Analysis of patchclamp recordings: model-free multiscale methods and software

Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-pa...

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Detalles Bibliográficos
Autores principales: Pein, Florian, Eltzner, Benjamin, Munk, Axel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071803/
https://www.ncbi.nlm.nih.gov/pubmed/33837454
http://dx.doi.org/10.1007/s00249-021-01506-8
Descripción
Sumario:Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-package and as a graphical user interface. This includes selection of the right approach and tuning of parameters. We also discuss advantages and disadvantages of model-free approaches in comparison to hidden Markov model approaches and explain how they complement each other. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00249-021-01506-8.

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