Membrane-embedded TSPO: an NMR view

Translocator Protein (18 kDa) (TSPO) is a mitochondrial transmembrane protein commonly used as a biomarker for neuroinflammation and is also a potential therapeutic target in neurodegenerative diseases. Despite intensive research efforts, the function of TSPO is still largely enigmatic. Deciphering...

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Autores principales: Rivière, Gwladys, Jaipuria, Garima, Andreas, Loren B., Leonov, Andrei, Giller, Karin, Becker, Stefan, Zweckstetter, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071791/
https://www.ncbi.nlm.nih.gov/pubmed/33354729
http://dx.doi.org/10.1007/s00249-020-01487-0
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author Rivière, Gwladys
Jaipuria, Garima
Andreas, Loren B.
Leonov, Andrei
Giller, Karin
Becker, Stefan
Zweckstetter, Markus
author_facet Rivière, Gwladys
Jaipuria, Garima
Andreas, Loren B.
Leonov, Andrei
Giller, Karin
Becker, Stefan
Zweckstetter, Markus
author_sort Rivière, Gwladys
collection PubMed
description Translocator Protein (18 kDa) (TSPO) is a mitochondrial transmembrane protein commonly used as a biomarker for neuroinflammation and is also a potential therapeutic target in neurodegenerative diseases. Despite intensive research efforts, the function of TSPO is still largely enigmatic. Deciphering TSPO structure in the native lipid environment is essential to gain insight into its cellular activities and to design improved diagnostic and therapeutic ligands. Here, we discuss the influence of lipid composition on the structure of mammalian TSPO embedded into lipid bilayers on the basis of solid-state NMR experiments. We further highlight that cholesterol can influence both the tertiary and quaternary TSPO structure and also influence TSPO localization in mitochondria-associated endoplasmic reticulum membranes.
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spelling pubmed-80717912021-05-05 Membrane-embedded TSPO: an NMR view Rivière, Gwladys Jaipuria, Garima Andreas, Loren B. Leonov, Andrei Giller, Karin Becker, Stefan Zweckstetter, Markus Eur Biophys J Original Article Translocator Protein (18 kDa) (TSPO) is a mitochondrial transmembrane protein commonly used as a biomarker for neuroinflammation and is also a potential therapeutic target in neurodegenerative diseases. Despite intensive research efforts, the function of TSPO is still largely enigmatic. Deciphering TSPO structure in the native lipid environment is essential to gain insight into its cellular activities and to design improved diagnostic and therapeutic ligands. Here, we discuss the influence of lipid composition on the structure of mammalian TSPO embedded into lipid bilayers on the basis of solid-state NMR experiments. We further highlight that cholesterol can influence both the tertiary and quaternary TSPO structure and also influence TSPO localization in mitochondria-associated endoplasmic reticulum membranes. Springer International Publishing 2020-12-22 2021 /pmc/articles/PMC8071791/ /pubmed/33354729 http://dx.doi.org/10.1007/s00249-020-01487-0 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Rivière, Gwladys
Jaipuria, Garima
Andreas, Loren B.
Leonov, Andrei
Giller, Karin
Becker, Stefan
Zweckstetter, Markus
Membrane-embedded TSPO: an NMR view
title Membrane-embedded TSPO: an NMR view
title_full Membrane-embedded TSPO: an NMR view
title_fullStr Membrane-embedded TSPO: an NMR view
title_full_unstemmed Membrane-embedded TSPO: an NMR view
title_short Membrane-embedded TSPO: an NMR view
title_sort membrane-embedded tspo: an nmr view
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071791/
https://www.ncbi.nlm.nih.gov/pubmed/33354729
http://dx.doi.org/10.1007/s00249-020-01487-0
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