Cargando…
Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells
(1) Background: Cynara cardunculus L. subsp. scolymus (L.) Hegi, popularly known as artichoke, is an herbaceous plant belonging to the Asteraceae family. Artichoke leaf extracts (ALEs) have been widely used in traditional medicine because of their hepatoprotective, cholagogic, hypoglycaemic, hypolip...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071198/ https://www.ncbi.nlm.nih.gov/pubmed/33920761 http://dx.doi.org/10.3390/ijerph18084166 |
_version_ | 1783683644753182720 |
---|---|
author | Villarini, Milena Acito, Mattia di Vito, Raffaella Vannini, Samuele Dominici, Luca Fatigoni, Cristina Pagiotti, Rita Moretti, Massimo |
author_facet | Villarini, Milena Acito, Mattia di Vito, Raffaella Vannini, Samuele Dominici, Luca Fatigoni, Cristina Pagiotti, Rita Moretti, Massimo |
author_sort | Villarini, Milena |
collection | PubMed |
description | (1) Background: Cynara cardunculus L. subsp. scolymus (L.) Hegi, popularly known as artichoke, is an herbaceous plant belonging to the Asteraceae family. Artichoke leaf extracts (ALEs) have been widely used in traditional medicine because of their hepatoprotective, cholagogic, hypoglycaemic, hypolipemic and antibacterial properties. ALEs are also recognized for their antioxidative and anti-inflammatory activities. In this study, we evaluated the cytotoxic, genotoxic, and apoptotic activities, as well as effect on cell growth of ALEs on human colon cancer HT-29 and RKO cells. HT-29 and RKO cells exhibit a different p53 status: RKO cells express the wild-type protein, whereas HT-29 cells express a p53-R273H contact mutant. (2) Methods: Four different ALEs were obtained by sequential extraction of dried artichoke leaves; ALEs were characterized for their content in chlorogenic acid, cynaropicrin, and caffeoylquinic acids. HT-29 and RKO cells were used for in vitro testing (i.e., cytotoxicity and genotoxicity assessment, cell cycle analysis, apoptosis induction). (3) Results: Two out of the four tested ALEs showed marked effects on cell vitality toward HT-29 and RKO tumour cells. The effect was accompanied by a genotoxic activity exerted at a non-cytotoxic concentrations, by a significant perturbation of cell cycle (i.e., with increase of cells in the sub-G(1) phase), and by the induction of apoptosis. (4) Conclusions: ALEs rich in cynaropicrin, caffeoylquinic acids, and chlorogenic acid showed to be capable of affecting HT-29 and RKO colon cancer cells by inducing favourable biological effects: cell cycle perturbation, activation of mitochondrial dependent pathway of apoptosis, and the induction of genotoxic effects probably mediated by the induction of apoptosis. Taken together, these results weigh in favour of a potential cancer chemotherapeutic activity of ALEs. |
format | Online Article Text |
id | pubmed-8071198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80711982021-04-26 Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells Villarini, Milena Acito, Mattia di Vito, Raffaella Vannini, Samuele Dominici, Luca Fatigoni, Cristina Pagiotti, Rita Moretti, Massimo Int J Environ Res Public Health Article (1) Background: Cynara cardunculus L. subsp. scolymus (L.) Hegi, popularly known as artichoke, is an herbaceous plant belonging to the Asteraceae family. Artichoke leaf extracts (ALEs) have been widely used in traditional medicine because of their hepatoprotective, cholagogic, hypoglycaemic, hypolipemic and antibacterial properties. ALEs are also recognized for their antioxidative and anti-inflammatory activities. In this study, we evaluated the cytotoxic, genotoxic, and apoptotic activities, as well as effect on cell growth of ALEs on human colon cancer HT-29 and RKO cells. HT-29 and RKO cells exhibit a different p53 status: RKO cells express the wild-type protein, whereas HT-29 cells express a p53-R273H contact mutant. (2) Methods: Four different ALEs were obtained by sequential extraction of dried artichoke leaves; ALEs were characterized for their content in chlorogenic acid, cynaropicrin, and caffeoylquinic acids. HT-29 and RKO cells were used for in vitro testing (i.e., cytotoxicity and genotoxicity assessment, cell cycle analysis, apoptosis induction). (3) Results: Two out of the four tested ALEs showed marked effects on cell vitality toward HT-29 and RKO tumour cells. The effect was accompanied by a genotoxic activity exerted at a non-cytotoxic concentrations, by a significant perturbation of cell cycle (i.e., with increase of cells in the sub-G(1) phase), and by the induction of apoptosis. (4) Conclusions: ALEs rich in cynaropicrin, caffeoylquinic acids, and chlorogenic acid showed to be capable of affecting HT-29 and RKO colon cancer cells by inducing favourable biological effects: cell cycle perturbation, activation of mitochondrial dependent pathway of apoptosis, and the induction of genotoxic effects probably mediated by the induction of apoptosis. Taken together, these results weigh in favour of a potential cancer chemotherapeutic activity of ALEs. MDPI 2021-04-15 /pmc/articles/PMC8071198/ /pubmed/33920761 http://dx.doi.org/10.3390/ijerph18084166 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Villarini, Milena Acito, Mattia di Vito, Raffaella Vannini, Samuele Dominici, Luca Fatigoni, Cristina Pagiotti, Rita Moretti, Massimo Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells |
title | Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells |
title_full | Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells |
title_fullStr | Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells |
title_full_unstemmed | Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells |
title_short | Pro-Apoptotic Activity of Artichoke Leaf Extracts in Human HT-29 and RKO Colon Cancer Cells |
title_sort | pro-apoptotic activity of artichoke leaf extracts in human ht-29 and rko colon cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071198/ https://www.ncbi.nlm.nih.gov/pubmed/33920761 http://dx.doi.org/10.3390/ijerph18084166 |
work_keys_str_mv | AT villarinimilena proapoptoticactivityofartichokeleafextractsinhumanht29andrkocoloncancercells AT acitomattia proapoptoticactivityofartichokeleafextractsinhumanht29andrkocoloncancercells AT divitoraffaella proapoptoticactivityofartichokeleafextractsinhumanht29andrkocoloncancercells AT vanninisamuele proapoptoticactivityofartichokeleafextractsinhumanht29andrkocoloncancercells AT dominiciluca proapoptoticactivityofartichokeleafextractsinhumanht29andrkocoloncancercells AT fatigonicristina proapoptoticactivityofartichokeleafextractsinhumanht29andrkocoloncancercells AT pagiottirita proapoptoticactivityofartichokeleafextractsinhumanht29andrkocoloncancercells AT morettimassimo proapoptoticactivityofartichokeleafextractsinhumanht29andrkocoloncancercells |