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Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle

Communication among epididymal epithelial cells creates the best luminal condition where spermatozoa mature, transport and are stored. Vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) have been used as signal indicators for clear and basal cells of the epididymal epithelium, respectively, in mice...

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Autores principales: Kim, Sung Woo, Kim, Bongki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Animal Sciences and Technology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071741/
https://www.ncbi.nlm.nih.gov/pubmed/33987602
http://dx.doi.org/10.5187/jast.2021.e32
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author Kim, Sung Woo
Kim, Bongki
author_facet Kim, Sung Woo
Kim, Bongki
author_sort Kim, Sung Woo
collection PubMed
description Communication among epididymal epithelial cells creates the best luminal condition where spermatozoa mature, transport and are stored. Vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) have been used as signal indicators for clear and basal cells of the epididymal epithelium, respectively, in mice, rats, bats, and pigs; however, these two markers have not yet been described in the epididymis of bulls. Here, we examined the presence and distribution of the B1 subunit of V-ATPase (B1-VATPase) and KRT5 in the distinct regions of adult bovine epididymides, specifically, the caput, corpus, and cauda. Immunofluorescence staining and confocal microscopy showed that narrow shaped-clear cells were placed in the caput and corpus regions of the bovine epididymis; however, they were absent in the cauda epididymis. In addition, B1-VATPase was highly expressed in the cauda spermatozoa; however, it was rarely detected in the caput spermatozoa. On the other hand, KRT5-positive cells, basal cells, were maintained beneath the basal lamina and they had the traditional form with a dome-shaped morphology from the caput to cauda region of the bovine epididymis. The co-expression of B1-VATPase and KRT5 was confined to basal cells placed in the basal region of the epithelium. In summary, 1) clear cells were present with region-specific localization, 2) B1-VATPase was present in the corpus and cauda spermatozoa but absent in the caput, 3) co-expressed cells with B1-VATPase and KRT5 were present in the adult bovine epididymis, and 4) B1-VATPase was not a specific marker for clear cells in the bovine epididymis. Therefore, the perfect epididymal luminal condition created by the specific expression and localization patterns of B1-VATPase might be necessary to obtain fertilizing capacity of spermatozoa in the bovine epididymis.
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spelling pubmed-80717412021-05-05 Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle Kim, Sung Woo Kim, Bongki J Anim Sci Technol Research Article Communication among epididymal epithelial cells creates the best luminal condition where spermatozoa mature, transport and are stored. Vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) have been used as signal indicators for clear and basal cells of the epididymal epithelium, respectively, in mice, rats, bats, and pigs; however, these two markers have not yet been described in the epididymis of bulls. Here, we examined the presence and distribution of the B1 subunit of V-ATPase (B1-VATPase) and KRT5 in the distinct regions of adult bovine epididymides, specifically, the caput, corpus, and cauda. Immunofluorescence staining and confocal microscopy showed that narrow shaped-clear cells were placed in the caput and corpus regions of the bovine epididymis; however, they were absent in the cauda epididymis. In addition, B1-VATPase was highly expressed in the cauda spermatozoa; however, it was rarely detected in the caput spermatozoa. On the other hand, KRT5-positive cells, basal cells, were maintained beneath the basal lamina and they had the traditional form with a dome-shaped morphology from the caput to cauda region of the bovine epididymis. The co-expression of B1-VATPase and KRT5 was confined to basal cells placed in the basal region of the epithelium. In summary, 1) clear cells were present with region-specific localization, 2) B1-VATPase was present in the corpus and cauda spermatozoa but absent in the caput, 3) co-expressed cells with B1-VATPase and KRT5 were present in the adult bovine epididymis, and 4) B1-VATPase was not a specific marker for clear cells in the bovine epididymis. Therefore, the perfect epididymal luminal condition created by the specific expression and localization patterns of B1-VATPase might be necessary to obtain fertilizing capacity of spermatozoa in the bovine epididymis. Korean Society of Animal Sciences and Technology 2021-03 2021-03-31 /pmc/articles/PMC8071741/ /pubmed/33987602 http://dx.doi.org/10.5187/jast.2021.e32 Text en © Copyright 2021 Korean Society of Animal Science and Technology https://creativecommons.org/licenses/by-nc/4.0/This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Sung Woo
Kim, Bongki
Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle
title Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle
title_full Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle
title_fullStr Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle
title_full_unstemmed Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle
title_short Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle
title_sort vacuolar h(+)-atpase is not restricted to clear cells of the epididymal epithelium in cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071741/
https://www.ncbi.nlm.nih.gov/pubmed/33987602
http://dx.doi.org/10.5187/jast.2021.e32
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