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Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal
The diversity of aerobic anoxygenic phototrophs (AAPs) and rhodopsin-containing bacteria in the surface microlayer, water column, and epilithic biofilms of Lake Baikal was studied for the first time, employing pufM and rhodopsin genes, and compared to 16S rRNA diversity. We detected pufM-containing...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071047/ https://www.ncbi.nlm.nih.gov/pubmed/33920057 http://dx.doi.org/10.3390/microorganisms9040842 |
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author | Galachyants, Agnia Dmitrievna Krasnopeev, Andrey Yurjevich Podlesnaya, Galina Vladimirovna Potapov, Sergey Anatoljevich Sukhanova, Elena Viktorovna Tikhonova, Irina Vasiljevna Zimens, Ekaterina Andreevna Kabilov, Marsel Rasimovich Zhuchenko, Natalia Albertovna Gorshkova, Anna Sergeevna Suslova, Maria Yurjevna Belykh, Olga Ivanovna |
author_facet | Galachyants, Agnia Dmitrievna Krasnopeev, Andrey Yurjevich Podlesnaya, Galina Vladimirovna Potapov, Sergey Anatoljevich Sukhanova, Elena Viktorovna Tikhonova, Irina Vasiljevna Zimens, Ekaterina Andreevna Kabilov, Marsel Rasimovich Zhuchenko, Natalia Albertovna Gorshkova, Anna Sergeevna Suslova, Maria Yurjevna Belykh, Olga Ivanovna |
author_sort | Galachyants, Agnia Dmitrievna |
collection | PubMed |
description | The diversity of aerobic anoxygenic phototrophs (AAPs) and rhodopsin-containing bacteria in the surface microlayer, water column, and epilithic biofilms of Lake Baikal was studied for the first time, employing pufM and rhodopsin genes, and compared to 16S rRNA diversity. We detected pufM-containing Alphaproteobacteria (orders Rhodobacterales, Rhizobiales, Rhodospirillales, and Sphingomonadales), Betaproteobacteria (order Burkholderiales), Gemmatimonadetes, and Planctomycetes. Rhodobacterales dominated all the studied biotopes. The diversity of rhodopsin-containing bacteria in neuston and plankton of Lake Baikal was comparable to other studied water bodies. Bacteroidetes along with Proteobacteria were the prevailing phyla, and Verrucomicrobia and Planctomycetes were also detected. The number of rhodopsin sequences unclassified to the phylum level was rather high: 29% in the water microbiomes and 22% in the epilithon. Diversity of rhodopsin-containing bacteria in epilithic biofilms was comparable with that in neuston and plankton at the phyla level. Unweighted pair group method with arithmetic mean (UPGMA) and non-metric multidimensional scaling (NMDS) analysis indicated a distinct discrepancy between epilithon and microbial communities of water (including neuston and plankton) in the 16S rRNA, pufM and rhodopsin genes. |
format | Online Article Text |
id | pubmed-8071047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80710472021-04-26 Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal Galachyants, Agnia Dmitrievna Krasnopeev, Andrey Yurjevich Podlesnaya, Galina Vladimirovna Potapov, Sergey Anatoljevich Sukhanova, Elena Viktorovna Tikhonova, Irina Vasiljevna Zimens, Ekaterina Andreevna Kabilov, Marsel Rasimovich Zhuchenko, Natalia Albertovna Gorshkova, Anna Sergeevna Suslova, Maria Yurjevna Belykh, Olga Ivanovna Microorganisms Article The diversity of aerobic anoxygenic phototrophs (AAPs) and rhodopsin-containing bacteria in the surface microlayer, water column, and epilithic biofilms of Lake Baikal was studied for the first time, employing pufM and rhodopsin genes, and compared to 16S rRNA diversity. We detected pufM-containing Alphaproteobacteria (orders Rhodobacterales, Rhizobiales, Rhodospirillales, and Sphingomonadales), Betaproteobacteria (order Burkholderiales), Gemmatimonadetes, and Planctomycetes. Rhodobacterales dominated all the studied biotopes. The diversity of rhodopsin-containing bacteria in neuston and plankton of Lake Baikal was comparable to other studied water bodies. Bacteroidetes along with Proteobacteria were the prevailing phyla, and Verrucomicrobia and Planctomycetes were also detected. The number of rhodopsin sequences unclassified to the phylum level was rather high: 29% in the water microbiomes and 22% in the epilithon. Diversity of rhodopsin-containing bacteria in epilithic biofilms was comparable with that in neuston and plankton at the phyla level. Unweighted pair group method with arithmetic mean (UPGMA) and non-metric multidimensional scaling (NMDS) analysis indicated a distinct discrepancy between epilithon and microbial communities of water (including neuston and plankton) in the 16S rRNA, pufM and rhodopsin genes. MDPI 2021-04-14 /pmc/articles/PMC8071047/ /pubmed/33920057 http://dx.doi.org/10.3390/microorganisms9040842 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Galachyants, Agnia Dmitrievna Krasnopeev, Andrey Yurjevich Podlesnaya, Galina Vladimirovna Potapov, Sergey Anatoljevich Sukhanova, Elena Viktorovna Tikhonova, Irina Vasiljevna Zimens, Ekaterina Andreevna Kabilov, Marsel Rasimovich Zhuchenko, Natalia Albertovna Gorshkova, Anna Sergeevna Suslova, Maria Yurjevna Belykh, Olga Ivanovna Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal |
title | Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal |
title_full | Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal |
title_fullStr | Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal |
title_full_unstemmed | Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal |
title_short | Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal |
title_sort | diversity of aerobic anoxygenic phototrophs and rhodopsin-containing bacteria in the surface microlayer, water column and epilithic biofilms of lake baikal |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071047/ https://www.ncbi.nlm.nih.gov/pubmed/33920057 http://dx.doi.org/10.3390/microorganisms9040842 |
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