Cargando…

Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation

Polycaprolactone (PCL) is a well-known FDA approved biomaterial for tissue engineering. However, its hydrophobic properties limit its use for skin wound healing which makes its functionalization necessary. In this work, we present the fabrication and evaluation of PCL nanofibers by the electrospinni...

Descripción completa

Detalles Bibliográficos
Autores principales: Moyers-Montoya, Edgar D., Escobedo-González, René Gerardo, Vargas-Requena, Claudia L., Garcia-Casillas, Perla Elvia, Martínez-Pérez, Carlos A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071511/
https://www.ncbi.nlm.nih.gov/pubmed/33923388
http://dx.doi.org/10.3390/polym13081303
_version_ 1783683725261799424
author Moyers-Montoya, Edgar D.
Escobedo-González, René Gerardo
Vargas-Requena, Claudia L.
Garcia-Casillas, Perla Elvia
Martínez-Pérez, Carlos A.
author_facet Moyers-Montoya, Edgar D.
Escobedo-González, René Gerardo
Vargas-Requena, Claudia L.
Garcia-Casillas, Perla Elvia
Martínez-Pérez, Carlos A.
author_sort Moyers-Montoya, Edgar D.
collection PubMed
description Polycaprolactone (PCL) is a well-known FDA approved biomaterial for tissue engineering. However, its hydrophobic properties limit its use for skin wound healing which makes its functionalization necessary. In this work, we present the fabrication and evaluation of PCL nanofibers by the electrospinning technique, as well as PCL functionalized with 6-deoxy-6-amino-β-cyclodextrin (aminated nanofibers). Afterwards, epithelial growth factor (EGF) was anchored onto hydrophilic PCL/deoxy-6-amino-β-cyclodextrin. The characterization of the three electrospun fibers was made by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR); Confocal-Raman Spectroscopy were used for elucidated the chemical structure, the hydrophilicity was determined by Contact Angle (CA). In vitro cell proliferation test was made by seeding embryonic fibroblast cell line (3T3) onto the electrospun mats and in vivo studies in a murine model were conducted to prove its effectivity as skin wound healing material. The in vitro studies showed that aminated nanofibers without and with EGF had 100 and 150% more cell proliferation of 3T3 cells against the PCL alone, respectively. In vivo results showed that skin wound healing in a murine model was accelerated by the incorporation of the EGF. In addition, the EGF had favorable effects in epidermal cell proliferation. The study demonstrates that a protein of high biological interest like EGF can be attached covalently to the surface of a synthetic material enriched with amino groups. This kind of biomaterial has a great potential for applications in skin regeneration and wound healing.
format Online
Article
Text
id pubmed-8071511
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80715112021-04-26 Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation Moyers-Montoya, Edgar D. Escobedo-González, René Gerardo Vargas-Requena, Claudia L. Garcia-Casillas, Perla Elvia Martínez-Pérez, Carlos A. Polymers (Basel) Article Polycaprolactone (PCL) is a well-known FDA approved biomaterial for tissue engineering. However, its hydrophobic properties limit its use for skin wound healing which makes its functionalization necessary. In this work, we present the fabrication and evaluation of PCL nanofibers by the electrospinning technique, as well as PCL functionalized with 6-deoxy-6-amino-β-cyclodextrin (aminated nanofibers). Afterwards, epithelial growth factor (EGF) was anchored onto hydrophilic PCL/deoxy-6-amino-β-cyclodextrin. The characterization of the three electrospun fibers was made by means of field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR); Confocal-Raman Spectroscopy were used for elucidated the chemical structure, the hydrophilicity was determined by Contact Angle (CA). In vitro cell proliferation test was made by seeding embryonic fibroblast cell line (3T3) onto the electrospun mats and in vivo studies in a murine model were conducted to prove its effectivity as skin wound healing material. The in vitro studies showed that aminated nanofibers without and with EGF had 100 and 150% more cell proliferation of 3T3 cells against the PCL alone, respectively. In vivo results showed that skin wound healing in a murine model was accelerated by the incorporation of the EGF. In addition, the EGF had favorable effects in epidermal cell proliferation. The study demonstrates that a protein of high biological interest like EGF can be attached covalently to the surface of a synthetic material enriched with amino groups. This kind of biomaterial has a great potential for applications in skin regeneration and wound healing. MDPI 2021-04-16 /pmc/articles/PMC8071511/ /pubmed/33923388 http://dx.doi.org/10.3390/polym13081303 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moyers-Montoya, Edgar D.
Escobedo-González, René Gerardo
Vargas-Requena, Claudia L.
Garcia-Casillas, Perla Elvia
Martínez-Pérez, Carlos A.
Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation
title Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation
title_full Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation
title_fullStr Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation
title_full_unstemmed Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation
title_short Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino-β-cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation
title_sort epithelial growth factor-anchored on polycaprolactone/6-deoxy-6-amino-β-cyclodextrin nanofibers: in vitro and in vivo evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071511/
https://www.ncbi.nlm.nih.gov/pubmed/33923388
http://dx.doi.org/10.3390/polym13081303
work_keys_str_mv AT moyersmontoyaedgard epithelialgrowthfactoranchoredonpolycaprolactone6deoxy6aminobcyclodextrinnanofibersinvitroandinvivoevaluation
AT escobedogonzalezrenegerardo epithelialgrowthfactoranchoredonpolycaprolactone6deoxy6aminobcyclodextrinnanofibersinvitroandinvivoevaluation
AT vargasrequenaclaudial epithelialgrowthfactoranchoredonpolycaprolactone6deoxy6aminobcyclodextrinnanofibersinvitroandinvivoevaluation
AT garciacasillasperlaelvia epithelialgrowthfactoranchoredonpolycaprolactone6deoxy6aminobcyclodextrinnanofibersinvitroandinvivoevaluation
AT martinezperezcarlosa epithelialgrowthfactoranchoredonpolycaprolactone6deoxy6aminobcyclodextrinnanofibersinvitroandinvivoevaluation