoculation with Avian-Origin Influenza A Virus of Subtype H9N2, But Are Susceptible to Experimental Infection with Bat-Borne H9N2 Virus |
title_sort | egyptian fruit bats (rousettus aegyptiacus) were resistant to experimental inoculation with avian-origin influenza a virus of subtype h9n2, but are susceptible to experimental infection with bat-borne h9n2 virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070959/ https://www.ncbi.nlm.nih.gov/pubmed/33919890 http://dx.doi.org/10.3390/v13040672 |
work_keys_str_mv | AT halwenicojoel egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT gorkamarco egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT hoffmannbernd egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT rissmannmelanie egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT breithauptangele egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT schwemmlemartin egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT beermartin egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT kandeilahmed egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT alimohameda egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT kayalighazi egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT hoffmanndonata egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus AT balkemabuschmannanne egyptianfruitbatsrousettusaegyptiacuswereresistanttoexperimentalinoculationwithavianorigininfluenzaavirusofsubtypeh9n2butaresusceptibletoexperimentalinfectionwithbatborneh9n2virus |