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Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers

Given the fatal health conditions caused by emerging infectious pathogens, such as severe acute respiratory syndrome coronavirus 2, their rapid diagnosis is required for preventing secondary infections and guiding correct treatments. Although various molecular diagnostic methods based on nucleic aci...

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Autores principales: Park, Byungjun, Koo, Bonhan, Kim, Jisub, Lee, Kiri, Bang, Hyeonjin, Kim, Sung-Han, Jhang, Kyung Young, Shin, Yong, Lee, Seungrag
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071528/
https://www.ncbi.nlm.nih.gov/pubmed/33921114
http://dx.doi.org/10.3390/bios11040125
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author Park, Byungjun
Koo, Bonhan
Kim, Jisub
Lee, Kiri
Bang, Hyeonjin
Kim, Sung-Han
Jhang, Kyung Young
Shin, Yong
Lee, Seungrag
author_facet Park, Byungjun
Koo, Bonhan
Kim, Jisub
Lee, Kiri
Bang, Hyeonjin
Kim, Sung-Han
Jhang, Kyung Young
Shin, Yong
Lee, Seungrag
author_sort Park, Byungjun
collection PubMed
description Given the fatal health conditions caused by emerging infectious pathogens, such as severe acute respiratory syndrome coronavirus 2, their rapid diagnosis is required for preventing secondary infections and guiding correct treatments. Although various molecular diagnostic methods based on nucleic acid amplification have been suggested as gold standards for identifying different species, these methods are not suitable for the rapid diagnosis of pathogens owing to their long result acquisition times and complexity. In this study, we developed a rapid bio-optical sensor that uses a ball-lensed optical fiber (BLOF) probe and an automatic analysis platform to precisely diagnose infectious pathogens. The BLOF probe is easy to align and has a high optical sensing sensitivity (1.5-fold) and a large detection range (1.2-fold) for an automatic optical sensing system. Automatic signal processing of up to 250 copies/reaction of DNA of Q-fever-causing Coxiella burnetii was achieved within 8 min. The clinical utility of this system was demonstrated with 18 clinical specimens (9 Q-fever and 9 other febrile disease samples) by measuring the resonant wavelength shift of positive or negative samples for Coxiella burnetii DNA. The results from the system revealed the stable and automatic optical signal measurement of DNA with 100% accuracy. We envision that this BLOF probe-based sensor would be a practical tool for the rapid, simple, and sensitive diagnosis of emerging infectious pathogens.
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spelling pubmed-80715282021-04-26 Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers Park, Byungjun Koo, Bonhan Kim, Jisub Lee, Kiri Bang, Hyeonjin Kim, Sung-Han Jhang, Kyung Young Shin, Yong Lee, Seungrag Biosensors (Basel) Article Given the fatal health conditions caused by emerging infectious pathogens, such as severe acute respiratory syndrome coronavirus 2, their rapid diagnosis is required for preventing secondary infections and guiding correct treatments. Although various molecular diagnostic methods based on nucleic acid amplification have been suggested as gold standards for identifying different species, these methods are not suitable for the rapid diagnosis of pathogens owing to their long result acquisition times and complexity. In this study, we developed a rapid bio-optical sensor that uses a ball-lensed optical fiber (BLOF) probe and an automatic analysis platform to precisely diagnose infectious pathogens. The BLOF probe is easy to align and has a high optical sensing sensitivity (1.5-fold) and a large detection range (1.2-fold) for an automatic optical sensing system. Automatic signal processing of up to 250 copies/reaction of DNA of Q-fever-causing Coxiella burnetii was achieved within 8 min. The clinical utility of this system was demonstrated with 18 clinical specimens (9 Q-fever and 9 other febrile disease samples) by measuring the resonant wavelength shift of positive or negative samples for Coxiella burnetii DNA. The results from the system revealed the stable and automatic optical signal measurement of DNA with 100% accuracy. We envision that this BLOF probe-based sensor would be a practical tool for the rapid, simple, and sensitive diagnosis of emerging infectious pathogens. MDPI 2021-04-15 /pmc/articles/PMC8071528/ /pubmed/33921114 http://dx.doi.org/10.3390/bios11040125 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Byungjun
Koo, Bonhan
Kim, Jisub
Lee, Kiri
Bang, Hyeonjin
Kim, Sung-Han
Jhang, Kyung Young
Shin, Yong
Lee, Seungrag
Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers
title Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers
title_full Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers
title_fullStr Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers
title_full_unstemmed Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers
title_short Rapid Molecular Diagnostic Sensor Based on Ball-Lensed Optical Fibers
title_sort rapid molecular diagnostic sensor based on ball-lensed optical fibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071528/
https://www.ncbi.nlm.nih.gov/pubmed/33921114
http://dx.doi.org/10.3390/bios11040125
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