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Microfluidic Device for the Identification of Biological Sex by Analysis of Latent Fingermark Deposits

To date, most research regarding amino acid detection and quantification in fingermarks relies on spectrometric methods. Herein, the Sakaguchi colorimetric test was adapted to a rotationally-driven microfluidic platform and used to detect and quantify arginine in fingermarks deposited by male and fe...

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Autores principales: Marshall, Jamila S., Sita, Madelyn L., Landers, James P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071498/
https://www.ncbi.nlm.nih.gov/pubmed/33921163
http://dx.doi.org/10.3390/mi12040442
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author Marshall, Jamila S.
Sita, Madelyn L.
Landers, James P.
author_facet Marshall, Jamila S.
Sita, Madelyn L.
Landers, James P.
author_sort Marshall, Jamila S.
collection PubMed
description To date, most research regarding amino acid detection and quantification in fingermarks relies on spectrometric methods. Herein, the Sakaguchi colorimetric test was adapted to a rotationally-driven microfluidic platform and used to detect and quantify arginine in fingermarks deposited by male and female donors. A red color indicates the presence of arginine in a given sample following the reaction, and the intensity of this color is linearly proportional to the concentration. Objective detection and quantification of arginine were accomplished using image analysis software (freeware) based on this colorimetric result. The mean concentrations obtained in a blind study were 96.4 ± 5.1 µM for samples from female donors and 55.3 ± 5.3 µM for samples from males. These were not statistically different from the literature values of 94.8 µM ± 12.9 µM for females (p = 0.908) and 54.0 ± 12.6 µM for males (p = 0.914), respectively (± SEM in all cases). Conversely, the experimental means from males and female samples were statistically different from each other (p < 0.001). Objective differentiation between male and female fingermark deposits was achieved in a blind study with 93% accuracy. Additionally, the method was compatible both with samples lifted from common surfaces and with magnetically-powdered samples.
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spelling pubmed-80714982021-04-26 Microfluidic Device for the Identification of Biological Sex by Analysis of Latent Fingermark Deposits Marshall, Jamila S. Sita, Madelyn L. Landers, James P. Micromachines (Basel) Article To date, most research regarding amino acid detection and quantification in fingermarks relies on spectrometric methods. Herein, the Sakaguchi colorimetric test was adapted to a rotationally-driven microfluidic platform and used to detect and quantify arginine in fingermarks deposited by male and female donors. A red color indicates the presence of arginine in a given sample following the reaction, and the intensity of this color is linearly proportional to the concentration. Objective detection and quantification of arginine were accomplished using image analysis software (freeware) based on this colorimetric result. The mean concentrations obtained in a blind study were 96.4 ± 5.1 µM for samples from female donors and 55.3 ± 5.3 µM for samples from males. These were not statistically different from the literature values of 94.8 µM ± 12.9 µM for females (p = 0.908) and 54.0 ± 12.6 µM for males (p = 0.914), respectively (± SEM in all cases). Conversely, the experimental means from males and female samples were statistically different from each other (p < 0.001). Objective differentiation between male and female fingermark deposits was achieved in a blind study with 93% accuracy. Additionally, the method was compatible both with samples lifted from common surfaces and with magnetically-powdered samples. MDPI 2021-04-15 /pmc/articles/PMC8071498/ /pubmed/33921163 http://dx.doi.org/10.3390/mi12040442 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marshall, Jamila S.
Sita, Madelyn L.
Landers, James P.
Microfluidic Device for the Identification of Biological Sex by Analysis of Latent Fingermark Deposits
title Microfluidic Device for the Identification of Biological Sex by Analysis of Latent Fingermark Deposits
title_full Microfluidic Device for the Identification of Biological Sex by Analysis of Latent Fingermark Deposits
title_fullStr Microfluidic Device for the Identification of Biological Sex by Analysis of Latent Fingermark Deposits
title_full_unstemmed Microfluidic Device for the Identification of Biological Sex by Analysis of Latent Fingermark Deposits
title_short Microfluidic Device for the Identification of Biological Sex by Analysis of Latent Fingermark Deposits
title_sort microfluidic device for the identification of biological sex by analysis of latent fingermark deposits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071498/
https://www.ncbi.nlm.nih.gov/pubmed/33921163
http://dx.doi.org/10.3390/mi12040442
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