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Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal

The diversity of aerobic anoxygenic phototrophs (AAPs) and rhodopsin-containing bacteria in the surface microlayer, water column, and epilithic biofilms of Lake Baikal was studied for the first time, employing pufM and rhodopsin genes, and compared to 16S rRNA diversity. We detected pufM-containing...

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Autores principales: Galachyants, Agnia Dmitrievna, Krasnopeev, Andrey Yurjevich, Podlesnaya, Galina Vladimirovna, Potapov, Sergey Anatoljevich, Sukhanova, Elena Viktorovna, Tikhonova, Irina Vasiljevna, Zimens, Ekaterina Andreevna, Kabilov, Marsel Rasimovich, Zhuchenko, Natalia Albertovna, Gorshkova, Anna Sergeevna, Suslova, Maria Yurjevna, Belykh, Olga Ivanovna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071047/
https://www.ncbi.nlm.nih.gov/pubmed/33920057
http://dx.doi.org/10.3390/microorganisms9040842
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author Galachyants, Agnia Dmitrievna
Krasnopeev, Andrey Yurjevich
Podlesnaya, Galina Vladimirovna
Potapov, Sergey Anatoljevich
Sukhanova, Elena Viktorovna
Tikhonova, Irina Vasiljevna
Zimens, Ekaterina Andreevna
Kabilov, Marsel Rasimovich
Zhuchenko, Natalia Albertovna
Gorshkova, Anna Sergeevna
Suslova, Maria Yurjevna
Belykh, Olga Ivanovna
author_facet Galachyants, Agnia Dmitrievna
Krasnopeev, Andrey Yurjevich
Podlesnaya, Galina Vladimirovna
Potapov, Sergey Anatoljevich
Sukhanova, Elena Viktorovna
Tikhonova, Irina Vasiljevna
Zimens, Ekaterina Andreevna
Kabilov, Marsel Rasimovich
Zhuchenko, Natalia Albertovna
Gorshkova, Anna Sergeevna
Suslova, Maria Yurjevna
Belykh, Olga Ivanovna
author_sort Galachyants, Agnia Dmitrievna
collection PubMed
description The diversity of aerobic anoxygenic phototrophs (AAPs) and rhodopsin-containing bacteria in the surface microlayer, water column, and epilithic biofilms of Lake Baikal was studied for the first time, employing pufM and rhodopsin genes, and compared to 16S rRNA diversity. We detected pufM-containing Alphaproteobacteria (orders Rhodobacterales, Rhizobiales, Rhodospirillales, and Sphingomonadales), Betaproteobacteria (order Burkholderiales), Gemmatimonadetes, and Planctomycetes. Rhodobacterales dominated all the studied biotopes. The diversity of rhodopsin-containing bacteria in neuston and plankton of Lake Baikal was comparable to other studied water bodies. Bacteroidetes along with Proteobacteria were the prevailing phyla, and Verrucomicrobia and Planctomycetes were also detected. The number of rhodopsin sequences unclassified to the phylum level was rather high: 29% in the water microbiomes and 22% in the epilithon. Diversity of rhodopsin-containing bacteria in epilithic biofilms was comparable with that in neuston and plankton at the phyla level. Unweighted pair group method with arithmetic mean (UPGMA) and non-metric multidimensional scaling (NMDS) analysis indicated a distinct discrepancy between epilithon and microbial communities of water (including neuston and plankton) in the 16S rRNA, pufM and rhodopsin genes.
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spelling pubmed-80710472021-04-26 Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal Galachyants, Agnia Dmitrievna Krasnopeev, Andrey Yurjevich Podlesnaya, Galina Vladimirovna Potapov, Sergey Anatoljevich Sukhanova, Elena Viktorovna Tikhonova, Irina Vasiljevna Zimens, Ekaterina Andreevna Kabilov, Marsel Rasimovich Zhuchenko, Natalia Albertovna Gorshkova, Anna Sergeevna Suslova, Maria Yurjevna Belykh, Olga Ivanovna Microorganisms Article The diversity of aerobic anoxygenic phototrophs (AAPs) and rhodopsin-containing bacteria in the surface microlayer, water column, and epilithic biofilms of Lake Baikal was studied for the first time, employing pufM and rhodopsin genes, and compared to 16S rRNA diversity. We detected pufM-containing Alphaproteobacteria (orders Rhodobacterales, Rhizobiales, Rhodospirillales, and Sphingomonadales), Betaproteobacteria (order Burkholderiales), Gemmatimonadetes, and Planctomycetes. Rhodobacterales dominated all the studied biotopes. The diversity of rhodopsin-containing bacteria in neuston and plankton of Lake Baikal was comparable to other studied water bodies. Bacteroidetes along with Proteobacteria were the prevailing phyla, and Verrucomicrobia and Planctomycetes were also detected. The number of rhodopsin sequences unclassified to the phylum level was rather high: 29% in the water microbiomes and 22% in the epilithon. Diversity of rhodopsin-containing bacteria in epilithic biofilms was comparable with that in neuston and plankton at the phyla level. Unweighted pair group method with arithmetic mean (UPGMA) and non-metric multidimensional scaling (NMDS) analysis indicated a distinct discrepancy between epilithon and microbial communities of water (including neuston and plankton) in the 16S rRNA, pufM and rhodopsin genes. MDPI 2021-04-14 /pmc/articles/PMC8071047/ /pubmed/33920057 http://dx.doi.org/10.3390/microorganisms9040842 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Galachyants, Agnia Dmitrievna
Krasnopeev, Andrey Yurjevich
Podlesnaya, Galina Vladimirovna
Potapov, Sergey Anatoljevich
Sukhanova, Elena Viktorovna
Tikhonova, Irina Vasiljevna
Zimens, Ekaterina Andreevna
Kabilov, Marsel Rasimovich
Zhuchenko, Natalia Albertovna
Gorshkova, Anna Sergeevna
Suslova, Maria Yurjevna
Belykh, Olga Ivanovna
Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal
title Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal
title_full Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal
title_fullStr Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal
title_full_unstemmed Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal
title_short Diversity of Aerobic Anoxygenic Phototrophs and Rhodopsin-Containing Bacteria in the Surface Microlayer, Water Column and Epilithic Biofilms of Lake Baikal
title_sort diversity of aerobic anoxygenic phototrophs and rhodopsin-containing bacteria in the surface microlayer, water column and epilithic biofilms of lake baikal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071047/
https://www.ncbi.nlm.nih.gov/pubmed/33920057
http://dx.doi.org/10.3390/microorganisms9040842
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